Abstract: In one form, a two-directional scanning method for luminescence microscopy is disclosed. A series of continuous scans are performed by an interrogation wide-field relative to a first direction and a target is identified. A precise position of the target is determined in the first direction. At least one scan by the interrogation wide-field is performed relative to a second direction at or near the precise position of the target in the first direction. The two-directional scanning method produces “on-the-fly” (i.e. ex tempore or impromptu) precise localization of targets. Embodiments open up new applications for background-free or background-reduced luminescence microscopy, for example time-gated or time-resolved luminescence microscopy, in a relatively fast, higher speed or more efficient manner.

Abstract: A continuous wave Raman laser for producing visible laser output comprising: a resonator cavity; at least a first reflector and a second reflector said first and second reflectors being located at opposite ends of the resonator cavity; a laser gain medium located in the resonator cavity for generating a continuous wave fundamental beam which resonates within the resonator cavity when pumped by a pump beam from a pump source external to the resonator cavity; a solid Raman-active medium positioned in the resonator cavity for Raman shifting the fundamental beam to produce a continuous wave Raman beam which resonates within the resonator cavity; and a non-linear medium positioned in the resonator cavity for frequency converting the continuous wave Raman beam to a converted wavelength to produce a continuous wave converted beam.

Abstract: A fluorescence detection system comprises a light source (22), dichroic mirror (32), excitation port (16), emission port (14), and a detector. The light source (22) is, for example, a pulsed ultraviolet LED, with a light emission that decays sufficiently rapidly to permit gated detection of fluorescence from a fluorescently-labelled species, at a time when it is distinguishable from autofluorescence. The detector is, for example, an electron multiplying CCD, with high gain on-chip amplification. A circuit (26) may be used to control a repeating cycle of (i) generation of a 20-200 microsecond UV. pulse; (ii) a gate delay of 1-5 microseconds; and (iii) a 10-800 microsecond detection period. This allows time-resolved-fluorescence-microscopy with real time or near real time operation.

Abstract: The invention provides a laser system (100) wherein the output may be selected from two or more different wavelengths of output laser light. The system (100) comprises a laser capable of having at least two different wavelengths of laser light resonating in the cavity (105) simultaneously. One of the frequencies is generated by a Raman crystal (135) which shifts the frequency of light generated by the lasing medium (125). A tunable non-linear medium (140), such as LBO, is provided in the cavity for selectively frequency converting at least one of the at least two different wavelengths of laser light. The conversion may be SHG, SFG or DFG for example. A tuner (145) is provided to tune the non-linear medium to select the particular wavelength to convert. Temperature tuning or angle tuning of the non-linear medium can be used. A Q switch (130) may also be provided in the cavity.

Abstract: A fluorescence detection system comprises a light source (22), dichroic mirror (32), excitation port (16), emission port (14), and a detector. The light source (22) is, for example, a pulsed ultraviolet LED, with a light emission that decays sufficiently rapidly to permit gated detection of fluorescence from a fluorescently-labelled species, at a time when it is distinguishable from autofluorescence. The detector is, for example, an electron multiplying CCD, with high gain on-chip amplification. A circuit (26) may be used to control a repeating cycle of (i) generation of a 20-200 microsecond UV. pulse; (ii) a gate delay of 1-5 microseconds; and (iii) a 10-800 microsecond detection period. This allows time-resolved-fluorescence-microscopy with real time or near real time operation.

Abstract: The invention provides a laser system (100) wherein the output may be selected from two or more different wavelengths of output laser light. The system (100) comprises a laser capable of having at least two different wavelengths of laser light resonating in the cavity (105) simultaneously. One of the frequencies is generated by a Raman crystal (135) which shifts the frequency of light generated by the lasing medium (125). A tunable non-linear medium (140), such as LBO, is provided in the cavity for selectively frequency converting at least one of the at least two different wavelengths of laser light. The conversion may be SHG, SFG or DFG for example. A tuner (145) is provided to tune the non-linear medium to select the particular wavelength to convert. Temperature tuning or angle tuning of the non-linear medium can be used. A Q switch (130) may also be provided in the cavity.

Abstract: A method for joining tissue comprising aligning and abutting edges of the tissue to be joined applying a biodegradable, biological solder or an analogue thereof, across the edges and exposing the solder to an energy source under conditions which provide transfer of energy from the source to the solder to cause the solder to bond to the tissue surface adjacent the edges to provide a weld holding the edges together.

Abstract: A method for joining tissue comprising aligning and abutting edges of the tissue to be joined applying a biodegradable, biological solder or an analogue thereof, across the edges and exposing the solder to an energy source under conditions which provide transfer of energy from the source to the solder to cause the solder to bond to the tissue surface adjacent the edges to provide a weld holding the edges together.

Abstract: A method for joining tissue comprising aligning and abutting edges of the tissue to be joined applying biodegradable, biological solder or an analogue thereof, across the edges and exposing the solder to an energy source under conditions which provide transfer of energy from the source to the solder to cause the solder to bond to the tissue surface adjacent the edges to provide a weld holding the edges together.

Abstract: There is disclosed a metal vapour laser comprising a discharge tube having a buffer gas therein and operating at high temperature, the buffer gas including a laser output power enhancing substance in an amount sufficient to substantially increase the power output of the laser. There is also disclosed a process for operating a metal vapour laser of the invention.