Abstract: High-throughput column arrays of vascularized living parenchyma/tissue having pillars dispersed in specialized configurations and arrangements substantially vertically through the column to provide support, passive or active perfusion, and access to internal portions of tissue for analytical sampling needs, along with 3-D printing methods of manufacture and analytical screening methods employing the column arrays.

Abstract: High-throughput column arrays of vascularized living parenchyma/tissue having pillars dispersed in specialized configurations and arrangements substantially vertically through the column to provide support, passive or active perfusion, and access to internal portions of tissue for analytical sampling needs, along with 3-D printing methods of manufacture and analytical screening methods employing the column arrays.

Abstract: The present invention generally relates to methods, compositions and uses thereof for enhancing vascularization of a tissue or cell transplant for transplantation into a subject. In particular, one aspect of the present invention provides methods and compositions comprising the use of a population of stromal vascular fraction (SVF) cells to encapsulate or surround a tissue or cell transplant to enhance vascularization of the tissue or cell transplant. Another aspect of the present invention provides methods and compositions for enhancing vascularization of a tissue or cell transplant by combining a population of SVF cells with a tissue or cell transplant to form a transplant mixed with SVF cells. In some embodiments, the SVF cells can be on the surface or embedded within a three-dimensional matrix. In some embodiments, the SVF cells can be generically engineered to secrete therapeutic proteins or pro-angiogenic factors.