Abstract: Insulin (INS) analogs are disclosed including modifications that increase half-life when compared to native, human INS, that maintain selectivity to the insulin receptor (IR) and that provide in vitro and in vivo stability for improved druggability properties and less immunogenicity. Pharmaceutical compositions also are disclosed that include one or more of the INS analogs described herein in a pharmaceutically acceptable carrier. Methods of making and using the INS analogs also are disclosed, especially for treating metabolic CA conditions, diseases or disorders, especially treating diseases such as diabetes and obesity.

Abstract: Method and apparatus are set forth for providing a substantially uniform coating on inner wall portions of a die having slots or openings formed in the face of the die. The die is supported by a fixture for up and down movement while it is immersed in a bath of a desired plating solution. A tube or cylinder positioned on an upper surface portion of the die repeatedly collects a column of plating material in a cylinder from the bath on downward movements of the die, so as to provide fresh or replenishment plating solution to the die. The column of plating solution in the cylinder functions as a hydrostatic head and forces the confined solution into the interior of the die on upward movements, thus promoting coating uniformity along inner walls of the die. The die may be rotated 180 degrees so that each face of the die may be subjected to the hydrostatic force flow of the plating solution, also resulting in a more uniform coating.