Abstract: Methods are included for quantitating the efficacy of oral care products at dislodging cells from biofilm test surfaces or inhibit or delay the accumulation of cells on a test surface. More specifically, the inventive methods measure the effectiveness of compositions and appliances in a test environment that models biofilm surface orientations that are encountered on a tooth surface, i.e. interproximal and subgingival tooth surfaces. The use of test surfaces that are removably attachable to a tooth prosthesis allow a variety of different quantitative methods to be applied to determine the amount of cells removed or deposited in a biofilm.

Abstract: A brushhead for an acoustic powered toothbrush having a particular bristle configuration which includes a plurality of bristle portions arranged in four columns and eight rows, wherein the distal end two adjacent rows and the proximal end two adjacent rows both have longer bristles than the remaining bristle portions except for an intermediate row of bristle portions which are slightly longer than the longer bristle portions. The distal, intermediate and proximal bristle portions all have pointed bristle portion tips.