Abstract: The present invention is directed to methods and compositions for evaluating allelic variations with improved quality controls, methods of preparing such compositions, and applications employing such compositions and methods. In particular, the present invention provides methods and compositions for reducing false positives and/or false negatives in nucleic acid measurements.

Abstract: The present invention relates to methods and compositions for identifying biomarkers that indicate a biological state, in particular cancer or predisposition to cancer.

Abstract: The present invention relates to methods, compositions, and kits for identifying biomarkers useful in characterizing biological states. In particular, the invention relates to methods and compositions for molecular characterization of biological states by gene expression profiling. The invention also relates to assessing effects of DNA polymorphisms on regulation of transcription. The biomarkers and polymorphisms identified find use in diagnostic and treatment approaches, e.g., some embodiments of the invention provide methods and kits for detecting bronchogenic carcinoma and risks thereof.

Abstract: The present invention is directed to methods and compositions for evaluating nucleic acids, methods of preparing such compositions, and applications and business methods employing such compositions and methods. In particular, the present invention provides business methods for operating a gene expression measurement service.

Abstract: The present disclosure relates to methods and compositions for identifying biomarkers that indicate a biological state, in particular cancer or predisposition to cancer. Also provided are methods and compositions for identifying a greater risk for a cancer-related condition in an subject.

Abstract: The present invention is directed to methods and compositions for evaluating nucleic acids, methods of preparing such compositions, and applications and business methods employing such compositions and methods. In particular, the present invention provides business methods for operating a gene expression measurement service.

Abstract: The present invention is directed to methods and compositions for assaying one or more analytes in a sample with improved sensitivity, reproducibility and/or lower detection limits, as well as applications employing such compositions and methods. In particular, the present invention provides methods and compositions for assaying one or more non-nucleic acid analytes with one or more other nucleic acid and/or non-nucleic acid analytes that serve as controls.

Abstract: A method measure expression of multiple target genes in a progenitor cell for bronchogenic carcinoma comprising the use of reverse transcription-polymerase chain reaction (RT-PCR) to allow simultaneous expression measurement of the multiple target genes is disclosed.

Abstract: A method measure expression of multiple target genes in a progenitor cell for bronchogenic carcinoma comprising the use of reverse transcription-polymerase chain reaction (RT-PCR) to allow simultaneous expression measurement of the multiple target genes is disclosed.

Abstract: A method for direct comparison of numerical gene expression values between samples of genes using reverse transcription-polymerase chain reaction is described. cDNA, a competitive template mixture, and primer pairs for a plurality of genes are combined with at least one suitable buffer and at least one suitable enzyme to form a mixture. The mixture is amplified for a predetermined number of cycles to form PCR products. The PCR products are mixed with at least one suitable buffer, at least one enzyme, and one primer pair specific for each of the genes. The resulting mixture is amplified an additional predetermined number of cycles.

Abstract: A method and apparatus for quantitative measurement of gene expression through multiplex competitive reverse transcriptase polymerase chain reaction amplification are disclosed. The method and apparatus are especially useful for analysis of small specimens of cells and tissues.

Abstract: A method for quantitative measurement of gene expression through multiplex competitive reverse transcriptase polymerase chain reaction amplification has been established which comprises isolating cellular mRNA of a target gene and a housekeeping gene which are then reverse transcribed and specifically amplified in the presence of competitive templates such that a ratio of target to housekeeping gene is obtained and used to assess the amount of gene expression. This method is useful for analysis of the small specimens (cells and tissues) available from human subjects and will allow improved understanding of mechanisms of disease.

Abstract: A method for quantitative measurement of gene expression through multiplex competitive reverse transcriptase polymerase chain reaction amplification is established. This method is especially useful for analysis of small specimens of cells and tissues.

Abstract: Human bronchial epithelial cell lines permanently transformed by human papilloma viruses have been obtained. These cell lines are useful for the study of growth and differentiation in bronchial carcinoma and the identification of chemical and biological agents that may be useful in the therapy of human lung cancer.