Abstract: Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.

Abstract: The present invention is directed to recombinant thraustochytrids that grow on sucrose and cell cultures comprising the recombinant thraustochytrids as well as methods of producing cell cultures, biomasses, microbial oils, compositions, and biofuels using the recombinant thraustochytrids.

Abstract: The present invention is directed to recombinant microalgal cells and their use in production of heterologous hemagglutinin-neuraminidase (HN) polypeptides, as well as compositions and uses thereof.

Abstract: The present invention is directed to recombinant thraustochytrids that grow on xylose and cell cultures comprising the recombinant thraustochytrids as well as methods of producing cell cultures, biomasses, microbial oils, compositions, and biofuels using the recombinant thraustochytrids.

Abstract: The present invention relates to recombinant microalgal cells and their use in heterologous protein production, methods of production of heterologous polypeptides in microalgal extracellular bodies, microalgal extracellular bodies comprising heterologous polypeptides, and compositions comprising the same.

Abstract: Most microalgae are obligate photoautotrophs and their growth is strictly dependent on the generation of photosynthetically-derived energy. In this study it is shown that the microalga Phaeodaclylurn tricornutum can be engineered to import glucose and grow in the dark through the introduction of genes encoding glucose transporters. Both the human and Chlorella kessleri glucose transporters facilitated the uptake of glucose by P. tricornutum, allowing the cells to metabolize exogenous organic carbon and thrive, independent of light. This is the first successful trophic conversion of an obligate photoautotroph through metabolic engineering, and it demonstrates that methods of cell nourishment can be fundamentally altered with the introduction of a single gene. Since strains transformed with the glucose transport genes are able to grow non-photosynthetically, they can be exploited for the analysis of photosynthetic processes through mutant generation and characterization.

Abstract: A human-machine interface assembly includes a user interface and a passive force feedback mechanism. The user interface is configured, upon receipt of an input force that exceeds a null breakout force, to move from a null position to a first control position and, upon receipt of an input force that exceeds a soft stop force, to move beyond the first control position. The passive force feedback mechanism is coupled to the user interface and is configured to supply the null breakout force to the user interface when the user interface is in the null position, and supply the soft stop force to the user interface when the user interface is in the first control position. The soft stop force exceeds the null breakout force and is not supplied to the user interface unless the user interface is in the first control position.

Abstract: Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.

Abstract: Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.

Abstract: Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.

Abstract: The present invention relates to recombinant cells and microorganisms of the phylum Labyrinthulomycota and their use in heterologous protein production. Novel promoter, terminator, and signal sequences for efficient production and, optionally, secretion of polypeptides from recombinant host cells and microorganisms are also encompassed by the present invention.

Abstract: Disclosed are novel acyl-CoA synthetases and novel acyltransferases, nucleic acid molecules encoding the same, recombinant nucleic acid molecules and recombinant host cells comprising such nucleic acid molecules, genetically modified organisms (microorganisms and plants) comprising the same, and methods of making and using the same. Also disclosed are genetically modified organisms (e.g., plants, microorganisms) that have been genetically modified to express a PKS-like system for the production of PUFAs (a PUFA PKS system or PUFA synthase), wherein the organisms have been modified to express an acyl-CoA synthetase, to express an acyl transferase, to delete or inactivate a fatty acid synthase (FAS) expressed by the organism, to reduce competition for malonyl CoA with the PUFA synthase or to increase the level of malonyl CoA in the organism, and in one aspect, to inhibit KASII or KASIII.

Abstract: Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.

Abstract: The present invention describes a method for production of a renewable, combustible liquid fuel that may be used in internal combustion engines, as a fuel source for electricity generation including turbines and fuel cells, or as a burnable heat source. This fuel is derived from set of biomolecules that are produced under nutrient limitation conditions as those found at a waste water treatment plant. More specifically this invention envisions the use of poly(3-hydroxyalkanoates) (PHA), especially those with monomeric residues ranging in size from C4 to C10 , as feed stream for production of a biofuel.

Abstract: The present invention relates to the use of a naturally occurring group of biological compounds to produce a combustible fuel that may be used in either internal combustion engines or as a burnable heat source. These compounds are a set of biomolecules produced by some bacteria in nutrient limiting environmental conditions when storage of carbon is necessary. More specifically this invention envisions the use of polyhydroxy alkanoates (PHA), especially those ranging in size from C4 to C8, to produce the combustible fuel.

Abstract: A human-machine interface assembly includes a user interface, a first null breakout force mechanism, and a second null breakout force mechanism. The first null breakout force mechanism is coupled to the user interface and is configured to supply the null breakout force to the user interface at a first force magnitude when the user interface is in the null position. The second null break out force mechanism is adapted to receive an engagement signal and is operable, in response to the engagement signal, to engage the user interface and supply the null breakout force to the user interface at a second force magnitude, which is greater than the first force magnitude, when the user interface is in the null position.

Abstract: A human-machine interface assembly includes a user interface and a passive force feedback mechanism. The user interface is configured, upon receipt of an input force that exceeds a null breakout force, to move from a null position to a first control position and, upon receipt of an input force that exceeds a soft stop force, to move beyond the first control position. The passive force feedback mechanism is coupled to the user interface and is configured to supply the null breakout force to the user interface when the user interface is in the null position, and supply the soft stop force to the user interface when the user interface is in the first control position. The soft stop force exceeds the null breakout force and is not supplied to the user interface unless the user interface is in the first control position.

Abstract: A user interface fluid linkage system includes first and second user interfaces that are linked by a single, constant volume fluid line. The system includes identically configured, but oppositely mounted hydraulic fluid chambers to link motion between the first and second user interfaces.

Abstract: The present invention provides biological oils and methods and uses thereof. The biological oils are preferably produced by heterotrophic fermentation of one or more microorganisms using cellulose-containing feedstock as a main source of carbon. The present invention also provides methods of producing lipid-based biofuels and food, nutritional, and pharmaceutical products using the biological oils.

Abstract: A serviceable gas/liquid separator is provided. The gas/liquid separator has a cross-section with one long axis and one short axis, with an aspect ratio of at least 1.3. A preferred cross-section, is an elliptical cross-section. Assemblies in which the separator unit can be used, are provided. In addition methods of use and construction are provided.