Abstract: A stoma covering device according to various aspects of the present technology may comprise an open ended body having an internal receiving area configured to receive and be positioned over the stoma to temporarily cover the stoma during the changing process of an ostomy device or wafer. The open ended body may be configured to be held in place in a hands-free manner and without the aid of adhesives, straps, or other mechanical devices. A resilient body may be attached to the open ended body and be used to create a low pressure region within the internal receiving area prior to the stoma covering device being positioned over the stoma.

Abstract: A stoma covering device according to various aspects of the present technology may comprise an open ended body having an internal receiving area configured to receive and be positioned over the stoma to temporarily cover the stoma during the changing process of an ostomy device or wafer. The open ended body may be configured to be held in place in a hands-free manner and without the aid of adhesives, straps, or other mechanical devices. A resilient body may be attached to the open ended body and be used to create a low pressure region within the internal receiving area prior to the stoma covering device being positioned over the stoma.

Abstract: Disclosed herein are genetically modified microorganisms and related methods for the enhanced export of oligosaccharides. The microorganisms described herein express major facility superfamily proteins such as CDT-1 which allows for the export of oligosaccharides. Variants of CDT-1 exhibit higher activity regarding oligosaccharide export. Means to export oligosaccharides into the growth medium are provided herein.

Abstract: Disclosed herein are genetically modified microorganisms and related methods for enhanced utilization of oligosaccharides and improved productivity of compounds derived from the metabolism of the oligosaccharides. The microorganisms described herein have altered activities of plasma membrane ATPase protein (PMA1) and/or one or more extracellular glucose sensors, namely, sucrose non-fermenting protein (SNF3), restores glucose transport protein (RGT2), and G protein-coupled receptor 1 protein (GPR1). These genetic modifications provide the microorganisms an increased ability to utilize an oligosaccharide to produce a compound of interest, particularly, tagatose, 2?-fucosyllactose, and psicose. Methods of culturing the microorganisms in the presence of such oligosaccharides to produce the products of interest are also provided.

Abstract: Embodiments disclosed herein include devices, methods, and systems for direct, selective, and sensitive detection of single-stranded target RNA sequences from various sources using a programmed Cas13a protein. When activated by binding a target RNA sequence, the Cas13a cleaves a tether releasing a reporter molecule that may then be detected. In some embodiments, the systems, methods, and devices may include a filter or membrane that may help to separate the tethered and untethered reporter molecules. These devices, systems, and techniques allow a user to rapidly process samples that may contain the target RNA, without needing to amplify the target sequences. These devices and methods may be used to assay a wide variety of samples and target RNA sources, for the presence or absence of a specific target RNA sequence. Compositions and kits, useful in practicing these methods, for example detecting a target RNA in a biological sample, are also described.