Abstract: The present invention relates to an expression system for the heterologous expression of a nucleic acid sequence of interest in a mammalian cell, the system comprising: (i) a first genetic entity, comprising: a nucleic acid sequence encoding a functional Epstein Barr virus nuclear antigen 1 (EBNA-1), the nucleic acid sequence being operably linked to regulatory elements that allow for expression of the nucleic acid sequence encoding a functional EBNA-1; (ii) a second genetic entity, comprising: a nucleic acid sequence encoding a functional nucleoside diphosphate kinase A (NDPK-A), the nucleic acid sequence being operably linked to regulatory elements that allow for expression of the nucleic acid sequence encoding a functional NDPK-A; (iii) a third genetic entity, comprising: the nucleic acid sequence of interest being operably linked to regulatory elements that allow for expression of the nucleic acid sequence of interest; and (iv) a four genetic entity, comprising: the Epstein Barr virus OriP sequence or one

Abstract: The present invention relates to an expression system for the heterologous expression of a nucleic acid sequence of interest in a mammalian cell, the system comprising: (i) a first genetic entity, comprising: a nucleic acid sequence encoding a functional Epstein Barr virus nuclear antigen 1 (EBNA-1), the nucleic acid sequence being operably linked to regulatory elements that allow for expression of the nucleic acid sequence encoding a functional EBNA-1; (ii) a second genetic entity, comprising: a nucleic acid sequence encoding a functional nucleoside diphosphate kinase A (NDPK-A), the nucleic acid sequence being operably linked to regulatory elements that allow for expression of the nucleic acid sequence encoding a functional NDPK-A; (iii) a third genetic entity, comprising: the nucleic acid sequence of interest being operably linked to regulatory elements that allow for expression of the nucleic acid sequence of interest; and (iv) a four genetic entity, comprising: the Epstein Barr virus OriP sequence or one

Abstract: The present invention is an electively detachably coupled ball joint and pivot cup combination for electively detachably coupling a mast to a surfboard. The electively detachably coupled ball joint and pivot cup combination includes a pivot cup which is pivotally and rotationally coupled to the surfboard and an annular plate which is disposed on and mechanically coupled to the peripheral edge of the opening of the pivot cup. The electively detachably coupled ball joint and pivot cup combination also includes a spherical member which has been cut into a plurality of sections and which, when not held apart, may be either inserted into or removed from the pivot cup through the annular plate with each of the plurality of sections of the spherical member being loosely coupled to the mast and a sleeve into which the mast is inserted and which is slidably coupled to the mast.

Abstract: The invention is a wind-propelled skateboard which includes a body member having roller bearing wheels adapted for transporting a skateboarder over a concrete or asphalt surface, a mast, which is detachably coupled to the body member in such a manner that it is substantially free from pivotal restraint, a boom coupled to the mast by a hinge, and a sail coupled to both the mast and the boom.