Abstract: The present disclosure generally relates to methods of protecting the genomic integrity and/or biological activity of AAV viral particles in a sample containing both AAV particles and helper virus particles during heat inactivation. The methods include heating, to a temperature greater than or equal to 45?C, a sample containing helper virus particles, AAV particles, and a buffer. The buffer includes a concentration of 10 mM or greater kosmotropic salts and/or a concentration of 10 mM or greater of divalent or trivalent cations.

Abstract: There is provided a method of producing a mixed metal compound comprising at least Mg2+ and at least Fe+ having an aluminium content of less than 10000 ppm, having an average crystal size of less than 20 nm (200 A) comprising the steps of: (a) combining a Mg2+ salt and a Fe3+ salt with Na2CO3 and NaOH to produce a slurry, wherein the pH of the slurry is maintained at from 9 5 to 1 1, and wherein the Na2CO3 is provided at an excess of 0 to 4.0 moles than is required to complete the reaction (b) subjecting the slurry to mixing under conditions providing a power per unit volume of 0 03 to 1.6 kW/m3 (c) separating the mixed metal compound from the slurry, to obtain a crude product having a dry solid content of at least 10 wt % (d) drying the crude product either by (i) heating the crude product to a temperature of no greater than 150° C. and sufficient to provide a water evaporation rate of 0.

Abstract: There is provided a method of producing a mixed metal compound comprising at least Mg2+ and at least Fe+ having an aluminium content of less than 10000 ppm, having an average crystal size of less than 20 nm (200 A) comprising the steps of: (a) combining a Mg2+ salt and a Fe3+ salt with Na2CO3 and NaOH to produce a slurry, wherein the pH of the slurry is maintained at from 9 5 to 1 1, and wherein the Na2CO3 is provided at an excess of 0 to 4.0 moles than is required to complete the reaction (b) subjecting the slurry to mixing under conditions providing a power per unit volume of 0 03 to 1.6 kW/m3 (c) separating the mixed metal compound from the slurry, to obtain a crude product having a dry solid content of at least 10 wt % (d) drying the crude product either by (i) heating the crude product to a temperature of no greater than 150° C. and sufficient to provide a water evaporation rate of 0.

Abstract: A composition comprising (A) 1,1,2,2,3-pentafluoropropane(CF2HCF2CFH2) and (B) at least one hydrofluorocarbon selected from the group consisting of 1,1,1,3,3-pentafluoropropane (CF3CH2CF2H) and 1,1,1,2,2,3,3,4,4-nonafluorobutane (CF3CF2CF2CF2H) is described. The composition is useful as a refrigerant and may be used in chillers as a replacement for refrigerant R-11.

Abstract: A composition comprising (A) 1,1,2,2,3-pentafluoropropane (CF 2 HCF 2 CFH 2) and (B) at least one hydrofluorocarbon selected from the group consisting of 1,1,1,3,3-pentafluoropropane (CF 3 CH 2 CF 2 H) and 1,1,1,2,2,3,3,4,4-nonafluorobutane (CF 3 CF 2 CF 2 C F 2 H) is described. The composition is useful as a refrigerant and may be used in chillers as a replacement for refrigerant R-11.

Abstract: ?PPA composition is described which comprises carbon dioxide (CO2), at least one hydrofluorocarbon selected from the group consisting of 1,1,1,2tetrafluoroethane (R-134a), 1,1,2,2-tetrafluoroethane (R-134) and 1,1difluoroethane (R-152a), and at least one hydrofluorocarbon selected from the group consisting of 1,1,1,3,3,3-hexafluoropropane (R-236fa) and 1,1,1,2,3,3-hexafluoropropane (R-236ea). The composition may additionally comprise at least one hydrocarbon and can be used as a replacement for refrigerant R-12 in automobile air conditioning and domestic refrigeration systems.

Abstract: The invention relates to improvements in a diver's semi-closed circuit breathing apparatus and is intended to better control the partial pressure of oxygen during descent and ascent. Such apparatus includes a counterlung, sources of oxygen and inert gas, an oxygen regulator, a mass flow jet in the oxygen circuit, a regulator for the inert gas, and a feed-back controlled laminar flow element for feeding a mixture of the oxygen and inert gas to a counterlung of the breathing apparatus. The improvement comprises the provision of an oxygen accumulator in the oxygen circuit following the mass flow jet so that a portion of the delivered oyxgen is diverted thereto during descent to counteract oxygen partial pressure overshoot. At constant depth the apparatus operates normally, maintaining appropriate oxygen partial pressure, and, during ascent, oxygen is returned from the accumulator to the breathing apparatus to counteract any drop in oxygen partial pressure.

Abstract: A tandem quadrupole-based mass spectrometer including a highly efficient intermediate fragmentation stage. The disclosed fragmentation stage employs collision-induced dissociation (CID), in an electrodynamic focus device, which may be a quadrupole operated in a broad band filter mode. The disclosed CID process occurs at low energy (e.g., less than 1 keV, but preferably 2 eV to 100 eV), and is quite different from the high energy (e.g, greater than 1 keV, and typically 3 keV to 10 keV) CID process in prior tandem instruments such as mass-analyzed ion kinetic energy spectrometry (MIKES) systems. The efficiency of the present CID fragmentation is as high as 65%.