Abstract: A test strip for confirming a desired proportion of components in dialysate, including a first medium capable of indicating the concentration of bicarbonate ion, and a second medium capable of indicating the concentration of glucose. The test strip defines a first region impregnated with the first medium, and a second region impregnated with the second medium. The test strip optionally includes a third medium capable of indicating the pH of the dialysate. The third medium is impregnated at a third region on the test strip. Alternatively, the test strip comprises a bicarbonate test pad attached to the first region, a glucose test pad attached to the second region, and optionally a pH test pad attached to the third region. The glucose test pad is impregnated with the first medium, the glucose test pad is impregnated with the second medium, and the pH test pad is impregnated with the third medium.

Abstract: A test strip for confirming a desired proportion of components in dialysate, including a first medium capable of indicating the concentration of bicarbonate ion, and a second medium capable of indicating the concentration of glucose. The test strip defines a first region impregnated with the first medium, and a second region impregnated with the second medium. The test strip optionally includes a third medium capable of indicating the pH of the dialysate. The third medium is impregnated at a third region on the test strip. Alternatively, the test strip comprises a bicarbonate test pad attached to the first region, a glucose test pad attached to the second region, and optionally a pH test pad attached to the third region. The glucose test pad is impregnated with the first medium, the glucose test pad is impregnated with the second medium, and the pH test pad is impregnated with the third medium.

Abstract: A test strip for confirming a desired proportion of components in dialysate, including a first medium capable of indicating the concentration of bicarbonate ion, and a second medium capable of indicating the concentration of glucose. The test strip defines a first region impregnated with the first medium, and a second region impregnated with the second medium. The test strip optionally includes a third medium capable of indicating the pH of the dialysate. The third medium is impregnated at a third region on the test strip. Alternatively, the test strip comprises a bicarbonate test pad attached to the first region, a glucose test pad attached to the second region, and optionally a pH test pad attached to the third region. The glucose test pad is impregnated with the first medium, the glucose test pad is impregnated with the second medium, and the pH test pad is impregnated with the third medium.

Abstract: A test strip for confirming a desired proportion of components in dialysate, including a first medium capable of indicating the concentration of bicarbonate ion, and a second medium capable of indicating the concentration of glucose. The test strip defines a first region impregnated with the first medium, and a second region impregnated with the second medium. The test strip optionally includes a third medium capable of indicating the pH of the dialysate. The third medium is impregnated at a third region on the test strip. Alternatively, the test strip comprises a bicarbonate test pad attached to the first region, a glucose test pad attached to the second region, and optionally a pH test pad attached to the third region. The glucose test pad is impregnated with the first medium, the glucose test pad is impregnated with the second medium, and the pH test pad is impregnated with the third medium.

Abstract: A test strip for confirming a desired proportion of components in dialysate, including a first medium capable of indicating the concentration of bicarbonate ion, and a second medium capable of indicating the concentration of glucose. The test strip defines a first region impregnated with the first medium, and a second region impregnated with the second medium. The test strip optionally includes a third medium capable of indicating the pH of the dialysate. The third medium is impregnated at a third region on the test strip. Alternatively, the test strip comprises a bicarbonate test pad attached to the first region, a glucose test pad attached to the second region, and optionally a pH test pad attached to the third region. The glucose test pad is impregnated with the first medium, the glucose test pad is impregnated with the second medium, and the pH test pad is impregnated with the third medium.

Abstract: A test strip for confirming a desired proportion of components in dialysate, including a first medium capable of indicating the concentration of bicarbonate ion, and a second medium capable of indicating the concentration of glucose. The test strip defines a first region impregnated with the first medium, and a second region impregnated with the second medium. The test strip optionally includes a third medium capable of indicating the pH of the dialysate. The third medium is impregnated at a third region on the test strip. Alternatively, the test strip comprises a bicarbonate test pad attached to the first region, a glucose test pad attached to the second region, and optionally a pH test pad attached to the third region. The glucose test pad is impregnated with the first medium, the glucose test pad is impregnated with the second medium, and the pH test pad is impregnated with the third medium.

Abstract: A test strip incubation device and method for developing a test strip having a reagent test pad disposed thereon, the reagent test pad requiring an incubation time, which allows greater accuracy and reliability of test results as well as a narrower window of PASS and FAIL indication concentrations. The device comprises a test strip holder which allows a wetted reagent test pad to be vertically stored in an enclosed reaction chamber during the incubation period. The reaction chamber is bounded by a substantially clear material to allow the user to easily observe the status of the reagent test pad held in the reaction chamber. The enclosed reaction chamber provides a high humidity environment for minimizing water evaporation from the reagent test pad during the incubation period. The device may be economically formed from low cost materials, is simple to use and facilitates disposal of the sample material after testing.

Abstract: A test strip incubation device and method for developing a test strip having a reagent test pad disposed thereon, the reagent test pad requiring an incubation time, which allows greater accuracy and reliability of test results as well as a narrower window of PASS and FAIL indication concentrations. The device comprises a test strip holder which allows a wetted reagent test pad to be vertically stored in an enclosed reaction chamber during the incubation period. The reaction chamber is bounded by a substantially clear material to allow the user to easily observe the status of the reagent test pad held in the reaction chamber. The enclosed reaction chamber provides a high humidity environment for minimizing water evaporation from the reagent test pad during the incubation period. The device may be economically formed from low cost materials, is simple to use and facilitates disposal of the sample material after testing.

Abstract: A test strip incubation device and method for developing a test strip having a reagent test pad disposed thereon, the reagent test pad requiring an incubation time, which allows greater accuracy and reliability of test results as well as a narrower window of PASS and FAIL indication concentrations. The device comprises a test strip holder which allows a wetted reagent test pad to be vertically stored in an enclosed reaction chamber during the incubation period. The reaction chamber is bounded by a substantially clear material to allow the user to easily observe the status of the reagent test pad held in the reaction chamber. The enclosed reaction chamber provides a high humidity environment for minimizing water evaporation from the reagent test pad during the incubation period. The device may be economically formed from low cost materials, is simple to use and facilitates disposal of the sample material after testing.

Abstract: A test strip incubation device and method for developing a test strip having a reagent test pad disposed thereon, the reagent test pad requiring an incubation time, which allows greater accuracy and reliability of test results as well as a narrower window of PASS and FAIL indication concentrations. The device comprises a test strip holder which allows a wetted reagent test pad to be vertically stored in an enclosed reaction chamber during the incubation period. The reaction chamber is bounded by a substantially clear material to allow the user to easily observe the status of the reagent test pad held in the reaction chamber. The enclosed reaction chamber provides a high humidity environment for minimizing water evaporation from the reagent test pad during the incubation period. The device may be economically formed from low cost materials, is simple to use and facilitates disposal of the sample material after testing.

Abstract: A unitized multilayer dry reagent analytical chemistry test structure and device and method of fabricating such device is described. The device includes two or more contiguous layers of absorbent or porous paper or polymeric material, at least one of which is incorporated with a test reagent composition, the layers attached to each other with intermediate porous attachment layers by fusion bonding. When the device is contacted with the fluid being tested the attachment layers allow the free flow of such fluid from one layer to the next.

Abstract: The reactant advantageously is an enzymatic reactant such as an enzyme substrate or coenzyme. The activity of the conjugated reactant as a constituent of a predetermined reaction is affected by reaction between the specific binding substance in the conjugate and a specific binding counterpart thereto. The presence of a ligand in a liquid medium may be determined using competitive or displacement binding or sequential saturation techniques wherein the specific binding substance in the conjugate is the ligand or a specific binding analog thereof, or using a direct binding technique wherein the specific binding substance is a specific binding partner of the ligand. The effect of the specific binding reaction on the activity of the conjugated reactant is related to the presence or amount of the ligand in the liquid medium tested.

Abstract: A test composition, device, and method for their use in a homogeneous specific binding assay which employs a substance having reactant activity, i.e., a reactant, as a labeling substance in the detection of a ligand in a liquid medium. The test composition and device comprise a conjugate formed of a specific binding substance coupled to the reactant. The reactant advantageously is an enzymatic reactant such as an enzyme substrate or coenzyme. The activity of the conjugated reactant as a constituent of a predetermined reaction is affected by reaction between the specific binding substance in the conjugate and a specific binding counterpart thereto. The presence of a ligand in a liquid medium may be determined using competitive or displacement binding or sequential saturation techniques wherein the specific binding substance in the conjugate is the ligand or a specific binding analog thereof, or using a direct binding technique wherein the specific binding substance is a specific binding partner of the ligand.

Abstract: An improved heterogenous specific binding assay method which employs a substance having reactant activity, i.e., a reactant, as a labeling substance in the detection of a ligand in a liquid medium. The method is carried out using reagent means which comprises, as its labeled constituent, a conjugate formed of a specific binding substance coupled to the reactant. The reactant advantageously is an enzymatic reactant such as an enzyme substrate or coenzyme. The activity of the conjugated reactant as a constituent of a predetermined reaction system is utilized as means for monitoring the extent of binding of the labeled constituent in conventional heterogenous specific binding assay schemes. The presence of a ligand in a liquid medium may be determined following conventional competitive binding manipulative techniques.

Abstract: A heterogeneous specific binding assay which employs a substance having reactant activity, i.e., a reactant, in a chemiluminescent reaction as a labeling substance in the detection of a ligand in a liquid medium. The method is carried out using reagent means which comprises, as its labeled constituent, a conjugate formed of a specific binding substance coupled to the chemiluminescent reactant. The activity of the conjugated reactant in the chemiluminescent reaction, i.e., the production of light, is utilized as means for monitoring the extent of binding of the labeled constituent in conventional heterogeneous specific binding assay schemes. The presence of a ligand in a liquid medium may be determined following conventional competitive binding manipulative techniques.

Abstract: Chemiluminescent-labeled conjugates of the formula: ##STR1## wherein one of R.sup.1 and R.sup.2 is hydrogen and the other is -NR.sup.3 R.sup.4 ; R.sup.3 is hydrogen or straight chain alkyl containing 1-4 carbon atoms and R.sup.4 is ##STR2## wherein n=1-3 and L(CO-- is the ligand or analog bound through an amide bond. Intermediates produced in the synthesis of such conjugates are also disclosed.

Abstract: Amino-functionalized phthalhydrazide intermediates of the formula: ##STR1## wherein one of R.sup.5 and R.sup.6 is hydrogen and the other is --NR.sup.7 R.sup.8 ; R.sup.7 is hydrogen or straight chain alkyl containing 1-4 carbon atoms and R.sup.8 is ##STR2## wherein n=1-3. The compounds are intermediates in the synthesis of chemiluminescent-labeled conjugates which are useful as reagents in specific binding assays for determining ligands or their specific binding partners in liquid media.

Abstract: An improved heterogenous specific binding assay method which employs a substance having reactant activity, i.e., a reactant, as a labeling substance in the detection of a ligand in a liquid medium. The method is carried out using reagent means which comprises, as its labeled constituent, a conjugate formed of a specific binding substance coupled to the reactant. The reactant advantageously is an enzymatic reactant such as an enzyme substrate or coenzyme. The activity of the conjugated reactant as a constituent of a predetermined reaction system is utilized as means for monitoring the extent of binding of the labeled constituent in conventional heterogenous specific binding assay schemes. The presence of a ligand in a liquid medium may be determined following conventional competitive binding manipulative techniques.

Abstract: An improved heterogeneous specific binding assay method which employs a substance having reactant activity, i.e., a reactant, as a labeling substance in the detection of a ligand in a liquid medium. The method is carried out using reagent means which comprises, as its labeled constituent, a conjugate formed of a specific binding substance coupled to the reactant. The reactant advantageously is an enzymatic reactant such as an enzyme substrate or coenzyme. The activity of the conjugated reactant as a constituent of a predetermined reaction system is utilized as means for monitoring the extent of binding of the labeled constituent in conventional heterogeneous specific binding assay schemes. The presence of a ligand in a liquid medium may be determined following conventional competitive binding manipulative techniques.

Abstract: A specific binding assay method employing, as a labeling substance, a reversibly binding enzyme modulator for the detection of a ligand in a liquid medium. The method follows conventional specific binding assay techniques of either the homogeneous or heterogeneous type wherein the liquid medium to be assayed is combined with reagent means that includes a labeled conjugate to form a binding reaction system having a bound-species and a free-species of the conjugate. The amount of conjugate resulting in the bound-species or the free-species is a function of the amount of ligand present in the liquid medium assayed. In the present invention, the labeled conjugate comprises a reversibly binding enzyme modulator covalently linked to a binding component of the binding reaction system.