Patents by Inventor James E. Dahlberg

James E. Dahlberg has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Synthesis-deficient thermostable DNA polymerase
    Patent number: 5795763
    Abstract: A means for cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is employed as the basis of a novel method of detection of specific nucleic acid sequences.
    Type: Grant
    Filed: June 6, 1995
    Date of Patent: August 18, 1998
    Assignee: Third Wave Technologies, Inc.
    Inventors: James E. Dahlberg, Victor I. Lyamichev, Mary Ann D. Brow
  • Cleavase fragment length polymorphism
    Patent number: 5719028
    Abstract: A means for cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is employed as the basis of a novel method of detection of specific nucleic acid sequences.
    Type: Grant
    Filed: February 6, 1997
    Date of Patent: February 17, 1998
    Assignee: Third Wave Technologies Inc.
    Inventors: James E. Dahlberg, Victor I. Lyamichev, Mary Ann D. Brow, Mary C. Oldenburg
  • Detection of target nucleic acid molecules using synthesis-deficient thermostable DNA polymerase
    Patent number: 5691142
    Abstract: A means for cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is employed as the basis of a novel method of detection of specific nucleic acid sequences. In this novel detection method, the signal indicative of the presence of the target nucleic acid sequence is amplified through cleavage of detection molecule(s) which comprise hairpin structures.
    Type: Grant
    Filed: June 6, 1995
    Date of Patent: November 25, 1997
    Assignee: Third WaveTechnologies, Inc.
    Inventors: James E. Dahlberg, Victor I. Lyamichev, Mary Ann D. Brow
  • 5' nucleases derived from thermostable DNA polymerase
    Patent number: 5614402
    Abstract: A means cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is employed as the basis of a novel method of detection of specific nucleic acid sequences.
    Type: Grant
    Filed: June 6, 1994
    Date of Patent: March 25, 1997
    Assignee: Third Wave Technologies, Inc.
    Inventors: James E. Dahlberg, Victor I. Lyamichev, Mary Ann D. Brow
  • Nucleic acid encoding synthesis-deficient thermostable DNA polymerase
    Patent number: 5541311
    Abstract: A means for cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is employed as the basis of a novel method of detection of specific nucleic acid sequences. Cleaving enzymes are produced through the use of novel DNA sequences which encode novel thermostable polymerases.
    Type: Grant
    Filed: June 4, 1993
    Date of Patent: July 30, 1996
    Assignee: Third Wave Technologies, Inc.
    Inventors: James E. Dahlberg, Victor I. Lyamichev, Mary Ann D. Brow
  • Method of site specific nucleic acid cleavage
    Patent number: 5422253
    Abstract: A method of cleaving a target nucleic acid molecule is disclosed. A cleavage structure is formed that comprises the target nucleic acid and a pilot nucleic acid. A first region of the target nucleic acid is annealed to the pilot nucleic acid to form a duplex structure. A second region of the target nucleic acid contiguous to the duplex is not annealed to the pilot nucleic acid, thus forming a junction site between the duplex region and the non-annealed region. The cleavage structure is exposed to a cleavage agent capable of preferentially cleaving the cleavage structure at a target site in a manner independent of the sequence of the cleavage structure. The cleavage structure and the cleavage agent are incubated under conditions wherein cleavage can occur.
    Type: Grant
    Filed: December 7, 1992
    Date of Patent: June 6, 1995
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: James E. Dahlberg, Victor I. Lyamichev, Mary A. D. Brow