Patents by Inventor James E. Fleming
James E. Fleming has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8748159Abstract: A rapid method for the quantitation of various live cell types is described. This new cell fluorescence method correlates with other methods of enumerating cells such as the standard plate count, the methylene blue method and the slide viability technique. The method is particularly useful in several applications such as: a) quantitating bacteria in milk, yogurt, cheese, meat and other foods, b) quantitating yeast cells in brewing, fermentation and bread making, c) quantitating mammalian cells in research, food and clinical settings. The method is especially useful when both total and viable cell counts are required such as in the brewing industry. The method can also be employed to determine the metabolic activity of cells in a sample. The apparatus, device, and/or system used for cell quantitation is also disclosed.Type: GrantFiled: October 28, 2011Date of Patent: June 10, 2014Assignee: GenPrime, Inc.Inventors: James E. Fleming, Jason Buck Somes, Darby McLean, Jerad R. Holcomb
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Patent number: 8698881Abstract: An assay system includes an optical imager to acquire high resolution images of assay strips (e.g., lateral flow immunochromatographic test strips) and performs image processing to identify individual assay strips and determine results for each assay strip, by quantifies the presence or absence of test signal line(s) and control signal line(s). Assay strips may be in a holder or carrier contained in a specimen container also holding a specimen. The assay system automatically logs all results and data to a database that stores a high resolution image of the original immunochromatographic assay, the values of test line(s) and control line(s), and the test result. A user interface directs an end user through operation.Type: GrantFiled: March 5, 2013Date of Patent: April 15, 2014Assignee: GenPrime, IncInventors: James E Fleming, Jesse M. McGrew, Stephen P. McGrew, Patrick Nicholes, Johnny Humphreys
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Patent number: 8446463Abstract: An assay system includes an optical imager to acquire high resolution images of assay strips (e.g., lateral flow immunochromatographic test strips) and performs image processing to identify individual assay strips and determine results for each assay strip, by quantifies the presence or absence of test signal line(s) and control signal line(s). Assay strips may be in a holder or carrier contained in a specimen container also holding a specimen. The assay system automatically logs all results and data to a database that stores a high resolution image of the original immunochromatographic assay, the values of test line(s) and control line(s), and the test result. A user interface directs an end user through operation.Type: GrantFiled: August 10, 2009Date of Patent: May 21, 2013Assignee: GenPrime, Inc.Inventors: James E. Fleming, Jesse M. McGrew, Stephen P. McGrew, Patrick Nicholes, Johnny Humphreys
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Publication number: 20120164677Abstract: A rapid method for the quantitation of various live cell types is described. This new cell fluorescence method correlates with other methods of enumerating cells such as the standard plate count, the methylene blue method and the slide viability technique. The method is particularly useful in several applications such as: a) quantitating bacteria in milk, yogurt, cheese, meat and other foods, b) quantitating yeast cells in brewing, fermentation and bread making, c) quantitating mammalian cells in research, food and clinical settings. The method is especially useful when both total and viable cell counts are required such as in the brewing industry. The method can also be employed to determine the metabolic activity of cells in a sample. The apparatus, device, and/or system used for cell quantitation is also disclosed.Type: ApplicationFiled: October 28, 2011Publication date: June 28, 2012Applicant: GENPRIME, INC.Inventors: James E. Fleming, Jason Buck Somes, Darby McLean, Jerad R. Holcomb
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Patent number: 8071326Abstract: A rapid method for the quantitation of various live cell types is described. This new cell fluorescence method correlates with other methods of enumerating cells such as the standard plate count, the methylene blue method and the slide viability technique. The method is particularly useful in several applications such as: a) quantitating bacteria in milk, yogurt, cheese, meat and other foods, b) quantitating yeast cells in brewing, fermentation and bread making, c) quantitating mammalian cells in research, food and clinical settings. The method is especially useful when both total and viable cell counts are required such as in the brewing industry. The method can also be employed to determine the metabolic activity of cells in a sample. The apparatus, device, and/or system used for cell quantitation is also disclosed.Type: GrantFiled: February 11, 2011Date of Patent: December 6, 2011Assignee: Genprime, Inc.Inventors: James E. Fleming, Jason Buck Somes, Darby McLean, Jerad R. Holcomb
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Publication number: 20110136159Abstract: A rapid method for the quantitation of various live cell types is described. This new cell fluorescence method correlates with other methods of enumerating cells such as the standard plate count, the methylene blue method and the slide viability technique. The method is particularly useful in several applications such as: a) quantitating bacteria in milk, yogurt, cheese, meat and other foods, b) quantitating yeast cells in brewing, fermentation and bread making, c) quantitating mammalian cells in research, food and clinical settings. The method is especially useful when both total and viable cell counts are required such as in the brewing industry. The method can also be employed to determine the metabolic activity of cells in a sample. The apparatus, device, and/or system used for cell quantitation is also disclosed.Type: ApplicationFiled: February 11, 2011Publication date: June 9, 2011Applicant: GenPrime, Inc.Inventors: James E. Fleming, Jason Buck Somes, Darby McLean, Jerad R. Holcomb
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Publication number: 20110076706Abstract: The present invention provides analytical methods and related kits for detecting the presence of bacteria in a sample, determining the amount of bacteria in a sample, or determining the type of bacteria in a sample. In particular, the invention relates to devices and methods suitable for the rapid detection, quantification, or identification of bacteria in a liquid sample by measuring bacterial ATP activity.Type: ApplicationFiled: June 25, 2010Publication date: March 31, 2011Applicant: GENPRIME, INC.Inventors: James E. Fleming, Claire Norton, Cheryl Fleming
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Patent number: 7906295Abstract: A rapid method for the quantitation of various live cell types is described. This new cell fluorescence method correlates with other methods of enumerating cells such as the standard plate count, the methylene blue method and the slide viability technique. The method is particularly useful in several applications such as: a) quantitating bacteria in milk, yogurt, cheese, meat and other foods, b) quantitating yeast cells in brewing, fermentation and bread making, c) quantitating mammalian cells in research, food and clinical settings. The method is especially useful when both total and viable cell counts are required such as in the brewing industry. The method can also be employed to determine the metabolic activity of cells in a sample. The apparatus, device, and/or system used for cell quantitation is also disclosed.Type: GrantFiled: December 19, 2008Date of Patent: March 15, 2011Assignee: GenPrime, Inc.Inventors: James E. Fleming, Jason Buck Somes, Darby McLean, Jerad R. Holcomb
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Publication number: 20100045789Abstract: An assay system includes an optical imager to acquire high resolution images of assay strips (e.g., lateral flow immunochromatographic test strips) and performs image processing to identify individual assay strips and determine results for each assay strip, by quantifies the presence or absence of test signal line(s) and control signal line(s). Assay strips may be in a holder or carrier contained in a specimen container also holding a specimen. The assay system automatically logs all results and data to a database that stores a high resolution image of the original immunochromatographic assay, the values of test line(s) and control line(s), and the test result. A user interface directs an end user through operation.Type: ApplicationFiled: August 10, 2009Publication date: February 25, 2010Inventors: James E. Fleming, Jesse M. McGrew, Stephen P. McGrew, Patrick Nicholes, Johnny Humphreys
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Patent number: 7527924Abstract: A rapid method for the quantitation of various live cell types is described. The method may include a variety of steps including: 1) suspending the cells in a detergent-like compound, 2) isolating the washed cells by centrifugation or filtration, 3) resuspending the cells in a solution that contains a preservative, a fluorescent dye and a compound such as dequalinium which can be taken up by the cells, 4) measuring the fluorescence increase over time of the cell-dye mixture with a simple fluorometer, and 5) measuring the native fluorescence of the cells. This new cell fluorescence method correlates with other methods of enumerating cells such as the standard plate count, the methylene blue method and the slide viability technique. The method is particularly useful in several applications such as: a) quantitating bacteria in milk, yogurt, cheese, meat and other foods, b) quantitating yeast cells in brewing, fermentation and bread making, c) quantitating mammalian cells in research, food and clinical settings.Type: GrantFiled: June 2, 2005Date of Patent: May 5, 2009Assignee: GenPrime, Inc.Inventors: James E Fleming, Jason Buck Somes, Darby McLean, Jerad R Holcomb
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Publication number: 20090104652Abstract: A rapid method for the quantitation of various live cell types is described. This new cell fluorescence method correlates with other methods of enumerating cells such as the standard plate count, the methylene blue method and the slide viability technique. The method is particularly useful in several applications such as: a) quantitating bacteria in milk, yogurt, cheese, meat and other foods, b) quantitating yeast cells in brewing, fermentation and bread making, c) quantitating mammalian cells in research, food and clinical settings. The method is especially useful when both total and viable cell counts are required such as in the brewing industry. The method can also be employed to determine the metabolic activity of cells in a sample. The apparatus, device, and/or system used for cell quantitation is also disclosed.Type: ApplicationFiled: December 19, 2008Publication date: April 23, 2009Applicant: GENPRIME, INC.Inventors: James E. Fleming, Jason Buck Somes, Darby McLean, Jerad R. Holcomb
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Patent number: 7491488Abstract: The present invention includes compositions, kits, and methods useful for the detection of bacteria. These agents and methods are primarily directed to a method of detecting the presence of bacteria in a sample, involving incubating the sample with an agent that binds to bacteria, such as, e.g., an agent specific for peptidoglycan or a component thereof, and then detecting bound bacteria. The invention includes lateral-flow immunoassay methods and devices for assessing the total bacterial load in a liquid sample.Type: GrantFiled: June 9, 2005Date of Patent: February 17, 2009Assignee: GenPrime, Inc.Inventors: James E. Fleming, Jason Buck Somes
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Publication number: 20080153114Abstract: The present invention includes compositions, kits, and methods useful for the detection of bacteria. These agents and methods are primarily directed to a method of detecting the presence of bacteria in a sample, involving incubating the sample with an agent that binds to bacteria, such as, e.g., an agent specific for peptidoglycan or a component thereof, and then detecting bound bacteria. The invention includes lateral-flow immunoassay methods and devices for assessing the total bacterial load in a liquid sample.Type: ApplicationFiled: March 7, 2008Publication date: June 26, 2008Applicant: GENPRIME, INC.Inventors: James E. Fleming, Jason Buck Somes
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Patent number: 6787302Abstract: This invention describes methods and kits for detecting and quantifying viable cells in a sample using fluorescent dyes that can be internalized predominantly by viable cells and have fluorescent properties measurably altered when bound to target components. These methods and kits provide a rapid and cost-effective means of detecting potential biological threats in the field.Type: GrantFiled: March 1, 2002Date of Patent: September 7, 2004Assignee: GenPrime, Inc.Inventors: James E. Fleming, Jason Buck Somes
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Patent number: 6673568Abstract: This invention describes kits for quantifying viable cells in a sample using fluorescent dyes that can be internalized predominately by viable cells and have fluorescence properties measurably altered when bound to target components. These kits circumvent the need for training personnel in plating, growing and count viable cells, and reduce both the time and the cost required for cellular quantitation according to existing techniques.Type: GrantFiled: October 24, 2000Date of Patent: January 6, 2004Assignee: Genprime, Inc.Inventors: James E. Fleming, Jason Buck Somes
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Publication number: 20020168630Abstract: This invention describes methods and kits for detecting and quantifying viable cells in a sample using fluorescent dyes that can be internalized predominantly by viable cells and have fluorescent properties measurably altered when bound to target components. These methods and kits provide a rapid and cost-effective means of detecting potential biological threats in the field.Type: ApplicationFiled: March 1, 2002Publication date: November 14, 2002Inventors: James E. Fleming, Jason Buck Somes
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Publication number: 20020055134Abstract: A rapid method for the quantitation of various live cell types is described. The method may include a variety of steps including: 1) suspending the cells in a detergent-like compound, 2) isolating the washed cells by centrifugation or filtration, 3) resuspending the cells in a solution that contains a preservative, a fluorescent dye and a compound such as dequalinium which can be taken up by the cells, 4) measuring the fluorescence increase over time of the cell-dye mixture with a simple fluorometer, and 5) measuring the native fluorescence of the cells. This new cell fluorescence method correlates with other methods of enumerating cells such as the standard plate count, the methylene blue method and the slide viability technique. The method is particularly usefull in several applications such as: a) quantitating bacteria in milk, yogurt, cheese, meat and other foods, b) quantitating yeast cells in brewing, fermentation and bread making, c) quantitating mammalian cells in research, food and clinical settings.Type: ApplicationFiled: July 24, 2001Publication date: May 9, 2002Inventors: James E. Fleming, Jason Buck Somes, Darby McLean, Jerad R. Holcomb
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Patent number: 5101020Abstract: Novel radio labeled dyes are provided for detecting proteins on gels.Type: GrantFiled: August 8, 1990Date of Patent: March 31, 1992Assignee: Linus Pauling Institute of Science and MedicineInventor: James E. Fleming
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Patent number: 4966854Abstract: Novel radio labelled dyes are provided for detecting proteins on gels.Type: GrantFiled: May 12, 1988Date of Patent: October 30, 1990Assignee: Linus Pauling Institute of Science and MedicineInventor: James E. Fleming
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Patent number: 4769334Abstract: A method is provided for detecting proteins on gels utilizing .sup.14 C-labeled dyes.Type: GrantFiled: February 12, 1986Date of Patent: September 6, 1988Assignee: Linus Pauling Institute of Science and MedicineInventor: James E. Fleming