Patents by Inventor James E. Haber

James E. Haber has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Multiplex production and barcoding of genetically engineered cells
    Patent number: 12416015
    Abstract: The present disclosure relates to multiplex production and phenotyping of genetically engineered cells using RNA-guided nucleases and genomic barcoding. In particular, high-throughput multiplex genome editing is achieved utilizing a system that facilitates precise genome editing at desired target chromosomal loci by homology directed repair. Integration of guide RNA and donor DNA sequences as a genomic barcode at a separate chromosomal locus allows identification, isolation, and massively-parallel validation of individual variants from a pool of transformants. Strains can be arrayed according to their precise genetic modifications, as specified by donor DNA incorporation in heterologous or native genes.
    Type: Grant
    Filed: September 14, 2018
    Date of Patent: September 16, 2025
    Assignees: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY, BRANDEIS UNIVERSITY
    Inventors: Kevin Roy, Justin D. Smith, Robert P. St. Onge, Lars M. Steinmetz, James E. Haber
  • SITE-SPECIFIC CLEAVAGE AND ELIMINATION OF DNA IN BACTERIAL SPECIES WITH SEGMENTED CHROMOSOMES
    Publication number: 20240226247
    Abstract: Described herein is a method of reducing virulence of a bacterial species with a segmented genome which has infected a mammalian host or mammalian host cell, by exposing the bacterial genome to an RNA-guided nuclease and a guide RNA (gRNA), thus generating a double-stranded or single-stranded break in the bacterial genome and causing loss of the targeted genome segment.
    Type: Application
    Filed: September 19, 2023
    Publication date: July 11, 2024
    Inventors: Yuko Nakajima, James E. Haber, Christine Jacobs-Wagner, Constantin N. Takacs
  • SITE-SPECIFIC CLEAVAGE AND ELIMINATION OF DNA IN BACTERIAL SPECIES WITH SEGMENTED CHROMOSOMES
    Publication number: 20240131122
    Abstract: Described herein is a method of reducing virulence of a bacterial species with a segmented genome which has infected a mammalian host or mammalian host cell, by exposing the bacterial genome to an RNA-guided nuclease and a guide RNA (gRNA), thus generating a double-stranded or single-stranded break in the bacterial genome and causing loss of the targeted genome segment.
    Type: Application
    Filed: September 18, 2023
    Publication date: April 25, 2024
    Inventors: Yuko Nakajima, James E. Haber, Christine Jacobs-Wagner, Constantin N. Takacs
  • MULTIPLEX PRODUCTION AND BARCODING OF GENETICALLY ENGINEERED CELLS
    Publication number: 20200270632
    Abstract: The present disclosure relates to multiplex production and phenotyping of genetically engineered cells using RNA-guided nucleases and genomic barcoding. In particular, high-throughput multiplex genome editing is achieved utilizing a system that facilitates precise genome editing at desired target chromosomal loci by homology directed repair. Integration of guide RNA and donor DNA sequences as a genomic barcode at a separate chromosomal locus allows identification, isolation, and massively-parallel validation of individual variants from a pool of transformants. Strains can be arrayed according to their precise genetic modifications, as specified by donor DNA incorporation in heterologous or native genes.
    Type: Application
    Filed: September 14, 2018
    Publication date: August 27, 2020
    Inventors: Kevin Roy, Justin D. Smith, Robert P. St. Onge, Lars M. Steinmetz, James E. Haber