Patents by Inventor James F. Zawada

James F. Zawada has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Mono charging system for selectively introducing non-native amino acids into proteins using an in vitro protein synthesis system
    Patent number: 8778631
    Abstract: This invention provides for a novel means of incorporating non-native amino acids into preselected positions of a protein using a cell-free synthesis system. The methods involve the use of non-orthogonal, native isoaccepting sense tRNAs that are encoded by the genetic code. Such methods allow for numerous non-native amino acids to be incorporated through the use of sense codons without having to rely upon orthogonal tRNA-synthetase pairs.
    Type: Grant
    Filed: January 12, 2010
    Date of Patent: July 15, 2014
    Assignee: Sutro Biopharma, Inc.
    Inventors: Alexei M. Voloshin, James F. Zawada, Daniel Gold, Christopher James Murray, James Edward Rozzelle, Nathan Uter, Gang Yin
  • USE OF DNA GYRASE INHIBITORS FOR IN VITRO POLYPEPTIDE SYNTHESIS REACTIONS
    Publication number: 20100203587
    Abstract: The present invention provides methods and compositions useful for in vitro polypeptide synthesis reactions. The methods involve the use of DNA gyrase inhibitors to prevent bacterial contamination in lysates used for in vitro production of polypeptides. The compositions include contamination-free cell lysates for in vitro protein synthesis reactions.
    Type: Application
    Filed: January 12, 2010
    Publication date: August 12, 2010
    Applicant: Sutro Biopharma, Inc.
    Inventors: Alexei M. Voloshin, James F. Zawada, Daniel Gold
  • DUAL CHARGING SYSTEM FOR SELECTIVELY INTRODUCING NON-NATIVE AMINO ACIDS INTO PROTEINS USING AN IN VITRO SYNTHESIS METHOD
    Publication number: 20100184134
    Abstract: This invention provides for a novel means of incorporating non-native amino acids into preselected positions of a protein using a cell-free synthesis system. The methods involve the use of non-orthogonal, native isoaccepting sense tRNAs that are encoded by the genetic code. Such methods allow for numerous non-native amino acids to be incorporated through the use of sense codons without having to rely upon orthogonal tRNA-synthetase pairs.
    Type: Application
    Filed: January 11, 2010
    Publication date: July 22, 2010
    Applicant: Sutro Biopharma, Inc.
    Inventors: Alexei M. Voloshin, James F. Zawada, Daniel Gold, Christopher James Murray, James Edward Rozzelle, Nathan Uter, Gang Yin
  • MONO CHARGING SYSTEM FOR SELECTIVELY INTRODUCING NON-NATIVE AMINO ACIDS INTO PROTEINS USING AN IN VITRO PROTEIN SYNTHESIS SYSTEM
    Publication number: 20100184135
    Abstract: This invention provides for a novel means of incorporating non-native amino acids into preselected positions of a protein using a cell-free synthesis system. The methods involve the use of non-orthogonal, native isoaccepting sense tRNAs that are encoded by the genetic code. Such methods allow for numerous non-native amino acids to be incorporated through the use of sense codons without having to rely upon orthogonal tRNA-synthetase pairs.
    Type: Application
    Filed: January 12, 2010
    Publication date: July 22, 2010
    Applicant: Sutro Biopharma, Inc.
    Inventors: Alexei M. Voloshin, James F. Zawada, Daniel Gold, Christopher James Murray, James Edward Rozzelle, Nathan Uter, Gang Yin