Abstract: The present disclosure describes a system and methods for positioning a configured number of biomaterials on a surface. The method disclosed includes the formation of a membrane mask structure including a configured number of nanopores. The nanopores are generally sized to be the same size as, or slightly larger than a single target biomaterial. The masking membrane is then adhered to a substrate. The substrate may include a raw substrate, or may be functionalized for the purpose of binding biomaterials with greater attraction. A solution including the target biomaterials is then exposed to the masking membrane. One biomaterial is able to adhere to the substrate through each nanopore. The solution is rinsed from the membrane surface leaving only the biomaterial that has adhered to the substrate with a relatively strong binding force. The masking membrane may then either remain or may be removed.

Abstract: A microfluidic system includes a hydrophobic fluidic platform and a heater. The platform includes a plurality of electrode cells operably connected to a voltage source and a controller. The heater is configured to fuse first and second vesicles. The first and second vesicles encapsulate first and second DNA precursors, respectively. The fusing combines the first and second DNA precursors. In another embodiment, a microfluidic system includes a fluidic platform including a plurality of electrode cells, a vesicle mover, and a reaction facilitator. The vesicle mover is configured to move first and second vesicles to a selected cell of the plurality of electrode cells. The reaction facilitator is operably connected to the selected cell. A method includes providing a fluidic platform comprising a plurality of cells; moving first and second vesicles encapsulating first and second reagents, respectively, to a first cell; and fusing the first and second vesicles.

Abstract: A light detection and ranging system can have a light source coupled to a polygon reflector having a plurality of facets. A controller can be connected to the light source and directed to generate and execute a calibration strategy that alters an orientation of the light source in response to identification of a position of at least one facet of the plurality of facets.