Abstract: Provided are an esterase mutant and use thereof. The amino acid sequence of the esterase mutant has a sequence as shown in SEQ ID NO: 1, and sites at which amino acid mutations occur include an N51G site.

Abstract: Provided are an amino acid dehydrogenase mutant and use thereof. The amino acid sequence of the amino acid dehydrogenase mutant has a sequence as shown in SEQ ID NO: 1, and sites at which amino acid mutations occur include a K144G site.

Abstract: Provided are a lipase mutant and use thereof. The amino acid sequence of the lipase mutant has a sequence shown in SEQ ID NO: 1, that is, sites at which amino acid mutations occur include a V154L site.

Abstract: The present application relates to the technical field of genetic engineering, and provides a monooxygenase mutant, a preparation method and application thereof. The monooxygenase mutant has any one of the amino acid sequences shown in (I) and (II): (I) an amino acid sequence having at least 80% identity with the amino acid sequence shown in SEQ ID NO. 1; and (II) an amino acid sequence obtained by modifying, substituting, deleting, or adding one or several amino acids to the amino acids at 23 to 508 positions of the amino acid sequence shown in SEQ ID NO. 1, the substituting referring to a substitution of 1 to 34 amino acids, wherein the mutant has the activity of monooxygenase.

Abstract: Provided is a ketoreductase mutant and a method for producing chiral alcohol using the same. The ketoreductase mutant has a sequence with amino acid mutations in the sequence shown in SEQ ID NO:1. The mutation sites include at least one of the following positions: 6th position, 21st position, 42nd position, 58th position, 61st position, 76th position, 87th position, 94th position, 96th position, 108th position, 113th position, 117th position, 144th position, 146th position, 147th position, 149th position, 151st position, 152nd, 156th position, 165th position, 177th position, and 198th position.

Abstract: A thermal welding marking system for attachment to a thermal welding machine including: a hammer arm rotatably connected to a mounting bracket attached to the thermal welding machine, wherein a distal end of the hammer arm is shaped to receive a marker or pen therein; a cable or spring connected to the distal end of the hammer arm; a cable connecting the hammer arm to a hand lever mounted adjacent to hand controls on the thermal welding machine, wherein movement of the hand lever raises or lowers the distal end of the hammer arm and thereby raises or lowers the marker or pen therein. In operation, the thermal welding machine is advanced across the edges of overlapping building roofing material and the marker or pen is intermittently lowered/raised to mark locations along a seam of the edges of overlapping building roofing material where the seam is either open or closed.

Abstract: The present application relates to the technical field of genetic engineering, and provides a monooxygenase mutant, a preparation method and application thereof. The monooxygenase mutant has any one of the amino acid sequences shown in (I) and (II): (I) an amino acid sequence having at least 80% identity with the amino acid sequence shown in SEQ ID NO. 1; and (II) an amino acid sequence obtained by modifying, substituting, deleting, or adding one or several amino acids to the amino acids at 23 to 508 positions of the amino acid sequence shown in SEQ ID NO. 1, the substituting referring to a substitution of 1 to 34 amino acids, wherein the mutant has the activity of monooxygenase.

Abstract: A thermal welding marking system for attachment to a thermal welding machine including: a hammer arm rotatably connected to a mounting bracket attached to the thermal welding machine, wherein a distal end of the hammer arm is shaped to receive a marker or pen therein; a cable or spring connected to the distal end of the hammer arm; a cable connecting the hammer arm to a hand lever mounted adjacent to hand controls on the thermal welding machine, wherein movement of the hand lever raises or lowers the distal end of the hammer arm and thereby raises or lowers the marker or pen therein. In operation, the thermal welding machine is advanced across the edges of overlapping building roofing material and the marker or pen is intermittently lowered/raised to mark locations along a seam of the edges of overlapping building roofing material where the seam is either open or closed.

Abstract: The application provides a Diketoreductase (DKR) mutant, its nucleotide coding sequence, and an expression cassette, recombinant vector and host cell containing the sequence, as well as a method for application of the mutant to the preparation of 3R,5S-dicarbonyl compound. An ee value of the obtained 3R,5S-dicarbonyl compound is higher than 99%, and a de value is about 90%. The DKR mutant is a key pharmaceutical intermediate, and particularly provides an efficient catalyst for synthesis of a chiral dicarbonyl hexanoic acid chain of a statin drug.

Abstract: Provided are an intermediate compound for preparing rosuvastatin calcium and a preparation method of the rosuvastatin calcium. The method comprises: using the foregoing intermediate compound as a raw material, and subjecting the raw material to a step of Wittig reaction, a step of protecting group removal and hydrolysis and a step of calcium salt formation, so as to obtain the rosuvastatin calcium. The product, which is prepared from the intermediate compound, can be substantially enhanced in stereoselectivity and also notably improved in purity and yield; in addition, the method for preparing rosuvastatin calcium from the intermediate compound is simple, convenient and low in cost.

Abstract: The application provides a Diketoreductase (DKR) mutant, its nucleotide coding sequence, and an expression cassette, recombinant vector and host cell containing the sequence, as well as a method for application of the mutant to the preparation of 3R,5S-dicarbonyl compound. An ee value of the obtained 3R,5S-dicarbonyl compound is higher than 99%, and a de value is about 90%. The DKR mutant is a key pharmaceutical intermediate, and particularly provides an efficient catalyst for synthesis of a chiral dicarbonyl hexanoic acid chain of a statin drug.

Abstract: Provided are an intermediate compound for preparing rosuvastatin calcium and a preparation method of the rosuvastatin calcium. The method comprises: using the foregoing intermediate compound as a raw material, and subjecting the raw material to a step of Wittig reaction, a step of protecting group removal and hydrolysis and a step of calcium salt formation, so as to obtain the rosuvastatin calcium. The product, which is prepared from the intermediate compound, can be substantially enhanced in stereoselectivity and also notably improved in purity and yield; in addition, the method for preparing rosuvastatin calcium from the intermediate compound is simple, convenient and low in cost.

Abstract: A pallet picking adapter (100) is adapted for use with a forklift truck (102). The pallet picking adapter (100) includes a bulkhead (130) attached to a frame of the forklift truck (102). The adapter (100) further comprises a deck system (178) having a pair of deck assemblies (180). The deck assemblies (180) can be stored with the bulkhead (130) in the vertical, upright position. For use, the deck assemblies (180) can be individually lowered so as to rest upon forks (120, 122) of the truck (102). In these operable positions, the deck assemblies (180) can be utilized to transport CooLift pallets (320, 322). When the deck assemblies (180) are in the stored, upright position, the adapter 100 can be utilized to transport conventional pallets of standards sizes.

Abstract: Disclosed is a method for synthesizing sapropterin dihydrochloride. The present disclosure reduces a synthesis route of the sapropterin dihydrochloride, and resolves a racemate intermediate or an intermediate having a low antimer isomerism value by using a chiral resolving reagent, thereby obtaining an intermediate having a high antimer isomerism value. Raw materials are cheap and readily available, and the cost is significantly reduced, hence providing an effective scheme for mass industrial production of the sapropterin dihydrochloride.

Abstract: Disclosed is a method for synthesizing sapropterin dihydrochloride. The present disclosure reduces a synthesis route of the sapropterin dihydrochloride, introduces a chiral center in an asymmetric synthesis manner, in which a tetrahydrofuran solution containing a samarium catalyst is adopted as a catalyst, and obtains a target compound having a high antimer isomerism value by means of selective catalysis. The yield is improved, raw materials are cheap and readily available, and the cost is significantly reduced, hence providing an effective scheme for mass industrial production of the sapropterin dihydrochloride.

Abstract: A pallet picking adapter (100) is adapted for use with a forklift truck (102). The pallet picking adapter (100) includes a bulkhead (130) attached to a frame of the forklift truck (102). The adapter (100) further comprises a deck system (178) having a pair of deck assemblies (180). The deck assemblies (180) can be stored with the bulkhead (130) in the vertical, upright position. For use, the deck assemblies (180) can be individually lowered so as to rest upon forks (120, 122) of the truck (102). In these operable positions, the deck assemblies (180) can be utilized to transport CooLift pallets (320, 322). When the deck assemblies (180) are in the stored, upright position, the adapter 100 can be utilized to transport conventional pallets of standards sizes.

Abstract: Disclosed is a method for synthesizing sapropterin dihydrochloride. The present disclosure reduces a synthesis route of the sapropterin dihydrochloride, and resolves a racemate intermediate or an intermediate having a low antimer isomerism value by using a chiral resolving reagent, thereby obtaining an intermediate having a high antimer isomerism value. Raw materials are cheap and readily available, and the cost is significantly reduced, hence providing an effective scheme for mass industrial production of the sapropterin dihydrochloride.

Abstract: Disclosed is a method for synthesizing sapropterin dihydrochloride. The present disclosure reduces a synthesis route of the sapropterin dihydrochloride, introduces a chiral center in an asymmetric synthesis manner, in which a tetrahydrofuran solution containing a samarium catalyst is adopted as a catalyst, and obtains a target compound having a high antimer isomerism value by means of selective catalysis. The yield is improved, raw materials are cheap and readily available, and the cost is significantly reduced, hence providing an effective scheme for mass industrial production of the sapropterin dihydrochloride.