Patents by Inventor James Ghadiali
James Ghadiali has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230348975Abstract: Disclosed herein include systems, methods, compositions, and kits for determining protein expression and gene expression simultaneously and for sample indexing. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) comprises one or more of a unique molecular label sequence, a primer adapter, antibody-specific barcode sequence, an alignment sequence, and/or a poly(A) sequence. In some embodiments, the oligonucleotide is associated with the cellular component-binding reagent via a linker (e.g., 5AmMC12).Type: ApplicationFiled: March 21, 2023Publication date: November 2, 2023Inventors: Eleen Shum, Katherine Lazaruk, Dennis E. Prosen, Devon Jensen, David Rosenfeld, Janice H. Lai, James Ghadiali, Christina Chang
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Patent number: 11661631Abstract: Disclosed herein include systems, methods, compositions, and kits for determining protein expression and gene expression simultaneously and for sample indexing. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) comprises one or more of a unique molecular label sequence, a primer adapter, antibody-specific barcode sequence, an alignment sequence, and/or a poly(A) sequence. In some embodiments, the oligonucleotide is associated with the cellular component-binding reagent via a linker (e.g., 5AmMC12).Type: GrantFiled: January 21, 2020Date of Patent: May 30, 2023Assignee: Becton, Dickinson and CompanyInventors: Eleen Shum, Katherine Lazaruk, Dennis E. Prosen, Devon Jensen, David Rosenfeld, Janice H. Lai, James Ghadiali, Christina Chang
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Publication number: 20230125113Abstract: Some embodiments disclosed herein provide a plurality of compositions each comprising a protein binding reagent conjugated with an oligonucleotide. The oligonucleotide comprises a unique identifier for the protein binding reagent it is conjugated with, and the protein binding reagent is capable of specifically binding to a protein target. Further disclosed are methods and kits for quantitative analysis of a plurality of protein targets in a sample and for simultaneous quantitative analysis of protein and nucleic acid targets in a sample. Also disclosed herein are systems and methods for preparing a labeled biomolecule reagent, including a labeled biomolecule agent comprising a protein binding reagent conjugated with an oligonucleotide.Type: ApplicationFiled: August 22, 2022Publication date: April 27, 2023Inventors: Christina Fan, Christina Chang, Eleen Shum, Olaf Zoellner, Nidhanjali Bansal, James Ghadiali, Gretchen Yinbon Lam, Eric Jensen, Jurg Rohrer, Brent Gaylord, Jody Martin, Jason G. Vidal
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Patent number: 11460468Abstract: Some embodiments disclosed herein provide a plurality of compositions each comprising a protein binding reagent conjugated with an oligonucleotide. The oligonucleotide comprises a unique identifier for the protein binding reagent it is conjugated with, and the protein binding reagent is capable of specifically binding to a protein target. Further disclosed are methods and kits for quantitative analysis of a plurality of protein targets in a sample and for simultaneous quantitative analysis of protein and nucleic acid targets in a sample. Also disclosed herein are systems and methods for preparing a labeled biomolecule reagent, including a labeled biomolecule agent comprising a protein binding reagent conjugated with an oligonucleotide.Type: GrantFiled: September 25, 2017Date of Patent: October 4, 2022Assignee: Becton, Dickinson and CompanyInventors: Christina Fan, Christina Chang, Eleen Shum, Olaf Zoellner, Nidhanjali Bansal, James Ghadiali, Gretchen Yinbon Lam, Eric Jensen, Jurg Rohrer, Brent Gaylord, Jody Martin, Jason G. Vidal
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Patent number: 11315661Abstract: Aspects of the present disclosure include systems for use in preparing an epitope tagged biomolecule reagent. A reagent preparatory apparatus for preparing the epitope tagged biomolecule reagent from an activated biomolecule and activated epitope tag is also described. Methods for communicating and receiving an epitope tagged biomolecule reagent request and preparing the subject epitope tagged biomolecule reagents are also provided.Type: GrantFiled: February 1, 2018Date of Patent: April 26, 2022Assignee: BECTON, DICKINSON AND COMPANYInventors: Jody Martin, Adam Wright, Paul Waterman, James Ghadiali
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Publication number: 20210263019Abstract: Disclosed herein include methods, compositions, and kits suitable for spectral unmixing and compensation in multi-parameter flow cytometry. Disclosed herein include reagents which comprise two antibodies conjugated to one another to form a bispecific reagent (e.g., a bispecific probe). A first antibody can have affinity for a highly expressed antigen on the surface of a cell of interest and a second antibody can have affinity for each of the antibody-dye conjugates in a multi-parameter panel. There are provided, in some embodiments, methods of using the bispecific reagent to determine spillover, perform compensation, and generate a compensation matrix.Type: ApplicationFiled: February 24, 2021Publication date: August 26, 2021Inventors: Jody Martin, James Ghadiali, Mirko Corselli, Adam Wright, Aaron Jacob Tyznik
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Patent number: 11099190Abstract: Fluorogenic sensors for a target analyte are provided. The fluorogenic sensors can include a water soluble light harvesting multichromophore and a fluorogenic dye covalently linked to the multichromophore and in energy-receiving proximity therewith. Aspects of the fluorogenic sensors include selective recognition of the target analyte by chemical reaction or by specific binding. In some cases, the linked fluorogenic dye includes a lactone or spiro-lactone group that is configured to chemically react with a target analyte via lactone ring-opening to produce a fluorescent dye configured for excitation by the multichromophore. Also provided are labelled specific binding members that include the subject dyes. Methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule in which the subject fluorogenic sensors find use are provided. Systems, devices and kits for practicing the subject methods are provided.Type: GrantFiled: December 6, 2016Date of Patent: August 24, 2021Assignee: BECTON, DICKINSON AND COMPANYInventors: Jody Martin, Robert J. Radford, James Ghadiali
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Publication number: 20210222244Abstract: Systems, methods, compositions, and kits for measuring secreted factors from cells are disclosed herein, including those capable of determining single cell secretion activity and protein expression and/or gene expression simultaneously. Disclosed herein include bispecific probes comprising an anchor probe capable of specifically binding to a surface cellular target of a cell, and a capture probe capable of specifically binding to a secreted factor secreted by a cell that is associated with the capture probe. Also disclosed herein include secreted factor-binding reagents capable of specifically binding to a secreted factor bound by a capture probe, where a secreted factor-binding reagent can comprise a secreted factor-binding reagent specific oligonucleotide comprising a unique factor identifier sequence for the secreted factor-binding reagent.Type: ApplicationFiled: January 15, 2021Publication date: July 22, 2021Inventors: Jody Martin, Mirko Corselli, James Ghadiali, Feng Ge, Chad Sisouvanthong
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Publication number: 20200385780Abstract: Disclosed herein include systems, methods, compositions, and kits for sample identification. A sample indexing composition can comprise, for example, a protein binding reagent associated with a sample indexing oligonucleotide. Different sample indexing compositions can include sample indexing oligonucleotides with different sequences. Sample origin of cells can be identified based on the sequences of the sample indexing oligonucleotides. Sample indexing oligonucleotides can be barcoded using barcoded and lengthened using daisy-chaining primers.Type: ApplicationFiled: April 14, 2020Publication date: December 10, 2020Inventors: Christina Chang, Christina Fan, Eleen Shum, Jody Martin, Nidhanjali Bansal, James Ghadiali, Katherine Lazaruk, Gretchen Yinbon Lam
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Publication number: 20200232032Abstract: Disclosed herein include systems, methods, compositions, and kits for determining protein expression and gene expression simultaneously and for sample indexing. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) comprises one or more of a unique molecular label sequence, a primer adapter, antibody-specific barcode sequence, an alignment sequence, and/or a poly(A) sequence. In some embodiments, the oligonucleotide is associated with the cellular component-binding reagent via a linker (e.g., 5AmMC12).Type: ApplicationFiled: January 21, 2020Publication date: July 23, 2020Inventors: Eleen Shum, Katherine Lazaruk, Dennis E. Prosen, Devon Jensen, David Rosenfeld, Janice H. Lai, James Ghadiali, Christina Chang
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Patent number: 10676779Abstract: Disclosed herein include systems, methods, compositions, and kits for sample identification. A sample indexing composition can comprise, for example, a protein binding reagent associated with a sample indexing oligonucleotide. Different sample indexing compositions can include sample indexing oligonucleotides with different sequences. Sample origin of cells can be identified based on the sequences of the sample indexing oligonucleotides. Sample indexing oligonucleotides can be barcoded using barcoded and lengthened using daisy-chaining primers.Type: GrantFiled: March 27, 2018Date of Patent: June 9, 2020Assignee: Becton, Dickinson and CompanyInventors: Christina Chang, Christina Fan, Eleen Shum, Jody Martin, Nidhanjali Bansal, James Ghadiali, Katherine Lazaruk, Gretchen Yinbon Lam
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Patent number: 10472521Abstract: Photostable polymeric tandem dyes are provided. The photostable polymeric tandem dyes include a water soluble light harvesting multichromophore and a luminescent metal complex covalently linked to the multichromophore and in energy-receiving proximity therewith. Also provided are labelled specific binding members that include the subject dyes. Methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule in which the subject polymeric tandem dyes find use are also provided. Systems and kits for practicing the subject methods are also provided.Type: GrantFiled: December 6, 2016Date of Patent: November 12, 2019Assignee: BECTON, DICKINSON AND COMPANYInventors: Robert J. Radford, Douglas B. Sherman, Jody Martin, James Ghadiali
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Patent number: 10408759Abstract: Methods for producing a high resolution image of a sample are provided. In some embodiments, the method includes: detecting first and second sets of spatially-dependent emission signals from a sample labeled with a fluorescent polymeric dye; and producing a high resolution fluorescence image of the sample from the detected first and second sets of spatially-dependent emission signals. In some embodiments, the sample is a cell. Also provided are systems for imaging a sample that include a high resolution light microscope including a light source configured to irradiate a field of view with an excitation light; a photodetector configured to detect an emission signal: and a polarization modulator disposed in the light pathway between the light source and the photodetector; and a sample labelled with a polymeric dye and disposed in the field of view.Type: GrantFiled: April 25, 2016Date of Patent: September 10, 2019Assignee: BECTON, DICKINSON AND COMPANYInventor: James Ghadiali
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Publication number: 20180364245Abstract: Fluorogenic sensors for a target analyte are provided. The fluorogenic sensors can include a water soluble light harvesting multichromophore and a fluorogenic dye covalently linked to the multichromophore and in energy-receiving proximity therewith. Aspects of the fluorogenic sensors include selective recognition of the target analyte by chemical reaction or by specific binding. In some cases, the linked fluorogenic dye includes a lactone or spiro-lactone group that is configured to chemically react with a target analyte via lactone ring-opening to produce a fluorescent dye configured for excitation by the multichromophore. Also provided are labelled specific binding members that include the subject dyes. Methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule in which the subject fluorogenic sensors find use are provided. Systems, devices and kits for practicing the subject methods are provided.Type: ApplicationFiled: December 6, 2016Publication date: December 20, 2018Inventors: Jody MARTIN, Robert J. Radford, James Ghadiali
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Publication number: 20180346970Abstract: Disclosed herein include systems, methods, compositions, and kits for sample identification. A sample indexing composition can comprise, for example, a protein binding reagent associated with a sample indexing oligonucleotide. Different sample indexing compositions can include sample indexing oligonucleotides with different sequences. Sample origin of cells can be identified based on the sequences of the sample indexing oligonucleotides. Sample indexing oligonucleotides can be barcoded using barcoded and lengthened using daisy-chaining primers.Type: ApplicationFiled: March 27, 2018Publication date: December 6, 2018Inventors: Christina Chang, Christina Fan, Eleen Shum, Jody Martin, Nidhanjali Bansal, James Ghadiali, Katherine Lazaruk, Gretchen Yinbon Lam
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Publication number: 20180232484Abstract: Aspects of the present disclosure include systems for use in preparing an epitope tagged biomolecule reagent. A reagent preparatory apparatus for preparing the epitope tagged biomolecule reagent from an activated biomolecule and activated epitope tag is also described. Methods for communicating and receiving an epitope tagged biomolecule reagent request and preparing the subject epitope tagged biomolecule reagents are also provided.Type: ApplicationFiled: February 1, 2018Publication date: August 16, 2018Inventors: Jody Martin, Adam Wright, Paul Waterman, James Ghadiali
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Publication number: 20180088112Abstract: Some embodiments disclosed herein provide a plurality of compositions each comprising a protein binding reagent conjugated with an oligonucleotide. The oligonucleotide comprises a unique identifier for the protein binding reagent it is conjugated with, and the protein binding reagent is capable of specifically binding to a protein target. Further disclosed are methods and kits for quantitative analysis of a plurality of protein targets in a sample and for simultaneous quantitative analysis of protein and nucleic acid targets in a sample. Also disclosed herein are systems and methods for preparing a labeled biomolecule reagent, including a labeled biomolecule agent comprising a protein binding reagent conjugated with an oligonucleotide.Type: ApplicationFiled: September 25, 2017Publication date: March 29, 2018Inventors: Christina Fan, Christina Chang, Eleen Shum, Olaf Zoellner, Nidhanjali Bansal, James Ghadiali, Gretchen Yinbon Lam, Eric Jensen, Jurg Rohrer, Brent Gaylord, Jody Martin, Jason G. Vidal
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Publication number: 20170370847Abstract: Methods for producing a high resolution image of a sample are provided. In some embodiments, the method includes: detecting first and second sets of spatially-dependent emission signals from a sample labeled with a fluorescent polymeric dye; and producing a high resolution fluorescence image of the sample from the detected first and second sets of spatially-dependent emission signals. In some embodiments, the sample is a cell. Also provided are systems for imaging a sample that include a high resolution light microscope including a light source configured to irradiate a field of view with an excitation light; a photodetector configured to detect an emission signal: and a polarization modulator disposed in the light pathway between the light source and the photodetector; and a sample labelled with a polymeric dye and disposed in the field of view.Type: ApplicationFiled: April 25, 2016Publication date: December 28, 2017Inventor: James Ghadiali
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Publication number: 20170248587Abstract: Proteinaceous specific binding members that specifically bind to a polymeric dye are provided. Also provided are methods of using the specific binding members, e.g., in separating a polymeric dye-labeled cell from a sample, in analyte detection, etc., as described herein. Kits and systems for practicing the subject methods are also provided.Type: ApplicationFiled: November 11, 2015Publication date: August 31, 2017Applicant: Becton, Dickinson and CompanyInventors: James Ghadiali, Jody Martin
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Publication number: 20170174892Abstract: Photostable polymeric tandem dyes are provided. The photostable polymeric tandem dyes include a water soluble light harvesting multichromophore and a luminescent metal complex covalently linked to the multichromophore and in energy-receiving proximity therewith. Also provided are labelled specific binding members that include the subject dyes. Methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule in which the subject polymeric tandem dyes find use are also provided. Systems and kits for practicing the subject methods are also provided.Type: ApplicationFiled: December 6, 2016Publication date: June 22, 2017Inventors: Robert J. Radford, Douglas B. Sherman, Jody Martin, James Ghadiali