Abstract: To achieve full development of the ripe phenotype, climacteric fruits, such as tomato, apple and banana, require synthesis, perception and signal transduction of the plant hormone ethylene. The non-ripening phenotype of the dominant Green-ripe (Gr) and Never-ripe 2 (Nr-2) mutants of tomato is the result of reduced ethylene responsiveness in fruit tissues. In addition a subset of ethylene responses associated with floral senescence, abscission and root elongation are also impacted in mutant plants but to a lesser extent. Using positional cloning we have identified an identical 334 by deletion in a gene of unknown biochemical function residing at the Gr/Nr-2 locus. Consistent with a dominant gain of function mutation, this deletion causes ectopic expression of GR/NR-2, which in turn leads to ripening inhibition. A CaMV35:GR transgene recreates the Gr/Nr-2 mutant phenotype but does not lead to a global reduction in ethylene responsiveness suggesting tissue-specific modulation of ethylene responses in tomato.

Abstract: The wire spool guide assembly is a conduit through which wire is dispensed in a controlled and orderly manner. The assembly has at least one wire spool guide, a handle bar and a spindle. The spindle may be a single spindle or part of a wire spool cart. The spindle is used to hold wire spools and the handle bar. The handle bar serves to hold the guide. The guide includes an eyebolt rotatably mounted to a support frame via a ball bearing. The eyebolt guides wire as it is dispensed from the spool acquiescing to the direction of the user's pull. Friction, created as wire is pulled through the eyebolt, slows the speed at which the spool rotates on the spindle. The frame of the guide, however, is fixed on the handle bar by a fastener disposed at a bottom end of the guide.

Abstract: The wire spool guide assembly is a conduit through which wire is dispensed in a controlled and orderly manner. The assembly has at least one wire spool guide, a handle bar and a spindle. The spindle may be a single spindle or part of a wire spool cart. The spindle is used to hold wire spools and the handle bar. The handle bar serves to hold the guide. The guide includes an eyebolt rotatably mounted to a support frame via a ball bearing. The eyebolt guides wire as it is dispensed from the spool acquiescing to the direction of the user's pull. Friction, created as wire is pulled through the eyebolt, slows the speed at which the spool rotates on the spindle. The frame of the guide, however, is fixed on the handle bar by a fastener disposed at a bottom end of the guide.

Abstract: The current invention provides nucleic acid sequences encoding the NOR gene. Compositions comprising this sequence are described, as are plants transformed with such compositions. Further provided are methods for the expression of the NOR gene. The methods of the invention include the direct creation of transgenic plants with the NOR gene by genetic transformation, as well as by plant breeding methods. The sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.

Abstract: The current invention provides nucleic acid sequences encoding the RIN and MC genes. Compositions comprising these sequences are described, as are plants transformed with such compositions. Further provided are methods for the expression of the RIN and MC genes. The methods of the invention include the direct creation of transgenic plants with the RIN and MC genes by genetic transformation, as well as by plant breeding methods. The sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.

Abstract: The current invention provides nucleic acid sequences encoding the RIN and MC genes. Compositions comprising these sequences are described, as are plants transformed with such compositions. Further provided are methods for the expression of the RIN and MC genes. The methods of the invention include the direct creation of transgenic plants with the RIN and MC genes by genetic transformnation, as well as by plant breeding methods. The sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.

Abstract: The current invention provides nucleic acid sequences encoding the NOR gene. Compositions comprising this sequence are described, as are plants transformed with such compositions. Further provided are methods for the expression of the NOR gene. The methods of the invention include the direct creation of transgenic plants with the NOR gene by genetic transformation, as well as by plant breeding methods. The sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.

Abstract: Edible fruit, seed and vegetables of transgenic plants modified to produce a sweetening protein such as monellin or thaumatin are useful in preparing food compositions which have enhanced sweetness improved flavor. Expression systems for the genes encoding sweetening proteins compatible with plant systems and designed to enhance the production of these proteins in the edible portions of plants, and methods for producing sweetened fruit, seeds and vegetables are described.

Abstract: The present invention provides a method for reducing fruit softening and cell wall polysaccharide degradation by inhibiting endo-1,4-.beta.-glucanase activity using antisense nucleic acid constructions.

Abstract: The present invention provides a method for reducing fruit softening and cell wall polysaccharide degradation by inhibiting endo-1,4-.beta.-glucanase activity using antisense nucleic acid constructions.

Abstract: The present invention provides a method for reducing fruit softening and cell wall polysaccharide degradation by inhibiting endo-1,4-.beta.-glucanase activity using antisense DNA constructions.

Abstract: Edible fruit, seed and vegetables of transgenic plants modified to produce a sweetening protein such as monellin or thaumatin are useful in preparing food compositions which have enhanced sweetness improved flavor. Expression systems for the genes encoding sweetening proteins compatible with plant systems and designed to enhance the production of these proteins in the edible portions of plants, and methods for producing sweetened fruit, seeds and vegetables are described.

Abstract: The present invention provides a method for reducing fruit softening and cell wall polysaccharide degradation by inhibiting endo-1,4-.beta.-glucanase activity using antisense DNA constructions.