Patents by Inventor James H. Jett
James H. Jett has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8932520Abstract: Method and system for uniformly spacing particles in a flowing system comprising suspending particles in an elongated fluid filled cavity; exposing said cavity to an axial acoustic standing wave field, wherein said axial acoustic standing wave field drives said particles to nodal and anti-nodal positions along the center axis of said cavity to result in uniformly spaced particles; and focusing said particles to the center axis of said cavity.Type: GrantFiled: August 9, 2012Date of Patent: January 13, 2015Assignee: Los Alamos National Security, LLCInventors: Gregory R Goddard, Gregory Kaduchak, James H Jett, Steven W Graves
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Publication number: 20040265835Abstract: A method for identifying and sorting variants of a chosen enzyme is disclosed. Enzyme variants of a chosen enzyme are obtained and then linked to their corresponding genetic code through any of a family of suitable surface display methods. The enzyme variants, now displayed on the surface of a biological particle such as a phage, virus, yeast, or bacterium are then encapsulated in a vesicle containing an enzyme activity-sensitive assay reagent. The enzyme variant is thus exposed to the assay reagent, and displays a signal using the enzyme activity-sensitive assay reagent in a manner proportionate to the levels of activity of the enzyme, thus rendering the vesicles suitable for mechanical sorting based on these levels. The vesicles are then sorted using methods known in the art to isolate those variants exhibiting possibly beneficial variations in enzyme function.Type: ApplicationFiled: June 30, 2003Publication date: December 30, 2004Inventors: David M. Lemaster, James H. Jett
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Patent number: 6309886Abstract: Apparatus and method enable imaging multiple fluorescent sample particles in a single flow channel. A flow channel defines a flow direction for samples in a flow stream and has a viewing plane perpendicular to the flow direction. A laser beam is formed as a ribbon having a width effective to cover the viewing plane. Imaging optics are arranged to view the viewing plane to form an image of the fluorescent sample particles in the flow stream, and a camera records the image formed by the imaging optics.Type: GrantFiled: November 24, 1999Date of Patent: October 30, 2001Assignee: The Regents of the University of CaliforniaInventors: W. Patrick Ambrose, W. Kevin Grace, Peter M. Goodwin, James H. Jett, Alan Van Orden, Richard A. Keller
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Patent number: 6049380Abstract: Single fluorescent molecules in a flowing sample stream are distinguished and identified using only a single laser excitation wavelength. A sample stream is formed containing a dilute mixture of single molecule fluorophores, wherein each one of the fluorophores is serially ordered in the sample stream. The sample stream is illuminated with s single excitation wavelength laser effective to excite each fluorophore one at a time. Fluorescence emission photons from each said fluorophore are detected. A burst size is determined for each fluorophore to identify each fluorophore. A pulsed laser may be used, where burst size and an intra-burst fluorescence decay rate for each fluorophore are determined simultaneously from the detected fluorescence emission photons. The burst size and the decay rate are correlated to identify each fluorophore.Type: GrantFiled: October 9, 1998Date of Patent: April 11, 2000Assignee: Regents of the University of CaliforniaInventors: Peter M. Goodwin, James H. Jett, Richard A. Keller, Alan K. Van Orden, Nicholas P. Machara
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Patent number: 5879625Abstract: Optical selection and collection of DNA fragments. The present invention includes the optical selection and collection of large (>.mu.g) quantities of clonable, chromosome-specific DNA from a sample of chromosomes. Chromosome selection is based on selective, irreversible photoinactivation of unwanted chromosomal DNA. Although more general procedures may be envisioned, the invention is demonstrated by processing chromosomes in a conventional flow cytometry apparatus, but where no droplets are generated. All chromosomes in the sample are first stained with at least one fluorescent analytic dye and bonded to a photochemically active species which can render chromosomal DNA unclonable if activated. After passing through analyzing light beam(s), unwanted chromosomes are irradiated using light which is absorbed by the photochemically active species, thereby causing photoinactivation.Type: GrantFiled: October 17, 1997Date of Patent: March 9, 1999Assignee: The Regents of the University of CaliforniaInventors: Mary C. Roslaniec, John C. Martin, James H. Jett, L. Scott Cram
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Patent number: 5707808Abstract: Optical selection and collection of DNA fragments. The present invention includes the optical selection and collection of large (>.mu.g) quantities of clonable, chromosome-specific DNA from a sample of chromosomes. Chromosome selection is based on selective, irreversible photoinactivation of unwanted chromosomal DNA. Although more general procedures may be envisioned, the invention is demonstrated by processing chromosomes in a conventional flow cytometry apparatus, but where no droplets are generated. All chromosomes in the sample are first stained with at least one fluorescent analytic dye and bonded to a photochemically active species which can render chromosomal DNA unclonable if activated. After passing through analyzing light beam(s), unwanted chromosomes are irradiated using light which is absorbed by the photochemically active species, thereby causing photoinactivation.Type: GrantFiled: April 15, 1996Date of Patent: January 13, 1998Assignee: The Regents of the University of CaliforniaInventors: Mary C. Roslaniec, John C. Martin, James H. Jett, L. Scott Cram
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Patent number: 5558998Abstract: A method is provided for sizing DNA fragments using high speed detection systems, such as flow cytometry to determine unique characteristics of DNA pieces from a sample. In one characterization the DNA piece is fragmented at preselected sites to produce a plurality of DNA fragments. The DNA piece or the resulting DNA fragments are treated with a dye effective to stain stoichiometrically the DNA piece or the DNA fragments. The fluorescence from the dye in the stained fragments is then examined to generate an output functionally related to the number of nucleotides in each one of the DNA fragments. In one embodiment, the intensity of the fluorescence emissions from each fragment is linearly related to the fragment length. The distribution of DNA fragment sizes forms a characterization of the DNA piece for use in forensic and research applications.Type: GrantFiled: June 5, 1995Date of Patent: September 24, 1996Assignee: The Regents of the Univ. of CaliforniaInventors: Mark L. Hammond, James H. Jett, Richard A. Keller, Babetta L. Marrone, John C. Martin
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Patent number: 5405747Abstract: Method for rapid-base sequencing in DNA and RNA with two-base labeling and employing fluorescent detection of single molecules at two wavelengths. Bases modified to accept fluorescent labels are used to replicate a single DNA or RNA strand to be sequenced. The bases are then sequentially cleaved from the replicated strand, excited with a chosen spectrum of electromagnetic radiation, and the fluorescence from individual, tagged bases detected in the order of cleavage from the strand.Type: GrantFiled: March 7, 1994Date of Patent: April 11, 1995Assignee: The Regents of the University of California Office of Technology TransferInventors: James H. Jett, Richard A. Keller, John C. Martin, Richard G. Posner, Babetta L. Marrone, Mark L. Hammond, Daniel J. Simpson
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Patent number: 5085673Abstract: Method and apparatus for removing material from a gas. A mist created by a piezoelectric ultrasonic transducer is contacted with the gas and both gas and mist are passed through baffled separators. Liquid effluent from the separators contains solid material removed from the gas and gaseous material which reacted with the liquid or was absorbed by the liquid. The invention is useful for collecting a sample of material in a gas, such as a vapor in the atmosphere, and in cleaning a gas. A relatively concentrated solution of a material present in a gas in a very small concentration can be obtained.Type: GrantFiled: March 19, 1991Date of Patent: February 4, 1992Assignee: The United States of America as represented by the United States Department of EnergyInventors: Bill F. Bentley, James H. Jett, John C. Martin, George C. Saunders
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Patent number: 4962037Abstract: A method is provided for the rapid base sequencing of DNA or RNA fragments wherein a single fragment of DNA or RNA is provided with identifiable bases and suspended in a moving flow stream. An exonuclease sequentially cleaves individual bases from the end of the suspended fragment. The moving flow stream maintains the cleaved bases in an orderly train for subsequent detection and identification. In a particular embodiment, individual bases forming the DNA or RNA fragments are individually tagged with a characteristic fluorescent dye. The train of bases is then excited to fluorescence with an output spectrum characteristic of the individual bases. Accordingly, the base sequence of the original DNA or RNA fragment can be reconstructed.Type: GrantFiled: October 7, 1987Date of Patent: October 9, 1990Assignee: United States of AmericaInventors: James H. Jett, Richard A. Keller, John C. Martin, Robert K. Moyzis, Robert L. Ratliff, E. Brooks Shera, Carleton C. Stewart
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Patent number: 4573796Abstract: The disclosure is directed to an apparatus for eliminating background interference during fluorescence measurements in a multiple laser flow cytometer. A biological particle stained with fluorescent dyes is excited by a laser. A fluorescence detector detects the fluorescence. The particle scatters light and a gate signal is generated and delayed until the biological particle reaches the next laser. The delayed signal turns on this next laser, which excites a different stained component of the same biological particle.Type: GrantFiled: January 6, 1984Date of Patent: March 4, 1986Assignee: The United States of America as represented by the United States Department of EnergyInventors: John C. Martin, James H. Jett