Abstract: Methods, devices, systems, and kits for electro-mechanical cell transfection are provided. A device includes a first electrode, a second electrode, and an active zone therebetween where an electrical potential difference applied to the first and second electrodes generates an electric field in the active zone sufficient to transfect at least a subset of the cells flowing in the active zone.

Abstract: Devices, systems, and kits for cell transfection are provided. A device includes a first electrode, a second electrode, and an electroporation zone therebetween where an electrical potential difference applied to the first and second electrodes generates an electric field in the electroporation zone sufficient to transfect at least a subset of the cells in the flow path. Methods of introducing a composition into at least a portion of a plurality of cells using the devices, systems, and kits of the invention are also provided.

Abstract: A composition comprising (A) from 30 wt % to 70 wt % of a propylene component including at least one propylene based polymer having a propylene content of at least 70.0 wt %, based on the total weight of the propylene based polymer, and a melt flow rate from 1.0 g/10 min to 100.0 g/10 min (ASTM D-1238 at 230° C., 2.16 kg); (B) from 1 wt % to 20 wt % of an ethylene component including at least one ethylene based polymer having an ethylene content of at least 85.0 wt %, based on the total weight of the ethylene based polymer, and a melt index from 0.1 g/10 min to 50.0 g/10 min (ASTM D-1238 at 190° C., 2.16 kg); (C) from 1 wt % to 40 wt % of an olefin block copolymer; and at least one of (D) from 1 wt % to 40 wt % of a polyolefin elastomer and (E) from 1 wt % to 30 wt % of a filler.

Abstract: A submersible habitat chamber providing a contained environment for repairing subsea cable is provided. The habitat chamber includes first and second shell parts assembled to at least partly define the contained environment, a first seal provided between the first and second shell parts, a cable seal provided for engagement with the cable, and at least one access port through at least one of the shell parts to allow a user to access the contained environment from outside the habitat. A method of using the habitat chamber and a kit for assembly of the chamber are also provided.

Abstract: Disclosed herein are antibody-coupled T cell receptor (ACTR) polypeptides comprising: a CD16A extracellular domain, a transmembrane domain, one or more co-stimulatory signaling domains, at least one of which is a CD28 co-stimulatory signaling domain, and a CD3z cytoplasmic signaling domain. Also disclosed herein are genetically engineered immune cells, expressing: a first polypeptide which is an antibody-coupled T cell receptor (ACTR); and a second polypeptide that elicits a co-stimulatory signal as well as methods of enhancing antibody-dependent cell cytotoxicity (ADCC) in a subject comprising administering to a subject in need thereof a therapeutically effective amount of a therapeutic antibody and an effective amount of immune cells (e.g., T lymphocytes and/or NK cells) expressing an antibody-coupled T-cell receptor (ACTR) polypeptide.

Abstract: Devices, systems, and kits for cell electroporation are provided. A device includes a first electrode, a second electrode, and an electroporation zone therebetween where an electrical potential difference applied to the first and second electrodes generates an electric field in the electroporation zone sufficient to electroporate at least a subset of the cells in the flow path. Methods of introducing a composition into at least a portion of a plurality of cells using the devices, systems, and kits of the invention are also provided.

Abstract: A composition comprising (A) from 30 wt % to 70 wt % of a propylene component including at least one propylene based polymer having a propylene content of at least 70.0 wt %, based on the total weight of the propylene based polymer, and a melt flow rate from 1.0 g/10 min to 100.0 g/10 min (ASTM D-1238 at 230° C., 2.16 kg); (B) from 1 wt % to 20 wt % of an ethylene component including at least one ethylene based polymer having an ethylene content of at least 85.0 wt %, based on the total weight of the ethylene based polymer, and a melt index from 0.1 g/10 min to 50.0 g/10 min (ASTM D-1238 at 190° C., 2.16 kg); (C) from 1 wt % to 40 wt % of an olefin block copolymer; and at least one of (D) from 1 wt % to 40 wt % of a polyolefin elastomer and (E) from 1 wt % to 30 wt % of a filler.

Abstract: A submersible habitat chamber providing a contained environment for repairing subsea cable is provided. The habitat chamber includes first and second shell parts assembled to at least partly define the contained environment, a first seal provided between the first and second shell parts, a cable seal provided for engagement with the cable, and at least one access port through at least one of the shell parts to allow a user to access the contained environment from outside the habitat. A method of using the habitat chamber and a kit for assembly of the chamber are also provided.