Abstract: In an aspect, disclosed herein are physiological devices and systems, and components thereof, used to evaluate cardiac parameters and arrhythmogenic mechanisms. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.

Abstract: In some examples, an apparatus can include a syringe body including an electrical interface at a side surface of the syringe body, an interface at an end of the syringe body including an output at a distal surface of the syringe body, a print material particles reservoir located in the syringe body, and a structure to adapt a volume of the print material particles reservoir to move print material particles out of the print material particles reservoir through the output, where in response to the volume adapting structure moving from a first position to a second position, a signal is transmitted by the electrical interface.

Abstract: Examples described herein relate to a supply valve consistent with the disclosure. For instance, a supply valve to supply print particles to a receptacle, the supply valve may comprise a print particle exit, a print particle entrance perpendicular to the print particle exit, and a liner adjacent to the print particle exit, where the liner is to wipe print particles, when present, from the supply valve responsive to the transition of the supply valve between a closed position and an open position.

Abstract: Disclosed herein are microfluidic systems with recirculation of fluid and computer-implemented methods of calculating conditions within the microfluidic systems. The microfluidic systems include a computing device and a microfluidic device having first and second reservoirs, at least one chamber, and a fluid path connecting the first reservoir, the chamber, and the second reservoir. The methods for calculating conditions include receiving a first reservoir fluid volume, a second reservoir fluid volume, a first concentration, and a second concentration. The methods further include receiving a time-dependent imposed pressure difference between the first reservoir and the second reservoir, then determining a hydraulic pressure difference and an effective pressure difference. The effective pressure difference is used to account for reactions occurring within the microfluidic device and to determine the value of the condition within the microfluidic device.

Abstract: In an aspect, disclosed herein are physiological devices and systems, and components thereof, used to evaluate cardiac parameters and arrhythmogenic mechanisms. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.

Abstract: Disclosed herein are microfluidic systems with recirculation of fluid and computer-implemented methods of calculating conditions within the microfluidic systems. The microfluidic systems include a computing device and a microfluidic device having first and second reservoirs, at least one chamber, and a fluid path connecting the first reservoir, the chamber, and the second reservoir. The methods for calculating conditions include receiving a first reservoir fluid volume, a second reservoir fluid volume, a first concentration, and a second concentration. The methods further include receiving a time-dependent imposed pressure difference between the first reservoir and the second reservoir, then determining a hydraulic pressure difference and an effective pressure difference. The effective pressure difference is used to account for reactions occurring within the microfluidic device and to determine the value of the condition within the microfluidic device.

Abstract: A pumpless microfluidic system is disclosed that can be used to mimic the interaction of organ systems with the immune system. Also disclosed is a method for mimicking an immune system, comprising culturing a plurality of organ cells and at least one population of immune cells in the disclosed pumpless microfluidic system under physiological conditions. The method can further comprise activating an immune reaction in the pumpless microfluidic system, continuing the culture for a defined period, collecting a sample of culture medium from the system, and assaying the sample for one or more indicators of an immune response.

Abstract: Disclosed herein are microfluidic systems with recirculation of fluid and computer-implemented methods of calculating conditions within the microfluidic systems. The microfluidic systems include a computing device and a microfluidic device having first and second reservoirs, at least one chamber, and a fluid path connecting the first reservoir, the chamber, and the second reservoir. The methods for calculating conditions include receiving a first reservoir fluid volume, a second reservoir fluid volume, a first concentration, and a second concentration. The methods further include receiving a time-dependent imposed pressure difference between the first reservoir and the second reservoir, then determining a hydraulic pressure difference and an effective pressure difference. The effective pressure difference is used to account for reactions occurring within the microfluidic device and to determine the value of the condition within the microfluidic device.

Abstract: Disclosed herein are cell culture analog devices, systems and methods for applying stimuli to components containing different cell types and recording the cell responses before, during, and after a stimulus (for example, a drug, metabolite, toxin, or electrical stimulus) is introduced. Responses can be stored to a database and compared to previous results. By analyzing how each cell type responds to various stimulation parameters, for example, by using multivariate analyses, cell signaling pathway information can be determined or new pathways can be discovered. In some implementations, an individual component interfaces with a specific cell type. This facilitates readout of the cell response to the stimulation. Various components can also interface with each other, such that the behavior of one cell type can affect a cell type in another component. Once assembled, the system is plugged into readout electronics and a programmable electrical stimulator.

Abstract: Disclosed herein are devices comprising one or more cantilevers comprising one or more neuromuscular junctions formed by a co-culture of myotubes and motoneurons. Disclosed herein are methods of using the disclosed devices comprising one or more cantilevers. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.

Abstract: In an aspect, disclosed herein are physiological devices and systems, and components thereof, used to evaluate cardiac parameters and arrhythmogenic mechanisms. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.

Abstract: A non-contact voltage detector for detecting and indicating voltage. The non-contact voltage detector includes a housing having a voltage detection probe and plurality of light sources. The housing further includes an integral power source that is rechargeable via an external power source coupled to a user input port. The power source provides electrical energy to the non-contact voltage detector that further includes a controller and control circuit that is operably connected to at least one light source. The controller and control circuit can detect electrical energy at the user input port, detect the charge state of the integral power source, charge the integral power source, and emit one or more charge state light indicators when the state of the integral power source changes. In addition, the light sources can indicate the presence of voltage at the probe and can be independently activated by one or more switches.

Abstract: A pumpless microfluidic system is disclosed that can be used to mimic the interaction of organ systems with the immune system. Also disclosed is a method for mimicking an immune system, comprising culturing a plurality of organ cells and at least one population of immune cells in the disclosed pumpless microfluidic system under physiological conditions. The method can further comprise activating an immune reaction in the pumpless microfluidic system, continuing the culture for a defined period, collecting a sample of culture medium from the system, and assaying the sample for one or more indicators of an immune response.

Abstract: Disclosed herein are microfluidic systems with recirculation of fluid and computer-implemented methods of calculating conditions within the microfluidic systems. The microfluidic systems include a computing device and a microfluidic device having first and second reservoirs, at least one chamber, and a fluid path connecting the first reservoir, the chamber, and the second reservoir. The methods for calculating conditions include receiving a first reservoir fluid volume, a second reservoir fluid volume, a first concentration, and a second concentration. The methods further include receiving a time-dependent imposed pressure difference between the first reservoir and the second reservoir, then determining a hydraulic pressure difference and an effective pressure difference. The effective pressure difference is used to account for reactions occurring within the microfluidic device and to determine the value of the condition within the microfluidic device.

Abstract: Disclosed herein are cell culture analog devices, systems and methods for applying stimuli to components containing different cell types and recording the cell responses before, during, and after a stimulus (for example, a drug, metabolite, toxin, or electrical stimulus) is introduced. Responses can be stored to a database and compared to previous results. By analyzing how each cell type responds to various stimulation parameters, for example, by using multivariate analyses, cell signaling pathway information can be determined or new pathways can be discovered. In some implementations, an individual component interfaces with a specific cell type. This facilitates readout of the cell response to the stimulation. Various components can also interface with each other, such that the behavior of one cell type can affect a cell type in another component. Once assembled, the system is plugged into readout electronics and a programmable electrical stimulator.

Abstract: The invention discloses a bio-MEMS transducer comprising a cultured myotube and a piezoelectric microcantilever having the myotube attached thereto along a lengthwise extent of said microcantilever. The transducer may include an input/output processor operably connected with said piezoelectric microcantilever to process electrical signals received therefrom and to send electrical signals thereto. The invention may operate as a biosensor wherein the attached myotube contracts on contact with a sensed agent, the myotube contraction deflecting the microcantilever to generate a piezoelectric signal therefrom. The invention may also be used as a biosensor for quantitating physiologic response to an agent by measuring deflection of the cantilever caused by myotube contraction elicited by contact with the agent; and correlating the measurement to effectiveness of the sensed agent in causing a myotube physiologic response.

Abstract: The invention provides a method of co-culturing mammalian muscle cells and mammalian motoneurons. The method comprises preparing one or more carriers coated with a covalently bonded monolayer of trimethoxysilylpropyl diethylenetriamine (DETA); suspending isolated fetal mammalian skeletal muscle cells in serum-free medium according to medium composition 1; suspending isolated fetal mammalian spinal motoneurons in serum-free medium according to medium composition 1; plating the suspended muscle cells onto the one or more carriers at a predetermined density and allowing the muscle cells to attach; plating the suspended motoneurons at a predetermined density onto the one or more carriers and allowing the motoneurons to attach; covering the one or more carriers with a mixture of medium composition 1 and medium composition 2; and incubating the carriers covered in the media mixture.

Abstract: A method for forming neuromuscular junctions includes forming functional neuromuscular junctions between motoneurons and muscle cells by co-culturing one or more human motoneurons and one or more rat muscle cells in a substantially serum-free medium. A synthetic mammalian neuromuscular junction includes a human motoneuron functionally linked to a rat muscle cell in a substantially serum-free medium. An artificial substrate may be used to support the one or more neuromuscular junctions.

Abstract: The invention discloses a bio-MEMS transducer comprising a cultured myotube and a piezoelectric microcantilever having the myotube attached thereto along a lengthwise extent of said microcantilever. The transducer may include an input/output processor operably connected with said piezoelectric microcantilever to process electrical signals received therefrom and to send electrical signals thereto. The invention may operate as a biosensor wherein the attached myotube contracts on contact with a sensed agent, the myotube contraction deflecting the microcantilever to generate a piezoelectric signal therefrom. The invention may also be used as a biosensor for quantitating physiologic response to an agent by measuring deflection of the cantilever caused by myotube contraction elicited by contact with the agent; and correlating the measurement to effectiveness of the sensed agent in causing a myotube physiologic response.

Abstract: The invention provides a method of co-culturing mammalian muscle cells and mammalian motoneurons. The method comprises preparing one or more carriers coated with a covalently bonded monolayer of trimethoxysilylpropyl diethylenetriamine (DETA); suspending isolated fetal mammalian skeletal muscle cells in serum-free medium according to medium composition 1; suspending isolated fetal mammalian spinal motoneurons in serum-free medium according to medium composition 1; plating the suspended muscle cells onto the one or more carriers at a predetermined density and allowing the muscle cells to attach; plating the suspended motoneurons at a predetermined density onto the one or more carriers and allowing the motoneurons to attach; covering the one or more carriers with a mixture of medium composition 1 and medium composition 2; and incubating the carriers covered in the media mixture.