Abstract: The invention includes a viral vector method and composition comprising transcomplementary replication incompetent viral vectors, preferably adenoviral vectors, which are cotransformed to a recipient cell. The two vectors complement each other and thus allow viral replication, in a synergistic combination which enhances both gene delivery and gene expression of genetic sequences contained within the vector.

Abstract: The present invention relates generally to methods and compositions for the identification of differential protein expression patterns and concomitantly the active genetic regions that are directly or indirectly involved in different tissue types, disease states, or other cellular differences desirable for diagnosis or for targets for drug therapy.

Abstract: A method is described for the rapid identification and isolation of cells based on the presence or absence of an ectopically-expressed N-acetyllactosaminide 3-? Galactosyltransferase (?GT) enzyme for the production of ?Galactosyl-(1,3)Galactosyl (?Gal) epitopes on the surface of ?Gal-negative cells. These cells which are genetically modified to express the ?GT enzyme and ?Gal epitopes on glycosylated lipids and proteins of the cell surface are then labeled via an antibody composition which recognizes and binds the ?Gal epitopes on the cell surface. Cells labeled with the anti-?Gal antibody can be isolated by sorting via fluorescence activated cell sorting (FACS), or by magnetic panning techniques. This method is suitable for the rapid positive or negative selection of ?Gal-positive cells from within a population of ?Gal-negative cells without the need to expose cells to antibiotics for any period of time.

Abstract: A fiberizing apparatus for converting molten material into continuous fibers has an internal support structure to minimize high temperature creep and sagging of a tip plate, the reinforcing structure forming at least about 16 internal cells. The hole pattern in a bushing screen is engineered such that the hole area in each screen area above each of the at least 16 cells controls the amount of molten material flowing into each cell to produce a substantially improved tip plate temperature profile and fiberizing efficiency. The preferred internal support structure is welded to the sidewalls and/or and the top surface of the tip plate and is comprised of a plurality of internal intersecting supports that form various patterns including a diamond shaped structure. The screen used in the preferred bushing has a plurality of diamond shaped hole patterns, some with different hole area per unit screen area than that of other screen areas.