Patents by Inventor James Hoch
James Hoch has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20070298474Abstract: A method for enhancing a host cell's biosynthetic production of 2-KLG is described. Such method comprises selecting a host cell that has an at least partially intracellular synthetic pathway which utilizes 2,5-DKG to produce 2-KLG; increasing the transport of said 2,5-DKG into said host cell while maintaining the integrity of the host cell; culturing the host cell to produce said 2,5-DKG; and producing 2-KLG. The transport of the 2,5-DKG is increased by transforming into the host cell DNA encoding for one or more enzymes transporting the 2,5-DKG into the host cell.Type: ApplicationFiled: July 16, 2007Publication date: December 27, 2007Inventors: Manoj Kumar, Fernando Valle, Veronique Dartois, James Hoch
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Patent number: 7229795Abstract: The invention provides isolated nucleic acid molecules encoding polypeptides having 2,5-DKG permease activity, and oligonucleotides therefrom. The isolated nucleic acid molecules can be expressed in appropriate bacterial cells to enhance the production of 2-KLG, which can subsequently be converted to ascorbic acid. Further provided are isolated polypeptides having 2,5-DKG permease activity, immunogenic peptides therefrom, and antibodies specific therefor. The invention also provides methods of identifying novel 2,5-DKG permeases.Type: GrantFiled: February 25, 2004Date of Patent: June 12, 2007Assignees: Genencor International, Inc., E.P.I. Liquidation Co.Inventors: Veronique A. Dartois, James A. Hoch, Fernando Valle, Manoj Kumar
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Patent number: 7229811Abstract: The invention provides isolated nucleic acid molecules encoding polypeptides having 2,5-DKG permease activity, and oligonucleotides therefrom. The isolated nucleic acid molecules can be expressed in appropriate bacterial cells to enhance the production of 2-KLG, which can subsequently be converted to ascorbic acid. Further provided are isolated polypeptides having 2,5-DKG permease acitivity, immunogenic peptides therefrom, and antibodies specific therefor. The invention also provides methods of identifying novel 2,5-DKG permeases.Type: GrantFiled: August 3, 2001Date of Patent: June 12, 2007Assignees: Genencor International, Inc., E.P.I. Liquidation Co.Inventors: Manoj Kumar, Fernando Valle, Veronique A. Dartois, James A. Hoch
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Publication number: 20060147950Abstract: The invention provides methods of identifying a host cell that encodes a metabolic pathway that converts a precursor molecule into a growth inhibitory compound, by: (a) culturing a population of host cells under conditions that allow expression of the metabolic pathway; (b) contacting the host cells, or an extract thereof, with a population of target cells and the precursor molecule; and (c) identifying a host cell that inhibits growth of the target cells in the presence, but not in the absence, of the precursor molecule, where an identified host cell from step (c) contains a metabolic pathway that converts the precursor molecule into a growth inhibitory compound. Methods of identifying host cells that encode a metabolic pathway that converts a precursor molecule into a desired product compound are also provided. Further. provided are methods of identifying nucleic acid molecules encoding the metabolic pathways.Type: ApplicationFiled: September 9, 2005Publication date: July 6, 2006Inventors: James Hoch, Veronique Dartois
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Publication number: 20050032088Abstract: The invention provides isolated nucleic acid molecules encoding polypeptides having 2,5-DKG permease activity, and oligonucleotides therefrom. The isolated nucleic acid molecules can be expressed in appropriate bacterial cells to enhance the production of 2-KLG, which can subsequently be converted to ascorbic acid. Further provided are isolated polypeptides having 2,5-DKG permease activity, immunogenic peptides therefrom, and antibodies specific therefor. The invention also provides methods of identifying novel 2,5-DKG permeases.Type: ApplicationFiled: February 25, 2004Publication date: February 10, 2005Inventors: Veronique Dartois, James Hoch, Fernando Valle, Manoj Kumar
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Patent number: 6720168Abstract: The invention provides isolated nucleic acid molecules encoding polypeptides having 2,5-DKG permease activity, and oligonucleotides therefrom. The isolated nucleic acid molecules can be expressed in appropriate bacterial cells to enhance the production of 2-KLG, which can subsequently be converted to ascorbic acid. Further provided are isolated polypeptides having 2,5-DKG permease activity, immunogenic peptides therefrom, and antibodies specific therefor. The invention also provides methods of identifying novel 2,5-DKG permeases.Type: GrantFiled: August 3, 2001Date of Patent: April 13, 2004Assignee: Genencor International, Inc.Inventors: Veronique A. Dartois, James A. Hoch, Fernando Valle, Manoj Kumar
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Publication number: 20040029234Abstract: A method for enhancing a host cell's biosynthetic production of 2-KLG is described. Such method comprises selecting a host cell that has an at least partially intracellular synthetic pathway which utilizes 2,5-DKG to produce 2-KLG; increasing the transport of said 2,5-DKG into said host cell while maintaining the integrity of the host cell; culturing the host cell to produce said 2,5-DKG; and producing 2-KLG. The transport of the 2,5-DKG is increased by transforming into the host cell DNA encoding for one or more enzymes transporting the 2,5-DKG into the host cell.Type: ApplicationFiled: July 18, 2003Publication date: February 12, 2004Inventors: Manoj Kumar, Fernando Valle, Veronique A. Dartois, James A. Hoch
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Publication number: 20040030113Abstract: The invention provides isolated nucleic acid molecules encoding polypeptides having 2,5-DKG permease activity, and oligonucleotides therefrom. The isolated nucleic acid molecules can be expressed in appropriate bacterial cells to enhance the production of 2-KLG, which can subsequently be converted to ascorbic acid. Further provided are isolated polypeptides having 2,5-DKG permease acitivity, immunogenic peptides therefrom, and antibodies specific therefor. The invention also provides methods of identifying novel 2,5-DKG permeases.Type: ApplicationFiled: August 18, 2003Publication date: February 12, 2004Inventors: Manoj Kumar, Fernando Valle, Veronique A. Dartois, James A. Hoch
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Publication number: 20030073140Abstract: The present invention relates in part to methods for screening for novel enzymatic pathways in environmental samples using metabolic selection strategies, and the isolation of the genes and proteins that make up these pathways.Type: ApplicationFiled: April 25, 2000Publication date: April 17, 2003Inventors: James Hoch, Veronique Dartois
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Publication number: 20030068807Abstract: The invention provides methods of identifying a host cell that encodes a metabolic pathway that converts a precursor molecule into a growth inhibitory compound, by: (a) culturing a population of host cells under conditions that allow expression of the metabolic pathway; (b) contacting the host cells, or an extract thereof, with a population of target cells and the precursor molecule; and (c) identifying a host cell that inhibits growth of the target cells in the presence, but not in the absence, of the precursor molecule, where an identified host cell from step (c) contains a metabolic pathway that converts the precursor molecule into a growth inhibitory compound. Methods of identifying host cells that encode a metabolic pathway that converts a precursor molecule into a desired product compound are also provided. Further provided are methods of identifying nucleic acid molecules encoding the metabolic pathways.Type: ApplicationFiled: July 31, 2002Publication date: April 10, 2003Applicant: Microgenomics, Inc.Inventors: James Hoch, Veronique Dartois
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Publication number: 20020120119Abstract: The invention provides isolated nucleic acid molecules encoding polypeptides having 2,5-DKG permease activity, and oligonucleotides therefrom. The isolated nucleic acid molecules can be expressed in appropriate bacterial cells to enhance the production of 2-KLG, which can subsequently be converted to ascorbic acid. Further provided are isolated polypeptides having 2,5-DKG permease activity, immunogenic peptides therefrom, and antibodies specific therefor. The invention also provides methods of identifying novel 2,5-DKG permeases.Type: ApplicationFiled: August 3, 2001Publication date: August 29, 2002Inventors: Veronique A. Dartois, James A. Hoch, Fernando Valle, Manoj Kumar
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Patent number: 6368793Abstract: The present invention relates in part to methods for screening for novel enzymatic pathways in environmental samples using metabolic selection strategies, and the isolation of the genes and proteins that make up these pathways.Type: GrantFiled: October 14, 1998Date of Patent: April 9, 2002Assignee: MicroGenomics, Inc.Inventors: James Hoch, Veronique Dartois
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Patent number: 6043045Abstract: The present invention relates to screening methods for the identification of compounds and compositions useful as novel antibiotics and antibacterial agents. In particular, the present invention relates to methods utilizing two-component regulatory switches which includes regulatory switches comprising a prokaryotic enzyme. Histidine protein kinase is a regulatory switch that is activated to autophosphorylate by a signal transduction mechanism. The invention also relates to methods of identifying inhibitors of enzyme activity particularly in bacterial cells. A high-throughput assay system useful in the large-scale screening of protein kinase inhibitors is also provided. The invention further provides the phosphorylation of SpoOF including a histidine.Type: GrantFiled: May 29, 1998Date of Patent: March 28, 2000Assignee: The Scripps Research InstituteInventors: James A. Hoch, Shaoming Huang
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Patent number: 5848260Abstract: To simulate physical processes, state vectors for each of multiple voxels are stored in a memory along with a representation for each of multiple facets that are sized and oriented independently of the size and orientation of the voxels and, in combination, represent one or more surfaces. Each state vector includes multiple entries, each of which corresponds to a number of elements at a particular momentum state of multiple possible momentum states at a voxel. Interaction operations that model interactions between elements of different momentum states are performed on the state vectors, and surface interaction operations that model interactions between a facet and elements at one or more voxels near the facet are performed on the representations of facets. Finally, move operations that reflect movement of elements to new voxels are performed on the state vectors.Type: GrantFiled: December 1, 1995Date of Patent: December 8, 1998Assignee: Exa CorporationInventors: Hudong Chen, David Lawerence Hill, James Hoch, Kim Molvig, Christopher M. Teixeira, Kenneth R. Traub
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Patent number: 5747276Abstract: The present invention relates to screening methods for the identification of compounds and compositions useful as novel antibiotics and antibacterial agents. In particular, the present invention relates to methods utilizing two-component regulatory switches, for example, those comprising a prokaryotic enzyme such as histidine protein kinase that is activated to autophosphorylate by a signal transduction mechanism. The invention also relates to methods of identifying inhibitors of enzyme activity, particularly in bacterial cells. In particular, a high-throughput assay system useful in the large-scale screening of protein kinase inhibitors is disclosed.Type: GrantFiled: September 15, 1995Date of Patent: May 5, 1998Assignee: The Scripps Research InstituteInventors: James A. Hoch, Shaoming Huang