Abstract: Rationally-designed LAGLIDADG meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research.

Abstract: Rationally-designed LAGLIDADG meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research.

Abstract: Rationally-designed LAGLIDADG meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research.

Abstract: Rationally-designed LAGLIDADG meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research.

Abstract: Rationally-designed LAGLIDADG meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research.

Abstract: Rationally-designed LAGLIDADG meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research.

Abstract: Rationally-designed LAGLIDADG (SEQ ID NO: 37) meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research.

Abstract: Methods of cleaving double-stranded DNA that can be recognized and cleaved by a rationally-designed, I-CreI-derived meganuclease are provided. Also provided are recombinant nucleic acids, cells, and organisms containing such recombinant nucleic acids, as well as cells and organisms produced using such meganucleases. Also provided are methods of conducting a custom-designed, I-CreI-derived meganuclease business.

Abstract: Targeted transcriptional effectors (transcription activators and transcription repressors) derived from meganucleases are described. Also described are nucleic acids encoding same, and methods of using same to regulate gene expression. The targeted transcriptional effectors can comprise (i) a meganuclease DNA-binding domain lacking endonuclease cleavage activity that binds to a target recognition site; and (ii) a transcription effector domain.

Abstract: Rationally-designed LAGLIDADG meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research.

Abstract: Disclosed are rationally-designed, non-naturally-occurring meganucleases in which a pair of enzyme subunits having specificity for different recognition sequence half-sites are joined into a single polypeptide to form a functional heterodimer with a non-palindromic recognition sequence. The invention also relates to methods of producing such meganucleases, and methods of producing recombinant nucleic acids and organisms using such meganucleases.

Abstract: The invention relates to methods for the production of genetically modified plants using engineered meganucleases and elevated temperature and to genetically modified plants produced by such methods.

Abstract: The present invention relates, in general, to protein modifications and, in particular, to a method of effecting site-specific labeling of proteins with covalently coupled reporter groups. The invention further relates to a method of effecting orientation-specific immobilization of proteins on a solid surface. The invention also relates to products produced by such methods.

Abstract: A method for configuring a digital overcurrent relay to provide a plurality of selectable time overcurrent characteristic responses corresponding to a plurality of target curves. According to one embodiment, a template curve is selected from the plurality of target curves, and a scaling factor is generated for each remaining target curve, wherein the relay comprises means for generating approximated curves from a given template curve and a given scaling factor. It is determined whether each approximated curve is within a threshold amount of total error with respect to its corresponding target curve. If any approximated curves are above the threshold amount, then a further template is selected from the plurality of target curves, and scaling factors are again generated and adjusted for the further template curve, until no approximated curves are above the threshold amount.

Abstract: A remote closing operator is positioned within a circuit breaker enclosure to allow remote operation of the circuit breaker operating mechanism to open and close the circuit breaker contacts. A pair of solenoids are employed to control the state of the contact closing springs. The first solenoid is energized to initially release the contact closing springs and the second solenoid is energized to interrupt the flow of current to the first solenoid.

Abstract: An endotracheal device for passing fluids into or out of the body includes a tube member with a laser reflective material in the form of a flexible sheath that is covered by a flexible non-metallic protective covering. The laser reflective material is preferably metallic and can be formed of aluminum, copper, silver or stainless steel. Flexibility of the laser reflective material is obtained by applying the sheath to the tube member in the form of a spiral or in a corrugated form. A protective covering is provided over the laser reflective material and is preferably formed of a polytetrafluoroethylene material. The protective covering, coating or tubular wrapping is applied in a manner which enables such covering to rupture or break when a laser beam strikes the tube. The area of breakage of the protective covering exposes the underlying laser reflective material thereby enabling the laser beam to be reflected away from the tubular member.

Abstract: A microscope drape fabricated from a very thin, transparent heat-resistant plastic film which is adapted to completely house an operating microscope including its support arms, is provided with an improved means for securing the tubular ocular housing extension of the drape to the oculars of the microscope which consists of a thin malleable strip attached to the ocular housing which permits the ocular housing to be secured to the microscope by crimping and folding over the metal strip after positioning the ocular housing on an ocular of a microscope.

Abstract: A cable and battery terminal cleaner particularly adapted for cleaning the terminals of a side post storage battery and the cable ends attached thereto includes a substantially cylindrically shaped housing. An annular row of wire bristles extends from one end of the housing. Within the row of bristles and extending outwardly beyond the ends of the bristles is a pilot pin which is adapted to fit within the bolt hole of a terminal to act as a guide for rotary movement of the cleaner. The pin may also be pushed inwardly against a spring so that the end of a bolt may enter the opening at the end of the housing to also act as a guide when a cable end is being cleaned. A socket is located on the remote wall of the housing for connecting the entire cleaner to a rotary power tool if desired.