Abstract: Methods for preparing strand-specific sequencing libraries of oligonucleotides using an RNA polymerase promoter to re-transcribe antisense cDNA which has been reverse transcribed from mRNA are provided. The transcription step linearly amplifies sRNA prior to production of double-stranded cDNA to be sequenced and may be sufficient to eliminate the conventional PCR amplification step prior to sequencing. The methods incorporate anchor sequences, amplification sequences and other sequences required for a particular sequencing system or reaction by hybridization and extension of primers, and transcription of RNA, rather than ligation, thus reducing the number of steps and the time required for sample preparation for sequencing of RNA. Use of primer hybridization and transcription reactions in the methods also results in a library that exhibits reduced 3? sequence bias.

Abstract: Compositions and methods are provided for amplifying nucleic acid molecules. The nucleic acid molecules can be used in various research and diagnostic applications, such as gene expression studies involving nucleic acid microarrays.

Abstract: Methods and kits are provided for the production and use in microarray assays of labeled miRNA molecules and labeled cDNA molecules complementary to miRNA molecules.

Abstract: Compositions and methods are provided for amplifying nucleic acid molecules. The nucleic acid molecules can be used in various research and diagnostic applications, such as gene expression studies involving nucleic acid microarrays.

Abstract: Compositions and methods are provided for amplifying nucleic acid molecules. The nucleic acid molecules can be used in various research and diagnostic applications, such as gene expression studies involving nucleic acid microarrays.

Abstract: Methods, reagents and kits are provided for the production and use in detection assays of labeled nucleic acid molecules wherein a labeling molecule is attached directly to the 3? end of the nucleic acid molecules.

Abstract: Methods and kits are provided for performing multiple rounds of sense RNA synthesis. The sense RNA molecules can be used in various research and diagnostic applications, such as gene expression studies involving nucleic acid microarrays.

Abstract: Methods and kits are provided for performing multiple rounds of sense RNA synthesis. The sense RNA molecules can be used in various research and diagnostic applications, such as gene expression studies involving nucleic acid microarrays.

Abstract: Methods, reagents and kits are provided for the production and use in detection assays of labeled nucleic acid molecules wherein a labeling molecule is attached directly to the 3? end of the nucleic acid molecules.

Abstract: A method for synthesis of nucleic acids, with particular use for synthesis of cDNA. The method allows application of the cDNA to a microarray without the need for concentration or purification of the cDNA post-cDNA synthesis.

Abstract: Compositions and methods are provided for amplifying nucleic acid molecules. The nucleic acid molecules can be used in various research and diagnostic applications, such as gene expression studies involving nucleic acid microarrays.

Abstract: Compositions and methods are provided for amplifying nucleic acid molecules. The nucleic acid molecules can be used in various research and diagnostic applications, such as gene expression studies involving nucleic acid microarrays.

Abstract: Methods and kits are provided for performing multiple rounds of sense RNA synthesis. The sense RNA molecules can be used in various research and diagnostic applications, such as gene expression studies involving nucleic acid microarrays.

Abstract: A method of constructing a highly labeled linear polymeric molecule for use in any desired application, and linear polymeric molecules of such structure. The polymeric molecule is a nucleic acid constructed from a large number of one or more types of monomeric oligonucleotide units that are attached together to form an extended strand. Within each polymer, at least one type of monomeric unit is provided which is bound to or designed to bind to a label moiety, providing a polymer with a large number of repeat sequences designed for labeling purposes, resulting in extremely effective signal carrying molecule of considerable versatility.

Abstract: Methods and kits are provided for the production and use in microarray assays of labeled miRNA molecules and labeled cDNA molecules complementary to miRNA molecules.

Abstract: The present invention is directed to a method for determining the presence of a specific nucleotide sequence in a cDNA reagent of a target sample utilizing a capture reagent having at least one first arm containing a label capable of emitting a detectable signal and at least one second arm having a nucleotide sequence complementary to a capture sequence attached to the cDNA reagent on a microarray through temperature cycling.