Patents by Inventor James L. Hartley

James L. Hartley has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20130059342
    Abstract: The present invention relates to compositions and methods for use in recombinational cloning of nucleic acid molecules. In particular, the invention relates to nucleic acid molecules encoding one or more recombination sites or portions thereof, to nucleic acid molecules comprising one or more of these recombination site nucleotide sequences and optionally comprising one or more additional physical or functional nucleotide sequences. The invention also relates to vectors comprising nucleic acid molecules of the invention, to host cells comprising vectors or nucleic acid molecules of the invention, to methods of producing polypeptides using nucleic acid molecules of the invention, and to polypeptides encoded by these nucleic acid molecules or produced by methods of the invention. The invention also relates to the use of these compositions in methods for recombinational cloning of nucleic acids, in vitro and in vivo, to provide chimeric DNA molecules that have particular characteristics and/or DNA segments.
    Type: Application
    Filed: June 28, 2012
    Publication date: March 7, 2013
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: James L. HARTLEY, Michael A. Brasch, Gary F. Temple, David Cheo
  • Publication number: 20130004996
    Abstract: The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different.
    Type: Application
    Filed: August 26, 2011
    Publication date: January 3, 2013
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Jonathan D. CHESNUT, John Carrino, Louis Leong, Knut Madden, Martin A. G. Gleeson, James Fan, Michael A. Brasch, David Cheo, James L. Hartley, Devon R.N. Byrd, Gary F. Temple
  • Publication number: 20120183999
    Abstract: The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites with unique specificity. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. Such molecules and/or compounds or combinations of such molecules and/or compounds can also be bound through recombination to various structures or supports according to the invention.
    Type: Application
    Filed: November 23, 2011
    Publication date: July 19, 2012
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: David CHEO, Michael A. Brasch, Gary F. Temple, James L. Hartley, Devon R.N. Byrd
  • Patent number: 8148302
    Abstract: The invention provides a microarray and methods for producing a protein microarray. The array comprises multiple nucleic acid molecules immobilized on a substrate, each comprising (i) a protein-binding domain and (ii) a nucleic acid sequence encoding a fusion protein comprising a polypeptide of interest and a DNA-binding protein that binds the protein-binding domain, and one or more fusion proteins produced from the multiple nucleic acid molecules. Each fusion protein is immobilized on the substrate via binding to a nucleic acid sequence comprising the protein-binding domain present on the nucleic acid molecule from which the fusion protein is produced or on the substrate. The invention also provides a method of analyzing protein interactions with, for example, other proteins, lipids and drugs.
    Type: Grant
    Filed: October 19, 2005
    Date of Patent: April 3, 2012
    Assignee: The United States of America as represented by the Department of Health and Human Services
    Inventors: Deb K Chatterjee, Kalavathy Sitaraman, James L Hartley, Cassio Baptista, David J Munroe
  • Publication number: 20110287489
    Abstract: Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s).
    Type: Application
    Filed: November 24, 2010
    Publication date: November 24, 2011
    Applicant: Life Technologies Corporation
    Inventors: James L. HARTLEY, Michael A. Brasch
  • Publication number: 20110281767
    Abstract: The present invention provides materials and methods for the utilization of the specific interaction of replication termination sequences with their binding proteins in molecular biology applications.
    Type: Application
    Filed: January 28, 2011
    Publication date: November 17, 2011
    Applicant: Life Technologies Corporation
    Inventors: Devon R.N. Byrd, Alice Young, James L. Hartley
  • Publication number: 20110275541
    Abstract: The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites with unique specificity. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. Such molecules and/or compounds or combinations of such molecules and/or compounds can also be bound through recombination to various structures or supports according to the invention.
    Type: Application
    Filed: January 8, 2008
    Publication date: November 10, 2011
    Applicant: INVITROGEN CORPORATION
    Inventors: David Cheo, Michael A. Brasch, Gary F. Temple, James L. Hartley, Devon R.N. Byrd
  • Patent number: 8030066
    Abstract: The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different.
    Type: Grant
    Filed: December 18, 2006
    Date of Patent: October 4, 2011
    Assignee: Life Technologies Corporation
    Inventors: Jonathan D. Chesnut, John Carrino, Louis Leong, Knut Madden, Martin Gleeson, James Fan, Michael Brasch, David Cheo, James L. Hartley, Devon R. Byrd, Gray F. Temple
  • Publication number: 20110132760
    Abstract: The invention relates to a nucleic acid marker ladder which is a restriction endonuclease digest, wherein a nucleic acid restriction endonuclease digest is a collection of nucleic acid fragments resulting from complete digestion of one or more nucleic acids by one or more restriction endonucleases; the restriction endonuclease digest contains at least 3 fragments; and the size of the fragments in base pairs is a multiple of an integer, wherein the integer is 10 or more.
    Type: Application
    Filed: December 7, 2010
    Publication date: June 9, 2011
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventor: JAMES L. HARTLEY
  • Publication number: 20110033920
    Abstract: The present invention relates generally to compositions and methods for use in recombinational cloning of nucleic acid molecules. In particular, the invention relates to nucleic acid molecules encoding one or more recombination sites or portions thereof, to nucleic acid molecules comprising one or more of these recombination site nucleotide sequences and optionally comprising one or more additional physical or functional nucleotide sequences. The invention also relates to vectors comprising the nucleic acid molecules of the invention, to host cells comprising the vectors or nucleic acid molecules of the invention, to methods of producing polypeptides using the nucleic acid molecules of the invention, and to polypeptides encoded by these nucleic acid molecules or produced by the methods of the invention. The invention also relates to antibodies that bind to one or more polypeptides of the invention or epitopes thereof.
    Type: Application
    Filed: December 29, 2009
    Publication date: February 10, 2011
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: James L. Hartley, Michael A. Brasch, Gary F. Temple, David Cheo
  • Publication number: 20100267120
    Abstract: Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s).
    Type: Application
    Filed: July 6, 2009
    Publication date: October 21, 2010
    Inventors: James L. Hartley, Michael A. Brasch, Gary F. Temple, Donna K. Fox
  • Publication number: 20100267119
    Abstract: Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s).
    Type: Application
    Filed: July 6, 2009
    Publication date: October 21, 2010
    Inventors: James L. Hartley, Michael A. Brasch, Gary F. Temple, Donna K. Fox
  • Publication number: 20100267118
    Abstract: Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s).
    Type: Application
    Filed: July 2, 2009
    Publication date: October 21, 2010
    Applicant: LIFE TECHNOLOGIES CORPORATION, a Delaware Corporation
    Inventors: James L. Hartley, Michael A. Brasch, Gary F. Temple, Donna K. Fox
  • Patent number: 7687247
    Abstract: This invention relates to a method of incorporating an exo-sample nucleotide into the amplified product strands resulting from a nucleic acid amplification process. Once the product strands have been obtained and analyzed (e.g., by hybridization, Southern blot, etc.), the exo-sample strands can be selectively destroyed by acting on the incorporated exo-sample nucleotide. Two embodiments are presented. In a first embodiment, the exo-sample nucleotide is incorporated by carrying out the amplification to reaction in the presence of an excess of exo-sample nucleotide tri-phosphate. In a second embodiment, the exo-sample nucleotide is incorporated by carrying out the amplification reaction in the presence of an oligonucleotide which has, as part of its sequence, one or more exo-sample nucleotides.
    Type: Grant
    Filed: January 19, 1994
    Date of Patent: March 30, 2010
    Assignee: Life Technologies Corporation
    Inventors: James L. Hartley, Mark Berninger
  • Patent number: 7670823
    Abstract: The present invention relates generally to compositions and methods for use in recombinational cloning of nucleic acid molecules. In particular, the invention relates to nucleic acid molecules encoding one or more recombination sites or portions thereof, to nucleic acid molecules comprising one or more of these recombination site nucleotide sequences and optionally comprising one or more additional physical or functional nucleotide sequences. The invention also relates to vectors comprising the nucleic acid molecules of the invention, to host cells comprising the vectors or nucleic acid molecules of the invention, to methods of producing polypeptides using the nucleic acid molecules of the invention, and to polypeptides encoded by these nucleic acid molecules or produced by the methods of the invention. The invention also relates to antibodies that bind to one or more polypeptides of the invention or epitopes thereof.
    Type: Grant
    Filed: March 2, 2000
    Date of Patent: March 2, 2010
    Assignee: Life Technologies Corp.
    Inventors: James L. Hartley, Michael A. Brasch, Gary F. Temple, David Cheo
  • Publication number: 20090186387
    Abstract: Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s).
    Type: Application
    Filed: October 8, 2008
    Publication date: July 23, 2009
    Applicant: INVITROGEN CORPORATION
    Inventors: James L. Hartley, Michael Brasch
  • Publication number: 20090186386
    Abstract: Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s).
    Type: Application
    Filed: September 19, 2008
    Publication date: July 23, 2009
    Applicant: INVITROGEN CORPORATION
    Inventors: JAMES L. HARTLEY, MICHAEL A. BRASCH, GARY F. TEMPLE, DONNA K. FOX
  • Publication number: 20090186385
    Abstract: Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s).
    Type: Application
    Filed: September 9, 2008
    Publication date: July 23, 2009
    Applicant: INVITROGEN CORPORATION
    Inventors: James L. Hartley, Michael Brasch, Gary F. Temple, Donna K. Fox
  • Publication number: 20090149640
    Abstract: The present invention provides nucleic acid molecules which may be used as standards for estimating the size (in base pairs) and mass of linear, double-stranded or single-stranded nucleic acid molecules separated by size. The nucleic acid molecules of the invention may be DNA molecules, RNA molecules or DNA/RNA hybrid molecules, and may be double-stranded or single-stranded. The invention also provides methods for producing nucleic acid sizing ladders from these nucleic acid molecules, ladders produced by such methods, and methods for estimating the size and mass of nucleic acid molecules by comparison to these nucleic acid sizing ladders.
    Type: Application
    Filed: November 10, 2008
    Publication date: June 11, 2009
    Applicant: INVITROGEN CORPORATION
    Inventors: A-Li W. Hu, James L. Hartley, Heather J. Jordan
  • Publication number: 20090111112
    Abstract: The invention relates to a nucleic acid marker ladder which is a restriction endonuclease digest, wherein a nucleic acid restriction endonuclease digest is a collection of nucleic acid fragments resulting from complete digestion of one or more nucleic acids by one or more restriction endonucleases; the restriction endonuclease digest contains at least 3 fragments; and the size of the fragments in base pairs is a multiple of an integer, wherein the integer is 10 or more.
    Type: Application
    Filed: September 25, 2008
    Publication date: April 30, 2009
    Applicant: INVITROGEN CORPORATION
    Inventor: James L. HARTLEY