Patents by Inventor James L. Schram
James L. Schram has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11016095Abstract: Disclosed herein are diagnostic assays using surface enhanced Raman spectroscopy (SERS)-active particles, including liquid-based assays; magnetic capture assays; microparticle-nanoparticle satellite structures for signal amplification in an assay; composite SERS-active particles useful for enhanced detection of targets; and sample tubes and processes for using the same.Type: GrantFiled: September 22, 2017Date of Patent: May 25, 2021Assignee: BECTON DICKINSON AND COMPANYInventors: Kristin Weidemaier, Christian Sandmann, William Shannon Dillmore, James L. Schram, W. William Stewart, Robert E. Pearson, Helen Hsieh, Steven Keith, Rajendra R. Bhat, Andrea Liebmann-Vinson, Adam Craig Curry, Alexander G. Lastovich
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Publication number: 20180059118Abstract: Disclosed herein are diagnostic assays using surface enhanced Raman spectroscopy (SERS)-active particles, including liquid-based assays; magnetic capture assays; microparticle-nanoparticle satellite structures for signal amplification in an assay; composite SERS-active particles useful for enhanced detection of targets; and sample tubes and processes for using the same.Type: ApplicationFiled: September 22, 2017Publication date: March 1, 2018Applicant: BECTON DICKINSON AND COMPANYInventors: Kristin Weidemaier, Christian Sandmann, William Shannon Dillmore, James L. Schram, W. William Stewart, Robert E. Pearson, Helen Hsieh, Steven Keith, Rajendra R. Bhat, Andrea Liebmann-Vinson, Adam Craig Curry, Alexander G. Lastovich
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Patent number: 9823253Abstract: Disclosed herein are diagnostic assays using surface enhanced Raman spectroscopy (SERS)-active particles, including liquid-based assays; magnetic capture assays; microparticle-nanoparticle satellite structures for signal amplification in an assay; composite SERS-active particles useful for enhanced detection of targets; and sample tubes and processes for using the same.Type: GrantFiled: March 20, 2008Date of Patent: November 21, 2017Assignee: Becton, Dickinson and CompanyInventors: Kristin Weidemaier, Christian Sandmann, W. Shannon Dillmore, James L. Schram, W. William Stewart, Robert E. Pearson, Helen Hsieh, Steven Keith, Rajendra R. Bhat, Andrea Liebmann-Vinson, Adam Craig Curry, Alexander G. Lastovich
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Publication number: 20110275061Abstract: Disclosed herein are diagnostic assays using surface enhanced Raman spectroscopy (SERS)-active particles, including liquid-based assays; magnetic capture assays; microparticle-nanoparticle satellite structures for signal amplification in an assay; composite SERS-active particles useful for enhanced detection of targets; and sample tubes and processes for using the same.Type: ApplicationFiled: March 20, 2008Publication date: November 10, 2011Inventors: Kristin Weidemaier, Christian Sandmann, W. Shannon Dillmore, James L. Schram, W. William Stewart, Robert E. Pearson, Helen Hsieh, Steven Keith, Rajendra R. Bhat, Andrea Liebmann-Vinson, Adam Craig Curry, Alexander G. Lastovich
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Patent number: 6054279Abstract: Single-stranded signal primers are modified by linkage to two dyes which form a donor/acceptor dye pair. The two dyes are positioned in sufficiently close spatial proximity on the signal primer that the fluorescence of the first dye is quenched by the second dye. The signal primer may further comprise a restriction endonuclease recognition site (RERS) between the two dyes. As the signal primer is initially single-stranded and remains single-stranded in the absence of target, the restriction endonuclease recognition site is not cleavable or nickable by the restriction endonuclease. In the presence of target, however, signal primer and the restriction endonuclease recognition site are rendered double-stranded and cleavable or nickable by the restriction endonuclease. Cleavage or nicking separates the two dyes and a change in fluorescence due to decreased quenching is detected as an indication of the presence of the target sequence or of target sequence amplification.Type: GrantFiled: July 20, 1998Date of Patent: April 25, 2000Assignee: Becton Dickinson and CompanyInventors: James G. Nadeau, J. Bruce Pitner, James L. Schram, C. Preston Linn, Glenn P. Vonk, G. Terrance Walker
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Patent number: 5958700Abstract: A detector oligonucleotide having a sequence which forms an intramolecularly base-paired secondary structure is described for use in detecting nucleic acid target sequences and target sequence amplification. The detector oligonucleotide is further modified by linkage to two dyes which form a donor/acceptor dye pair. The two dyes are positioned on the detector oligonucleotide such that they are in close spatial proximity in the base-paired, folded secondary structure, thereby causing quenching of donor fluorescence. The detector oligonucleotide may optionally further comprise a restriction endonuclease recognition site (RERS) which remains partially or entirely single-stranded in the base-paired secondary structure. The RERS is flanked by the two dyes. In the presence of target, the base-paired secondary structure is unfolded or linearized, increasing the distance between the donor and acceptor dyes and causing a change in fluorescence of the donor and/or the acceptor.Type: GrantFiled: January 26, 1999Date of Patent: September 28, 1999Assignee: Becton, Dickinson and CompanyInventors: James G. Nadeau, J. Bruce Pitner, C. Preston Linn, James L. Schram
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Patent number: 5928869Abstract: A detector oligonucleotide having a sequence which forms an intramolecularly base-paired secondary structure is described for use in detecting nucleic acid target sequences and target sequence amplification. The detector oligonucleotide is further modified by linkage to two dyes which form a donor/acceptor dye pair. The two dyes are positioned on the detector oligonucleotide such that they are in close spatial proximity in the base-paired, folded secondary structure, thereby causing quenching of donor fluorescence. The detector oligonucleotide may optionally further comprise a restriction endonuclease recognition site (RERS) which remains partially or entirely single-stranded in the base-paired secondary structure. The RERS is flanked by the two dyes. In the presence of target, the base-paired secondary structure is unfolded or linearized, increasing the distance between the donor and acceptor dyes and causing a change in fluorescence of the donor and/or the acceptor.Type: GrantFiled: May 30, 1997Date of Patent: July 27, 1999Assignee: Becton, Dickinson and CompanyInventors: James G. Nadeau, J. Bruce Pitner, C. Preston Linn, James L. Schram
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Patent number: 5919630Abstract: Single-stranded signal primers are modified by linkage to two dyes which form a donor/acceptor dye pair. The two dyes are positioned in sufficiently close spatial proximity on the signal primer that the fluorescence of the first dye is quenched by the second dye. The signal primer may further comprise a restriction endonuclease recognition site (RERS) between the two dyes. As the signal primer is initially single-stranded and remains single-stranded in the absence of target, the restriction endonuclease recognition site is not cleavable or nickable by the restriction endonuclease. In the presence of target, however, signal primer and the restriction endonuclease recognition site are rendered double-stranded and cleavable or nickable by the restriction endonuclease. Cleavage or nicking separates the two dyes and a change in fluorescence due to decreased quenching is detected as an indication of the presence of the target sequence or of target sequence amplification.Type: GrantFiled: November 4, 1998Date of Patent: July 6, 1999Assignee: Becton, Dickinson and CompanyInventors: James G. Nadeau, J. Bruce Pitner, James L. Schram, C. Preston Linn, Glenn P. Vonk, G. Terrance Walker
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Patent number: 5846726Abstract: Single-stranded signal primers are modified by linkage to two dyes which form a donor/acceptor dye pair. The two dyes are positioned in sufficiently close spatial proximity on the signal primer that the fluorescence of the first dye is quenched by the second dye. The signal primer may further comprise a restriction endonuclease recognition site (RERS) between the two dyes. As the signal primer is initially single-stranded and remains single-stranded in the absence of target, the restriction endonuclease recognition site is not cleavable or nickable by the restriction endonuclease. In the presence of target, however, signal primer and the restriction endonuclease recognition site are rendered double-stranded and cleavable or nickable by the restriction endonuclease. Cleavage or nicking separates the two dyes and a change in fluorescence due to decreased quenching is detected as an indication of the presence of the target sequence or of target sequence amplification.Type: GrantFiled: May 13, 1997Date of Patent: December 8, 1998Assignee: Becton, Dickinson and CompanyInventors: James G. Nadeau, J. Bruce Pitner, James L. Schram, C. Preston Linn, Glenn P. Vonk, G. Terrance Walker
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Patent number: 5811269Abstract: Primers and methods for adapter-mediated multiplex amplification of the IS6110 insertion element of the Mycobacterium tuberculosis (M.tb) complex and a 16S rDNA target common to essentially all mycobacteria are described. In certain embodiments, the primers are optimized for efficient multiplex amplification in thermophilic SDA. The multiplex Strand Displacement Amplification methods of the invention are capable, in a single amplification reaction, of simultaneously identifying M. tuberculosis and providing a screen for substantially all of the clinically relevant species of mycobacteria. Also disclosed are internal control sequences designed for coamplification with the two targets, allowing assessment of amplification efficiency and/or quantitation of the targets.Type: GrantFiled: April 30, 1996Date of Patent: September 22, 1998Assignee: Becton, Dickinson and CompanyInventors: James G. Nadeau, Cheryl H. Dean, James L. Schram, Deborah R. Howard, Margaret S. Dey, David J. Wright
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Patent number: 5736365Abstract: Primers and methods for adapter-mediated multiplex amplification of the IS6110 insertion element of Mycobacterium tuberculosis (M.tb) and the 16S ribosomal gene of Mycobacterium tuberculosis, useful for simultaneously detecting and/or identifying species of the M. tuberculosis complex and other clinically relevant Mycobacterium species. Multiplex Strand Displacement Amplification (SDA) is used in a single amplification reaction which is capable of simultaneously identifying M. tuberculosis and providing a screen for substantially all of the clinically relevant species of Mycobacteria. Also disclosed are methods for adapter-mediated multiplex amplification of multiple target sequences and a single internal control sequence for determination of sample efficacy or quantitation of the targets.Type: GrantFiled: August 29, 1996Date of Patent: April 7, 1998Assignee: Becton, Dickinson and CompanyInventors: George Terrance Walker, James G. Nadeau, Patricia Anne Spears, Colleen M. Nycz, Daryl Dee Shank, James L. Schram, Stewart Russel Jurgensen
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Patent number: 5681705Abstract: Amplification primers and methods are disclosed for complex-specific amplification of target sequences in the dnaJ genes of the Mycobacterium Avium Complex species. Also provided are assay probes for detection of the amplification products and/or identification of the MAC species which is present.Type: GrantFiled: August 28, 1995Date of Patent: October 28, 1997Inventors: James L. Schram, James G. Nadeau, Cheryl H. Dean
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Patent number: 5561044Abstract: Primers and methods for adapter-mediated multiplex amplification of the IS6110 insertion element of Mycobacterium tuberculosis (M.tb) and the 16S ribosomal gene of Mycobacterium tuberculosis, useful for simultaneously detecting and/or identifying species of the M. tuberculosis complex and other clinically relevant Mycobacterium species. Multiplex Strand Displacement Amplification (SDA) is used in a single amplification reaction which is capable of simultaneously identifying M. tuberculosis and providing a screen for substantially all of the clinically relevant species of Mycobacteria. Also disclosed are methods for adapter-mediated multiplex amplification of multiple target sequences and a single internal control sequence for determination of sample efficacy or quantitation of the targets.Type: GrantFiled: March 3, 1995Date of Patent: October 1, 1996Assignee: Becton, Dickinson and CompanyInventors: George T. Walker, James G. Nadeau, Patricia A. Spears, Colleen M. Nycz, Daryl D. Shank, James L. Schram, Stewart R. Jurgensen
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Patent number: 5536649Abstract: Methods for inactivating contaminating amplicons in isothermal nucleic acid amplification reactions such as SDA. Uracil is incorporated into the amplicons produced by amplification in the place of thymine (T) using novel SDA reaction conditions. If these amplicons contaminate a subsequent amplification reaction, they may be inactivated as templates (i.e., rendered unamplifiable) by treatment with UDG. As isothermal amplification does not involve elevated temperatures, the UDG may be inactivated during the subsequent amplification of specific target sequences by inclusion of the UDG inhibitor protein Ugi. Incorporation of dU has unexpectedly been found to enhance the amplification power of SDA as compared to conventional SDA reactions. The methods may also be used to detect UDG activity in reagents or samples.Type: GrantFiled: July 29, 1994Date of Patent: July 16, 1996Assignee: Becton, Dickinson and CompanyInventors: Melinda S. Fraiser, George T. Walker, James L. Schram
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Patent number: 5470723Abstract: Primers and methods for adapter-mediated multiplex amplification of the IS6110 insertion element of Mycobacterium tuberculosis (M.tb) and the 16S ribosomal gene of Mycobacterium tuberculosis, useful for simultaneously detecting and/or identifying species of the M. tuberculosis complex and other clinically relevant Mycobacterium species. Multiplex Strand Displacement Amplification (SDA) is used in a single amplification reaction which is capable of simultaneously identifying M. tuberculosis and providing a screen for substantially all of the clinically relevant species of Mycobacteria. Also disclosed are methods for adapter-mediated multiplex amplification of multiple target sequences and a single internal control sequence for determination of sample efficacy or quantitation of the targets.Type: GrantFiled: August 24, 1993Date of Patent: November 28, 1995Assignee: Becton, Dickinson and CompanyInventors: George T. Walker, James G. Nadeau, Patricia A. Spears, Colleen M. Nycz, Daryl D. Shank, James L. Schram, Stewart R. Jurgensen
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Patent number: 5314801Abstract: The invention provides methods and nucleic acid probes for deleting, amplifying, and isolating sequences of Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium paratuberculosis.Type: GrantFiled: November 6, 1992Date of Patent: May 24, 1994Assignee: Becton, Dickinson and CompanyInventors: Colleen M. Nycz, James L. Schram, Daryl D. Shank, Glenn P. Vonk