Abstract: The present invention relates to isolated polypeptides having pectinase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: The instant application provides methods to improve the total starch yield and/or gluten yield from corn kernels in a wet milling process, the method comprising admixing corn kernels or a fraction of the corn kernels with an enzyme composition comprising an effective amount of one or more hydrolytic enzymes, wherein at least one of said hydrolytic enzymes is selected from the group consisting of a GH30 polypeptide, a GH5 polypeptide or a combination thereof.

Abstract: The instant application provides methods to improve the total starch yield and/or gluten yield from corn kernels in a wet milling process, the method comprising admixing corn kernels or a fraction of the corn kernels with an enzyme composition comprising an effective amount of one or more hydrolytic enzymes, wherein at least one of said hydrolytic enzymes is selected from the group consisting of a GH30 polypeptide, a GH5 polypeptide or a combination thereof.

Abstract: The present invention provides process for treating crop kernels, comprising the steps of a) soaking kernels in water to produce soaked kernels; b) grinding the soaked kernels; c) treating the soaked kernels in the presence of an effective amount of GH62 polypeptide having arabinofuranosidase activity or a GH43 polypeptide having arabinofuranosidase activity, wherein step c) is performed before, during or after step b).

Abstract: The present invention relates to processes for separating wheat flour into two or more fractions including a gluten fraction and a starch fraction, comprising the steps of: a) mixing wheat flour and water; b) adding one or more polypeptide (s) having GH8 xylanase activity; c) incubating the mixture for a predefined period of time; d) separating the mixture into two or more fractions including a gluten rich fraction and a starch rich fraction; and e) recovering the two or more fractions including a gluten rich fraction and a starch rich fraction.

Abstract: The present invention provides process for treating crop kernels, comprising the steps of a) soaking kernels in water to produce soaked kernels; b) grinding the soaked kernels; c) treating the soaked kernels in the presence of an effective amount of GH62 polypeptide having arabinofuranosidase activity or a GH43 polypeptide having arabinofuranosidase activity, wherein step c) is performed before, during or after step b).

Abstract: The present invention relates to a process for producing a high protein feed ingredient from a whole stillage byproduct produced in a starch-containing grain dry milling process for producing a fermentation product, as well as enzyme blends used in the processes for partitioning a greater amount of protein from the whole stillage byproduct into the high protein fraction, rather than being retained in the wet cake, to produce a high protein feed ingredient.

Abstract: Provided are improved methods for treating wheat flour with lipase. Further provided are methods for separating wheat flour to provide a gluten fraction, a starch fraction and a fibre fraction where the wheat flour is treated with a selected lipase. Polypeptides having lipase activity, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides are also provided.

Abstract: The present disclosure relates to processes for producing fermentation products from starch-containing material, wherein a triacylglyceride lipase (e.g., a thermostable triacylglyceride lipase) is present and/or added during liquefaction, pre-saccharification, saccharification, fermentation, simultaneous saccharification and fermentation, or any combination thereof, to increase enzymatically accessible starch, for example by reducing starch retrogradation, and/or increase fermentation product yield, such as ethanol yield. The disclosure also relates to the use of a triacylglyceride lipase in processes of the disclosure, for example, to increase enzymatically accessible starch and/or fermentation product yield, such as ethanol yield.

Abstract: The present invention provides process for treating crop kernels, comprising the steps of a) soaking kernels in water to produce soaked kernels; b) grinding the soaked kernels; c) treating the soaked kernels in the presence of an effective amount of GH62 polypeptide having arabinofuranosidase activity or a GH43 polypeptide having arabinofuranosidase activity, wherein step c) is performed before, during or after step b).

Abstract: A process for treating crop kernels is comprised of the steps of a) soaking kernels in water to produce soaked kernels; b) grinding the soaked kernels; c) treating the soaked kernels in the presence of an effective amount of GH62 polypeptide having arabinofuranosidase activity or a GH43 polypeptide having arabinofuranosidase activity, wherein step c) is performed before, during or after step b).

Abstract: Known georeferencing techniques require input in the form of manually-chosen anchor points or dense surveyed data. The present invention is an improved method and system for georeferencing underground geometric data. The method comprises (a) visiting at least two control points; (b) obtaining information about each of the at least two control points using scanning means; (c) recording the information about the at least two control points into a computer processor; and (d) performing a best-fit transformation to the recorded information. Preferably, the scanning means comprises laser scanners and at least two radio-frequency identification (RFID) tags. However, other technologies, such as retro-reflective LIDAR targets, Wi-Fi access points or bar codes and a bar code reader may also be used. In addition, sonar, radar, flash LIDAR, MEMS LIDAR, or any other similar technology could be used.

Abstract: The instant application provides methods to improve the total starch yield and/or gluten yield from corn kernels in a wet milling process, the method comprising admixing corn kernels or a fraction of the corn kernels with an enzyme composition comprising an effective amount of one or more hydrolytic enzymes, wherein at least one of said hydrolytic enzymes is selected from the group consisting of a GH30 polypeptide, a GH5 polypeptide or a combination thereof.

Abstract: The present invention relates to GH Family 11 xylanase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Abstract: The present invention relates to xylanase variants, comprising an alteration at least at one position corresponding to position 87 of the polypeptide of SEQ ID NO: 3, wherein the variant has xylanase activity and has increased xylanase inhibitor tolerance compared to the xylanase of SEQ ID NO: 3; and i) wherein the variant has at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide of SEQ ID NO: 3; or ii) wherein the number of alterations is 1-20, e.g., 1-10 such as 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 alterations. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Abstract: Provided are modified cellulase enzymes exhibiting increase cellulose-hydrolyzing activity in the presence of lignin and/or reduced binding to lignin comprising modified linker peptides comprising one or more amino acid substitutions, insertions, or deletions that result in (a) a decrease in the calculated isoelectric point of the linker peptide and/or (b) an increase in the ratio of threonine:serine in the linker peptide relative to a parental linker peptide from which said modified linker peptide is derived. Also provided are genetic constructs comprising nucleic acid sequences encoding for modified cellulase enzymes, methods for the production of the modified cellulase enzymes from host strains and a process for hydrolysing cellulose with the modified cellulases in the presence of lignin.

Abstract: A process for treating crop kernels is comprised of the steps of a) soaking kernels in water to produce soaked kernels; b) grinding the soaked kernels; c) treating the soaked kernels in the presence of an effective amount of GH62 polypeptide having arabinofuranosidase activity or a GH43 polypeptide having arabinofuranosidase activity, wherein step c) is performed before, during or after step b).

Abstract: Known georeferencing techniques require input in the form of manually-chosen anchor points or dense surveyed data. The present invention is an improved method and system for georeferencing underground geometric data. The method comprises (a) visiting at least two control points; (b) obtaining information about each of the at least two control points using scanning means; (c) recording the information about the at least two control points into a computer processor; and (d) performing a best-fit transformation to the recorded information. Preferably, the scanning means comprises laser scanners and at least two radio-frequency identification (RFID) tags. However, other technologies, such as retro-reflective LIDAR targets, Wi-Fi access points or bar codes and a bar code reader may also be used. In addition, sonar, radar, flash LIDAR, MEMS LIDAR, or any other similar technology could be used.

Abstract: The present invention provides process for treating crop kernels, comprising the steps of a) soaking kernels in water to produce soaked kernels; b) grinding the soaked kernels; c) treating the soaked kernels in the presence of an effective amount of an enzyme composition comprising: i) a protease, and ii) a cellulolytic composition, wherein step c) is performed before, during or after step b).

Abstract: The present invention relates to GH Family 11 xylanase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.