Abstract: Described herein, to overcome the limitations of conventional antibiotics, antibiotics have been encapsulated in biocompatible particles manufactured using Particle Replication In Non-wetting Templates (PRINT). These PRINT antibiotic particles were assessed as a topical agent to prevent E. coli infection using in vitro and in vivo models relevant to UTI and neurogenic bladder. The results show a prolonged efficacy and wide distribution in the bladder, resulting in a prophylactic environment in the bladder. The subject matter described herein is directed to molded particles containing an antibiotic active agent and methods of treating diseases and conditions with the particles, and methods of preparing the particles and compositions comprising the particles.