Abstract: An aminoacylation/translation (AIT) system based on the protein synthesis system from the pathogen Pseudomonas aeruginosa, was used to screen chemical compounds for identifying inhibitors of protein synthesis. This system includes elongation factors: EF-Tu, EF-Ts and EF-G, aminoacyl tRNA synthetase (aaRS) specific for phenylalanine, PheRS, and ribosomes isolated from cultures of Pseudomonas aeruginosa. Compounds identified using this assay have been shown to contain broad spectrum activity against both Gram+ and Gram? pathogens. Methods of using the identified compounds, as well as derivatives and analogues of these compounds, as antimicrobial agents against bacterial infections are described.

Abstract: An aminoacylation/translation (AIT) system based on the protein synthesis system from the pathogen Pseudomonas aeruginosa, was used to screen chemical compounds for identifying inhibitors of protein synthesis. This system includes elongation factors: EF-Tu, EF-Ts and EF-G, aminoacyl tRNA synthetase (aaRS) specific for phenylalanine, PheRS, and ribosomes isolated from cultures of Pseudomonas aeruginosa. Compounds identified using this assay have been shown to contain broad spectrum activity against both Gram+ and Gram? pathogens. Methods of using the identified compounds, as well as derivatives and analogues of these compounds, as antimicrobial agents against bacterial infections are described.

Abstract: Reconstituted bacterial replication assemblies and methods for their use are provided.

Abstract: Reconstituted bacterial replication assemblies and methods for their use are provided.

Abstract: Mutant bacterial MRS nucleic acids and proteins are provided, as well as uses for the nucleic acids and proteins, such as in a high-throughput screen for inhibitors of the mutant proteins.

Abstract: Y. pestis and P. aeruginosa nucleic acid molecules encoding dnaE, holA, holB, holC, holD, holE, dnaX, dnaN, SSB, dnaG, dnaQ, proteins are provided. The encoded proteins are also provided. The nucleic acid molecules and proteins are useful for reconstituting replicases and polymerases for sequencing, amplification, and screening for compounds which modulate the function of the polyemersase or replicase.

Abstract: The present invention relates to gene and amino acid sequences encoding DNA polymerase III holoenzyme subunits and structural genes from thermophilic organisms. In particular, the present invention provides DNA polymerase III holoenzyme subunits and accessory proteins of T. thermophilus. The present invention also provides antibodies, primers, probes, and other reagents useful to identify DNA polymerase III molecules.

Abstract: The present invention relates to gene and amino acid sequences encoding DNA polymerase III holoenzyme subunits and structural genes from thermophilic organisms. In particular, the present invention provides DNA polymerase III holoenzyme subunits and accessory proteins of T. thermophilus. The present invention also provides antibodies, primers, probes, and other reagents useful to identify DNA polymerase III molecules.

Abstract: Gene and amino acid sequences encoding DNA polymerase III holoenzyme &dgr; subunits and structural genes from bacteria are provided. Also provided are antibodies and other reagents useful to identify DNA polymerase III &dgr; subunit molecules. Also provided are methods to identify DNA polymerase III &dgr; subunit molecules. The use of DNA polymerase III &dgr; subunit molecules in assays to identify candidate antibiotics are also provided.

Abstract: A spinning shot gun projectile comprises a cylindrical body having radially projecting vanes arranged with respect to the longitudinal axis of the body at an angle predetermined for air impingement during the flight of the projectile, thereby imparting a spinning motion to the same and insuring an accurate trajectory. The rearward portion of the projectile body has an axial bore dimensioned to receive in bearing engagement the ram portion of wad means located forwardly of the powder. The thrust of the explosion thus is transmitted to the projectile centrally and forwardly of the same by the action of the ram, thereby further insuring true flight of the projectile.