Abstract: System for quantifying analyte polynucleotides employs computer-implemented analysis of real-time amplification data using a calibration curve defined by parametric equations.

Abstract: System for analyzing a polynucleotide-containing sample using real-time amplification and monitoring. The system includes a computer or processing device with software for performing a vector analysis of growth curves. The vector analysis advantageously simplifies polynucleotide quantitation by circumventing the need to establish thresholds used for calculating initiation of the growth phase, or to calculate derivatives.

Abstract: Nucleic acid oligomers specific for human parvovirus genomic DNA are disclosed. An assay for amplifying and detecting human parvovirus genotypes 1, 2 and 3 nucleic acid in biological specimens is disclosed. Compositions for amplifying and detecting the presence of human parvovirus genotypes 1, 2 and 3 genomic DNA in human biological specimens are disclosed.

Abstract: Method for quantifying analyte polynucleotide in a test sample using real-time amplification and adjustment of a stored calibration curve. The method may be practiced using as few as a single adjustment calibrator to adjust the stored curve. This simplifies the quantitative analysis procedure, while still providing the advantages of internal calibration adjustment to account for variation in amplification reaction efficiency.

Abstract: Methods and kits for preparing and adjusting pre-defined master calibration curves of the type used for quantifying analyte polynucleotides by real-time nucleic acid amplification. Particularly disclosed are methods of preparing one or more master calibration curves on one instrument, and then using those master calibration curves on a different instrument.

Abstract: Nucleic acid assays for detecting nucleic acids of Dengue virus serotypes 1-4.

Abstract: System for quantifying analyte polynucleotides employs computer-implemented analysis of real-time amplification data using a calibration curve defined by parametric equations.

Abstract: System for analyzing a polynucleotide-containing sample using real-time amplification and monitoring. The system includes a computer or processing device with software for performing a vector analysis of growth curves. The vector analysis advantageously simplifies polynucleotide quantitation by circumventing the need to establish thresholds used for calculating initiation of the growth phase, or to calculate derivatives.

Abstract: Method of preparing a calibration curve and determining the amount of an analyte polynucleotide present in a test sample, particularly using real-time amplification data. The method involves determining indicia of amplification for both the internal calibrator and analyte polynucleotide standards, each as a function of the amount of analyte polynucleotide standard input into the amplification reaction.

Abstract: Machine executable method of analyzing growth curve data to identify the transition from a baseline phase into a growth phase. Applications of the method include analysis of results from time-dependent monitoring of amplicon synthesis in a nucleic acid amplification reaction to quantify a starting amount of a nucleic acid template in a test sample. The method advantageously simplifies the quantitation by circumventing the need to establish thresholds used for calculating initiation of the growth phase, to calculate derivatives, or to perform linear regression analysis.

Abstract: Compositions, methods and kits for detecting Chikungunya viral nucleic acids. Particularly described are methods for detecting very low levels of the viral nucleic acids using nucleic acid amplification.

Abstract: Methods and kits for preparing and adjusting pre-defined master calibration curves of the type used for quantifying analyte polynucleotides by real-time nucleic acid amplification. Particularly disclosed are methods of preparing one or more master calibration curves on one instrument, and then using those master calibration curves on a different instrument.