Abstract: In a search engine server, a method for searching for data in a data network comprising hyperlinked pages comprising the steps of (1) receiving an initial set of network addresses for pages in the data network; (2) receiving a non-negative integer, N, specifying a chain length; (3) receiving a set of at least one search argument comprising search criteria; and (4) performing a search wherein all pages linked to said initial set of addresses by a chain of distance less than or equal to N are examined for compliance with the search criteria, and all pages meeting such criteria are returned as successful objects of the search. According to optional embodiments, the foregoing method can be implemented as a computer readable medium with instructions for performing the above steps, as an application program, or a browser resident at an end user's computer system. It is also possible to implement as a special purpose information handling system.

Abstract: A method for searching for data in a data network comprising hyperlinked pages comprising the steps of (1) receiving an initial set of network addresses for pages in the data network; (2) receiving a non-negative integer, N, specifying a chain length; (3) receiving a set of at least one search argument comprising search criteria; and (4) performing a search wherein all pages linked to said initial set of addresses by a chain of distance less than or equal to N are examined for compliance with the search criteria, and all pages meeting such criteria are returned as successful objects of the search. According to optional embodiments, the foregoing method can be implemented as a computer readable medium with instructions for performing the above steps, as an application program, or a browser resident at an end user's computer system. It is also possible to implement as a special purpose information handling system.

Abstract: A method whereby the blood pressure metabolism in an individual showing evidence of dysregulation is improved when that person receives an appropriate oral administration of (−)-hydroxycitric acid. The potassium salt of (−)-hydroxycitric acid is a preferred form of the compound, followed by the sodium salt, then by the amide and other derivatives of the acid. The regulation of blood pressure levels over any given period of time may be improved with a controlled release form of (−)-hydroxycitric acid. Controlled release can be used to provide a sustained and modulated amount of the active to the body as desired and therefore regulate the use of the compound as a hypotensive agent.

Abstract: Sequencing of a selected region of a target nucleic acid polymer in a natural abundance DNA sample can be performed in a single vessel by combining the sample with a sequencing mixture containing a primer pair, a thermally stable polymerase such as Thermo Sequenase™ which incorporates dideoxynucleotides into an extending nucleic acid polymer at a rate which is no less than about 0.4 times the rate of incorporation of deoxynucleotides, nucleotide feedstocks, and a chain terminating nucleotide. The reaction mixture also includes an unconventional nucleotide and an appropriate enzyme for degradation of nucleic acid polymers containing the unconventional nucleotide. The mixture is processed through multiple thermal cycles for annealing, extension and denaturation to produce a product mixture which is analyzed by electrophoresis.

Abstract: A hierarchy of at least two assay techniques is utilized in testing for disease-associated mutations. The first assay in the hierarchy is selected to provide a highly specific test for the existence of the disease-associated mutation, although the accuracy of the test need not be high. The final assay in the hierarchy is selected to provide a highly accurate and highly specific test for the existence of the disease associated mutation. Intermediate tests of progressively greater accuracy may also be included in the hierarchy. Once the hierarchy has been selected for a given mutation-associated disease, a patient sample is analyzed the patient sample using the first, lowest accuracy assay in the hierarchy. If the result of the first assay is negative for the presence of a disease-associated mutation, then the next assay in the hierarchy is performed.

Abstract: A method for obtaining information about the allelic type of a sample of genetic material derived from an HIV-infected sample relies on the observation that using a second nested set of sequencing primers ensures the maximum potential for sequencing a particular sample. To perform the method, reagents suitable for performing the tests are suitably packaged as a kit.

Abstract: Direct determination of the sequence of an RNA sample is performed under isothermal conditions. An RNA sample containing a target nucleic acid is combined in a single reaction vessel with a reaction mixture containing two polynucleotide primers, a first primer that specifically hybridizes with a target sequence near the 3′ end of the target nucleic acid, and a second primer that specifically hybridizes to the 3′ end of an antisense copy of the target nucleic acid. At least one of the primers is labeled with a detectable label, and at least one of the first or second primer has an RNA polymerase transcription initiation signal at its 5′ end, which signal does not specifically hybridize to the RNA target.

Abstract: Evaluation of a sample for the presence and qualitative nature of a microorganism can be performed in a single vessel by combining a natural abundance DNA sample with a sequencing mixture containing a primer pair, a thermally stable polymerase such as ThermoSequenase™ which incorporates dideoxynucleotides into an extending nucleic acid polymer at a rate which is no less than about 0.4 times the rate of incorporation of deoxynucleotides, nucleotide triphosphate feedstocks, and a chain terminating nucleotide triphosphate. The mixture is processed through multiple thermal cycles for annealing, extension and denaturation to produce a product mixture which is analyzed by electrophoresis.

Abstract: A method whereby the glucose metabolism in an individual showing evidence of dysregulation, as is found in insulin resistance, reactive hyperglycemia and/or elevated blood sugar levels and/or diabetes, is improved when that person receives an appropriate oral administration of (−)-hydroxycitric acid. The potassium salt of (−)-hydroxycitric acid is the preferred form of the compound, followed by the sodium salt. The regulation of glucose levels over any given period of time may be improved with a controlled release form of (−)-hydroxycitric acid. Controlled release can be used to provide a sustained and modulated amount of the active to the body as desired and therefore regulate the use of the compound as a hypoglycemic agent.

Abstract: A system and method to implement callback for unanswered telephone calls in an Internet Telephony Callback system. A calling station and a called station are each connected to the system through a local and remote service provider, respectively, each provider including a voice gateway and callback server. A telephone call originated at the calling station by a calling party is processed by the local service provider and the local callback server generates a control message which accompanies the telephone call. The remote service provider processes and connects the telephone call if answered by the called party and sends a message to delete the control message from the local callback server. If the telephone call is unanswered the remote callback server stores the control message and waits for the called party to initiate a return call. When the return call is initiated, the remote callback server sends a message to the local callback server to extend the return call to the calling party.

Abstract: Secure communication may be conducted between two or more parties over a network, e.g the Internet without prior security arrangements among the parties or agreed to encryption/decryption software. A sending party is connected to a data network through a computer and has access to a communications network, e.g. a public switched telephone network. The sender prepares a file designated, e.g. “X” containing confidential information for secure transmission over the Internet or the like to one or more receivers. In one embodiment, the sender downloads encryption/decryption or “crypto” software stored at a location on the Internet e.g. location “U” in a Uniform Resource Locator (URL). The “crypto” software is written in executable code or an interpretive language such as JAVA. The sender selects a key “K” and encrypts the plain text file “X” into cipher text.

Abstract: A presently disclosed radio broadcasting system for a virtual radio program broadcasting station uses a divided regional approach to broadcast digital and analog signals over a large geographic region divided into multiple overlapping but separate areas constituting small portions of the region. The small areas are served by separate transmission sources/towers supplied from a common source central to the station. The system supports reuse of allocated transmission parameters within non-neighboring small areas in the region. The station is "virtual" because its central source need not be in any of the small areas, and because it uses different transmission parameters in neighboring small areas in a manner that previously would be used by plural different stations. System transmissions include information signals sent in both analog and digital forms.

Abstract: A communication system includes a client controlled gateway enabling concurrent voice/data transmission in a distributed information network and a communication network between the client and a service representative of a host system provider. A computer and a modem couple the client to the gateway in the communication network over a single communication path. The computer includes a browser for searching the distributed network. The browser includes "plug-in" programs which interact with the data server in supporting voice calls between the client and the host system. The gateway functions as a shared voice data (SVD) server to couple the client to the data server after authentication of the client's identification (ID) and password. The gateway transfers data between the client and the data server over the distributed network in accordance with screens presented to the client by the host system.

Abstract: A user through a personal computer, in real time, controls the activation and selection of voice and data transmission paths in a communication network independent of network profiles. The network includes a public switched telephone network and a data network, e.g. the Internet for voice and/or data transmissions over either the voice or data networks. In the network, a voice terminal and a computer terminal are coupled to a shared voice and data terminal. A digital subscriber line access multiplexer is coupled to the shared voice and data terminal; the data network through a client server interface, and a voice network through an intelligent switch. The client server interface includes programmable stored data descriptive of network-authorized data and voice functions and transmission paths in the voice and data networks. The interface is linked to the multiplexer and switch for transmitting signaling messages directing traffic to the voice and data networks in accordance with the stored data.

Abstract: A method is provided for simultaneously determining the positions of a selected nucleotide base in a target region of both strands of a denatured duplex nucleic acid polymer. The nucleic acid polymer is combined with a reactant mixture comprising first and second oligonucleotide primers, said primers binding to the sense and antisense strands, respectively, of the nucleic acid polymer at a location flanking the target region; a thermostable DNA polymerase; a chain-terminating nucleotide triphosphate complementary to the selected nucleotide base; and other reagents for synthesis of chain extension products to form a reaction mixture. This mixture is processed through a plurality of thermal cycles, each including at least a chain extension phase and a denaturation phase to produce chain extension products. These chain extension products are evaluated to determine the positions of the selected bases.

Abstract: Rapid and cost effective diagnosis of p53 mutations of a sample of patients is achieved by employing a selected plurality of diagnostic tools, in a hierarchy of increasing accuracy and cost per tool, in which each tool detects essentially no false positives. Diagnostic tests that may be included among the plurality of tests selected include, in order of increasing accuracy and cost:(a) immunoassays,(b) analysis of DNA from a patient sample by quantitative amplification of p53 exons using amplification primers complementary to intron regions flanking each exon and examination of the length or quantity of each amplified fragment for nucleotide insertions or deletions relative to the normal p53 gene.

Abstract: A record playback system includes a display showing elapsed time of a record playback operation together with symbols indicating occurrences of certain sequences of sound during the playback operation, the symbols positioned to indicate times at which respective sequences of sounds occur. In a preferred application, the records reproduced in the system are audible voice-mail messages, the specific sequences of sounds are numbers or sets of numbers spoken consecutively during the message, and the symbols representing such numbers are printed characters corresponding to respective numbers. In the preferred application, the messages are centrally recorded at a server of a computer network and distributed to individual client computers via the network.

Abstract: Reliable and cost effective testing for mutations in the RB1 gene can be accomplished by (a) quantitatively amplifying exons of the sample RB1 gene using primers complementary to intron regions flanking each exon; and (b) determining the lengths and/or quantities of the amplification products for each exon and comparing that length or quantity to the length or quantity of amplification products obtained when a wild-type RB1 gene is amplified using the same primers. Differences in length between an amplified sample exon and the corresponding amplified wild-type exon reflect the occurrence on an insertion or deletion mutation in the sample RB1 gene. Differences in quantity reflect the complete absence of an exon, or heterozygosity for a mutant exon. Next, the nucleic acid sequence of each exon found to contain an insertion or deletion mutation is determined, or of all exons in the event no insertion or deletion mutations are identified.

Abstract: A newly defined facsimile (fax) handling service, to be performed by carriers in the public switched telephone network (PSTN) on calls originating from standard analog fax sending equipment, includes: a) intercepting and recording direct dialed digits, representing final destinations of such calls, at PSTN switching nodes in areas local to respective call origins; b) returning acknowledgements from these nodes to respective callers in the form of fax tones corresponding to those usually returned by standard analog fax receiving equipment; c) locally intercepting and recording signals representing the fax image information intended for delivery to the called destinations; d) returning a completion signal indication to the originating equipment; and e) offline to the originating equipment, managing the delivery of the recorded image information to the final destinations represented by the recorded call digits.

Abstract: A ligase-based assay which relies only upon knowledge of the wild-type sequence of a gene or gene fragment is used to detect all types of mutations, i.e., point mutations, insertions and deletions. The assay makes use of a set of oligonucleotide probes, which may be packaged in kit form, which hybridize in series along the length of the gene. The ligation of the probes together form a ligation product, the size of which is evaluated. When the gene or gene fragment being analyzed corresponds to the normal sequence and thus perfectly matches the probes, all of the probes in the set are ligated together, and the ligation produce has a certain resulting size. When a mutation appears in the gene, the hybridization of the probe overlapping the mutation is impaired, with the result that some or all of the ligation produce is of smaller size. By evaluating the size of the ligation product, both the existence of a mutation and its approx. position can be identified.