Abstract: A composition of matter comprises one or more cell lines configured to inducibly differentiate to at least a first stage of differentiation and a second, subsequent stage of differentiation. Each of the one or more cell lines are genetically edited to express one or more first stage inserted secretable reporter genes placed under control of promoters for genes canonically expressed during the first stage of differentiation. The cell lines are further genetically edited to express one or more second stage inserted secretable reporter genes placed under control of promoters for genes canonically expressed during the second stage of differentiation, but not during the first stage of differentiation, wherein the one or more second stage inserted secretable reporter genes are different than the one or more first stage inserted secretable reporter genes.

Abstract: A method for generating a reporter cell line comprises culturing a cell line capable of undergoing two or more consecutive stages of differentiation and performing the targeted insertion of two or more secretable reporter genes into the genome of the cultured cell line to form edited cells. One or more first stage inserted secretable reporter genes are placed under control of promoters for genes canonically expressed during the first stage of differentiation, and one or more second stage inserted secretable reporter genes are placed under control of promoters for genes canonically expressed during the second stage of differentiation but not during the first stage of differentiation. Differentiation of the clonally expanded edited cells is then induced to the first stage of differentiation, thus inducing expression of the first stage inserted secretable reporter genes.

Abstract: One aspect of this disclosure relates to a composition of matter. The composition of matter comprises a nucleotide construct encoding a peptide. The peptide includes at least a targeting domain configured to bind to chromatin having a pattern of reduced epigenetic repression, and a DNA strand break inducing domain. When accumulated through binding at chromatin sites, the strand break inducing domain may cause specific, double-strand breaks to the DNA, inducing cell death in cells exhibiting the pattern of reduced epigenetic repression.

Abstract: Mechanisms are provided in which a previously verified SoC is coupled to a SoC under test via a communication bus or other type of communication interface. The previously verified SoC is provided with the same test stimuli as the SoC under test and thus, generates expected test results data. The test stimuli are sent to the SoC under test via a peripheral communication interface between the previously verified SoC and the SoC under test. The SoC under test generates actual test results data that is output to the previously verified SoC. The previously verified SoC may then compare the expected test results data with the actual test result data generated by the SoC under test to determine if they match. If the two sets of data do not match, then a mismatch notification may be generated and output.

Abstract: An apparatus and method are provided in which a previously verified SoC is coupled to a SoC under test via a communication bus or other type of communication interface. The previously verified SoC is provided with the same test stimuli as the SoC under test and thus, generates expected test results data. The test stimuli are sent to the SoC under test via a peripheral communication interface between the previously verified SoC and the SOC under test. The SoC under test generates actual test result data that is output to the previously verified SoC. The previously verified SoC may then compare the expected test results data with the actual test result data generated by the SoC under test to determine if they match. If the two sets of data do not match, then a mismatch notification may be generated and output.

Abstract: A method is provided in which a previously verified SoC is coupled to a SoC under test via a communication bus or other type of communication interface. The previously verified SoC is provided with the same test stimuli as the SoC under test and thus, generates expected test results data. The test stimuli are sent to the SoC under test via a peripheral communication interface between the previously verified SoC and the SoC under test. The SoC under test generates actual test result data that is output to the previously verified SoC. The previously verified SoC may then compare the expected test results data with the actual test result data generated by the SoC under test to determine if they match. If the two sets of data do not match, then a mismatch notification may be generated and output.

Abstract: Methods, systems and computer program products are provided which update firmware in a network computer by replacing the standard operating system to be loaded at the initialization of the network computer with a firmware update operating system. The firmware update operating system is then downloaded to the network computer and initiated to update the firmware of the network computer. The firmware update operating system may then be replaced with the standard operating system to be loaded at the initialization of the network computer. The network computer may then be reinitialized by, for example, a cold boot, so as to load the standard operating system. The cold boot may be server initiated so as to allow for firmware updates with intervention by an operator at the network computer.

Abstract: Simultaneous use of multiple expanded memory cards and their associated Expanded Memory Manager (EMM) device drivers in a computer system is provided. First, an expanded memory card is added and its EMM device driver will check to verify if another EMM driver is currently installed. If so, the new EMM device driver will take control of the existing EMM device driver in a master-slave relationship. The new EMM device driver (master) controls the existing EMM device driver (slave) by use of the slave's interrupt vector. That is, the master controls the slave by having an interrupt vector for the slave readdressed to be input to the master. Thereby, providing the master with the which expanded memory interrupts the slave was required to service. The master also takes control of all the slave's handles and pages by appearing as a requesting program application to the slave.

Abstract: A system and method makes use of the line-in, line-out connectors for each right and left stereo channel of an audio card to provide a communications network. The distributed computers are connected in a master/slave configuration. All of the slave systems have the line-in ports, and the line-out ports, connected together. The master system line-out is connected to each slave system line-in port, and the slave system line-out ports are connected to the master line-in port, for each channel. A communications protocol is provided wherein the master system allows audio and data information to be simultaneously transferred between the master and slave systems. The master provides a clock signal and performs arbitration in order to facilitate the information transfer.

Abstract: Simultaneous use of multiple expanded memory cards and their associated Expanded Memory Manager (EMM) device drivers in a computer system is provided. First, an expanded memory card is added and its EMM device driver will check to verify if another EMM driver is currently installed. If so, the new EMM device driver will take control of the existing EMM device driver in a master-slave relationship. The new EMM device driver (master) controls the existing EMM device driver (slave) by use of the slave's interrupt vector. That is, the master controls the slave by having an interrupt vector for the slave readdressed to be input to the master. Thereby, providing the master with the expanded memory interrupts the slave was required to service. The master also takes control of all the slave's handles and pages by appearing as a requesting program application to the slave.

Abstract: An EMM device driver command installation line is provided which may be easily and quickly interpreted by a user, an installation or a setup program. The physical memory is correlated to the installation line by a map of the 16 K-byte DOS memory segments from 0 to the memory segment above 1024 K-bytes. Different characters are assigned to represent the different types of parameters which may be found in DOS memory (0 to 1024 K-bytes), e.g. read only memory (ROM), or video memory. Additionally, characters may represent the page (single 16 K-byte segment and frame (4 sequential pages) locations that are usable by the EMM device driver, and informative to a user. In addition to the type of characters used, the character positions also provide usable information to the user since a one to one correspondence exists between the character position and the physical locations in DOS memory.

Abstract: A method and apparatus utilizing one or two cameras are described for visually inspecting a polygonal component located at the end of the robotic end effector for determining presence, position and orientation of component leads prior to placement. Image processing improvements are provided for decreasing computational complexity by representing two dimensional image areas of interest, and each containing leads along one side of a component, by one dimensional summation profiles.

Abstract: A method is disclosed for determining the presence and position of fiducials on the surface of a printed circuit substrate in grey level images to sub-pixel accuracy. A summation profile technique is utilized to decrease the amount of computation required and thereby increase throughput for enabling real time operation in a total component placement system.

Abstract: In a printing system comprising a printer controlled by a host processor (11), a system of justification is provided wherein the determination of justification parameters is shared by the host processor (11) and the printer formatting processor (12). Justification means (25) in the host processor (11) determine which spaces in a line are to be altered to effect justification. Means (26, 28 and 13) are provided for conveying data from the host processor to the printer indicating which spaces in a particular line are to be so altered, and means (42) are provided in the printer for determining the size of the indicated spaces necessary to effect such a justification.