Abstract: A fully integrated and disposable point-of-care device for detecting a target nucleic acid is provided. The device comprises: an extraction chamber adapted to receive a biological sample, wherein said extraction chamber comprises means to extract and lyse the sample to release nucleic acid; a first amplification chamber in communication with the extraction chamber, wherein said amplification chamber comprises means to trigger nucleic acid amplification of a target nucleic acid sequence to occur; and a detection chamber in communication with the amplification chamber, wherein said detection chamber comprises means to detectably label the target nucleic acid and means to detect a signal associated with labeled target nucleic acid, or a single chamber for amplification, detection and identification of multiple nucleic acid sequences.

Abstract: A fully integrated and disposable point-of-care device for detecting a target nucleic acid is provided. The device comprises: an extraction chamber adapted to receive a biological sample, wherein said extraction chamber comprises means to extract and lyse the sample to release nucleic acid; a first amplification chamber in communication with the extraction chamber, wherein said amplification chamber comprises means to trigger nucleic acid amplification of a target nucleic acid sequence to occur; and a detection chamber in communication with the amplification chamber, wherein said detection chamber comprises means to detectably label the target nucleic acid and means to detect a signal associated with labeled target nucleic acid, or a single chamber for amplification, detection and identification of multiple nucleic acid sequences.

Abstract: A point-of-care device for detecting a target nucleic acid is provided. The device comprises: an extraction chamber adapted to receive a biological sample, wherein said extraction chamber comprises means to extract and lyse the sample to release nucleic acid; a first amplification chamber in communication with the extraction chamber, wherein said amplification chamber comprises means to trigger nucleic acid amplification of a target nucleic acid sequence to occur; and a detection chamber in communication with the amplification chamber, wherein said detection chamber comprises means to detectably label the target nucleic acid and means to detect a signal associated with labeled target nucleic acid.

Abstract: Electronically controlled film transport methods and systems are described. One system described comprises a film transport path for transporting film, an input drive assembly for advancing film through the film transport path, an output drive assembly for advancing film out of the film transport path, an aperture positioned in the film transport path, at least one registration pin capable of engaging at least one perforation in the film to secure a portion of the film in the aperture, wherein the at least one registration pin is capable of retracting to a non-engaging position, and a controller for controlling the retraction of the at least one registration pin.

Abstract: Electronically controlled film transport methods and systems are described. One system described comprises a film transport path for transporting film, an input drive assembly for advancing film through the film transport path, an output drive assembly for advancing film out of the film transport path, an aperture positioned in the film transport path, at least one registration pin capable of engaging at least one perforation in the film to secure a portion of the film in the aperture, wherein the at least one registration pin is capable of retracting to a non-engaging position, and a controller for controlling the retraction of the at least one registration pin.

Abstract: The present invention is directed to a method of determining the genotype of a human papilomavirus in a sample by amplifying a portion of the L1 open reading frame of human papilomavirus genome with the amplification primer having SEQ ID NO:2, the sequencing primer having SEQ ID NO:3 and an additional sequencing primer specific to HPV51 having SEQ ID NO:5.