Abstract: The present disclosure relates generally to the field of immunology, and particularly relates to compositions, methods, and systems for the analysis and generation of antigen-binding molecules produced by immune cells obtained from tissue samples (e.g., antibodies produced by B cells in tumor tissue samples or TCRs produced by T cells in tumor tissue samples) using spatial methodologies, and for the production and characterization of recombinant antigen-binding molecules (e.g., antibodies, TCRs) with desired properties.

Abstract: Described herein are methods and related systems and computer program products for assessing the antigen binding specificity of an antigen binding molecule (ABM) expressed by one or more immune cells and may include contacting a plurality of cells comprising at least one antigen binding molecule with a first target antigen and a control; determining a first readout based on the binding of the at least one antigen binding molecule to the first target antigen; determining a second readout based on the binding of the at least one antigen binding molecule to the control; and determining, from the first readout and the second readout, a specificity metric representing the specificity of the at least one antigen binding molecule binding to the target antigen relative to the control.

Abstract: The present disclosure relates in some aspects to methods and compositions for assessing the target binding capacity of a binding molecule, such as an antigen binding molecule, e.g., a monoclonal antibody. In some embodiments, provided herein is a method of analyzing binding of a barcode-labeled antibody to a target antigen expressed by a cell, wherein cells expressing the candidate antigens are partitioned and wherein detection of the barcoded nucleic acid molecule is indicative of the binding between the antibody or antigen-binding fragment thereof and the candidate antigen or epitope of the cell.

Abstract: The present disclosure relates generally to compositions, methods, and systems for the characterization of binding characteristics of antigen binding molecules, e.g., antibodies, using single-cell immune profiling methodologies. The compositions, methods and systems described herein permit rapid, high-throughput identification and characterization of antigen binding molecules having desired properties.

Abstract: The present disclosure relates generally to compositions, methods, and systems for the characterization of antigen-binding molecules, e.g., multispecific T cell receptors, B cell receptors, antibodies, and antigen-binding fragments thereof, using single-cell immune profiling methodologies. The compositions, methods and systems described herein permit rapid, high-throughput identification and characterization of multispecific antigen binding molecules having desired properties.

Abstract: The present disclosure generally relates to methods and systems for engineering antibodies, and antigen-binding fragments thereof, to have altered characteristics. The present disclosure also provides a high-throughput method useful for identifying the engineered antibodies, or antigen-binding fragments thereof, that acquired the altered characteristics having performed the methods. These methods and systems have implications, for example, in the rapid development of biotherapeutics having desired and/or improved, e.g., affinity, specificity and/or activity-related, characteristics.

Abstract: A method is provided for identifying one or more D gene segment in a VDJ or VDDJ sequence. The method can include obtaining a B cell receptor and/or T cell receptor data set, wherein the data set includes a VDJ sequence, aligning the VDJ sequence to one or more VDJ reference sequences thereby generating a first potential alignment and a second potential alignment, determining a first score for the first potential alignment and a second score for the second potential alignment in accordance with a D gene segment alignment scoring schema, and identifying a D gene segment region associated with a highest score between the first score and the second score.

Abstract: A device and method are provided for determining whether a laminate is bonded or debonded from its substrate. The device and method provide simple, accurate, rapid, cost-effective, and reliable means for assessing the bonding state of a laminated substrate.

Abstract: Interactive visualization systems and methods are disclosed herein. The system includes a data source, user input device, processor, and display. The data source obtains a data set comprising B cell receptor and/or T cell receptor data associated with a plurality of cells. The user input device receives a user-selected first parameter under which to analyze the data set. The processor performs the method by identifying a plurality of clonotype groups in the data set using the first parameter, identifying subclonotypes within the clonotype groups (wherein each identified subclonotype comprises cells having identical V(D)J transcripts), and processing the data to define a visualization model that can display a compressed view of the clonotype groups and of the plurality of subclonotypes. The display renders a visualization of said data set according to said visualization model.

Abstract: The disclosed subject matter describes herein a method for on-boarding virtualized network function (VNF) packages in a network functions virtualization (NFV) system that includes receiving a VNF package archive (VPAR) file containing a VNF package and accessing a VNF descriptor (VNFD) file in a VNFD directory contained within the VPAR file. The method further includes uploading the VNFD file from the VNFD directory to a VNF catalog in the NFV system and deploying, in a virtual environment associated with the NFV system, one or more VNFs in accordance to the contents of the uploaded VNFD file and an associated VNF image file.

Abstract: According to one example, the disclosed subject matter describes herein a method for on-boarding VNF packages in a network functions virtualization (NFV) system that includes receiving a virtualized network function (VNF) package archive (VPAR) file containing a VNF package and accessing a VNFD file in a VNFD directory contained within the VPAR file.

Abstract: In one embodiment, a central service request management system for managing service requests across a plurality of ticketing systems. The central service request management system includes translation logic for receiving service request messages from the plurality of ticketing systems and translating the service request messages to a standardized format, where each service request message is associated with a respective service request. Additionally, the central service request management system includes data storage logic for maintaining a status of service requests based on the translated service request messages. The central service request management system further includes routing logic for identifying one of the plurality of ticketing systems to transmit each translated service request message to based on the maintained status of the service request associated with the service request message, and for transmitting the translated service request message to the identified ticketing system.

Abstract: The present disclosure provides a concealed-carry firearm holster for wearing under the clothes of a user between the user's waist and a waistband of the user's pants. The holster generally includes a backing panel and a firearm receptacle connected to the backing panel and structured and operable to removably retain a firearm. The holster additionally includes pair of belt clips removably connected, via removable fasteners, to the backing panel and structured and operable to removably secure the holster to at least one of the waistband and belt threaded through belt loops of the user's pants to retain the holster in position under the user's clothes. Furthermore, the holster includes a pair of belt clip covers disposable over the belt clips, wherein the clip covers are structured and operable to resemble the belt loops of the user's pants.

Abstract: A firearm holster for storing a firearm within a vehicle such that the firearm is secure and easily accessible. The holster includes a backing panel and a firearm housing connected to the backing panel and structured and operable to provide a firearm receptacle to removably retain a firearm. The holster additionally includes a support panel connectable to the backing panel. The support panel structured and operable to be positioned and secured, along with the backing panel and firearm receptacle, between two closely adjacent structures of the vehicle and to contact a floor of the vehicle along a bottom edge such that the firearm receptacle is positionable at a desired height above the floor between the closely adjacent structures. The holster further includes a cinching strap connected to the backing panel. The cinching strap structured and operable to secure the holster to an anchor point within the vehicle.

Abstract: A method is provided in one example and includes verifying a storage capacity of a network element coupled to an end device over a network connection. The method also includes executing script provided in the network element, which is configured for exchanging packets in a network environment. The script initiates a collection of data being retrieved from the end device. The data can be Fault, Configuration, Accounting, Performance, and Security (FCAPS) data associated with the end device. The data collected from the end device is communicated to a next destination.

Abstract: A method is provided in one example and includes verifying a storage capacity of a network element coupled to an end device over a network connection. The method also includes executing script provided in the network element, which is configured for exchanging packets in a network environment. The script initiates a collection of data being retrieved from the end device. The data can be Fault, Configuration, Accounting, Performance, and Security (FCAPS) data associated with the end device. The data collected from the end device is communicated to a next destination.

Abstract: A method is provided in one example and includes verifying a storage capacity of a network element coupled to an end device over a network connection. The method also includes executing script provided in the network element, which is configured for exchanging packets in a network environment. The script initiates a collection of data being retrieved from the end device. The data can be Fault, Configuration, Accounting, Performance, and Security (FCAPS) data associated with the end device. The data collected from the end device is communicated to a next destination.

Abstract: A system, method and application for facilitating network support for an install-base network is provided. The method includes performing, at an access node of the install-base network, network discovery to discover one or more nodes of the install-base network. The method also includes collecting, at the nodes, their respective inventories (“network-node inventories”). The method further includes collecting the network-node inventories at the access node, and sending the network-node inventories from the access node to a back-office system external to the install-base network. The method may, optionally, include the access node aggregating the network-node inventories to form aggregate information, and sending the aggregate information to the back-office system in addition to or in lieu of the of network-node inventories.

Abstract: A physical object comprises one or more memory circuits adapted to be read wirelessly by a reader device attached to or incorporated within the physical object. Data in the memory circuit is protected from access by an unauthorised reader. Data in the memory circuit is also adapted to identify an authorised bearer of the physical object. Moreover, data in the memory circuit is adapted to allow access to a specified asset or assets by the authorised bearer.

Abstract: Apparatus for providing electronic data associated with an item to a user, the apparatus comprising a memory tag scanner arranged to communicate with a memory tag associated with an item, a user profile memory arranged to store user profile data therein, an output interface arranged to provide data to a user, and a data processor arranged to cause data to be read from the memory tag associated with an item and the data to be selectively provided to a user via the output interface in accordance with the stored user profile data.