Abstract: Provided herein are vectors comprising a polycistronic expression casstte comprising a polynucleotide that encodes a CD 19 specific chimeric antigen receptor, a polynucleotide that encodes a cytokine, and a polynucleotide that encodes a marker protein, wherein the polynucleotide that encodes the CD 19-specific chimeric antigen receptor and the polynucleotide that encodes the cytokine coding sequence are separated by a polynucleotide sequence that comprises an F2A element, and wherein the polynucleotide sequence that encodes the cytokine and the polynucleotide sequence that encodes the marker protein are separated by a polynucleotide sequence that comprises a T2A element.

Abstract: Provided herein are vectors comprising a polycistronic expression casstte comprising a polynucleotide that encodes a TCR alpha chain, a polynucleotide that encodes a TCR beta chain, and a polynucleotide that encodes a cytokine, wherein the polynucleotides are separated by polynucleotide sequences that comprise 2A elements.

Abstract: The present invention provides methods of treating glioblastoma by administering an adenoviral vector that conditionally expresses IL-12 via gene switch under control of an oral activator ligand.

Abstract: A method for fabricating a gas turbine engine combustor that includes a domeplate and at least one fuel injector extending through an opening in the domeplate. The method includes fabricating a heatshield that includes a threaded collar extending upstream from the heatshield, positioning the heatshield on a downstream side of the domeplate such that the threaded collar is received within the domeplate opening, and coupling a retainer to the collar on an upstream side of the domeplate such that the domeplate is securely coupled between the heat shield and the retainer.

Abstract: A gas turbine engine fuel nozzle includes an axis of symmetry extending therethrough, the nozzle body including a first passage extending coaxially therethrough, a second passage, and a third passage, the second passage circumscribing the first passage, the third passage formed radially outward of the second passage, and a nozzle tip coupled to the nozzle body, the nozzle tip including at least one primary discharge opening in flow communication with the first passage, at least one secondary discharge opening in flow communication with the second passage, and at least one tertiary discharge opening in flow communication with the third passage.

Abstract: A method enables a combustor for a gas turbine engine to be assembled. The method includes coupling an inner liner to an outer liner such that a combustion chamber is defined therebetween, wherein the outer liner is fabricated from a plurality of panels coupled together, and coupling an outer support radially outward from the outer liner such that an outer passageway is defined between the outer liner and the outer support, wherein the outer support is configured to channel cooling air from the outer passageway towards at least a portion of the outer liner.

Abstract: A combustor dome assembly for a gas turbine engine having a longitudinal centerline axis extending therethrough, including: an annular dome plate having an inner portion, an outer portion, a forward surface, and a plurality of circumferentially spaced openings formed therein, wherein a radial section defined between each of the openings includes a cooling trough formed therein; an outer cowl connected to the dome plate outer portion at a downstream end thereof; an inner cowl connected to the dome plate inner portion at a downstream end thereof; and, a deflector plate connected to and positioned aft of each opening in the dome plate.

Abstract: A combustor dome assembly for a gas turbine engine having a longitudinal centerline axis extending therethrough, including: an annular dome plate having an inner portion, an outer portion, a forward surface and a plurality of circumferentially spaced openings formed therein, wherein a radial section is defined between adjacent openings; an annular outer cowl connected to the outer portion of the dome plate; an annular inner cowl connected to the inner portion of the dome plate; and, a swirler located between the forward surface of the dome plate and the inner and outer cowls in substantial alignment with each of the openings in the dome plate. Each swirler further includes a forward portion and an aft portion and is retained by at least one tab member located upstream thereof so as to be movable in a radial and axial direction.

Abstract: A multi-staged gas turbine engine fuel supply system includes a plurality of fuel injectors and at least first and second staged fuel injection circuits in each of the fuel injectors. Each of the first and second staged fuel injection circuits includes first and second fuel injection points and at least first and second fuel nozzle valves operable to open at different first and second crack open pressures and controllably connected to the first and second staged fuel injection circuits, respectively. A single fuel supply manifold is connected to all of the fuel nozzle valves. A single fuel signal manifold is controllably connected to all of the first and second fuel nozzle valves. The fuel injector may have a valve housing with one of the first fuel nozzle valves and one of the second fuel nozzle valves contained therein.

Abstract: A combustor dome assembly for a gas turbine engine having a longitudinal centerline axis extending therethrough includes: an annular dome plate having an inner portion, an outer portion, a forward surface, and a plurality of circumferentially spaced openings formed therein, wherein a radial section defined between each of the openings includes a cooling trough formed therein; and, a free floating swirler located upstream of the dome plate in substantial alignment with each of the openings in the dome plate, the swirler including a forward portion and an aft portion. The aft portion of each swirler is configured so as to permit air flow to the cooling trough during operation of the gas turbine engine. In particular, the swirler aft portion includes a substantially annular flange extending adjacent to the forward surface of the dome plate, wherein opposing portions of a circumference for the flange adjacent the radial sections of the dome plate are substantially linear.

Abstract: A multi-staged gas turbine engine fuel supply fuel injector includes at least first and second staged fuel injection circuits having first and second fuel injection points. At least first and second fuel nozzle valves operable to open at different first and second crack open pressures are controllably connected to the first and second staged fuel injection circuits, respectively. A single fuel supply manifold is connected to all of the fuel nozzle valves. A single fuel signal manifold is controllably connected to all of the first and second fuel nozzle valves. The fuel injector includes a valve housing containing the fuel nozzle valves.

Abstract: A gas turbine engine fuel injector conduit includes a single feed strip having a single bonded together pair of lengthwise extending plates. Each of the plates has a single row of widthwise spaced apart and lengthwise extending parallel grooves. Opposing grooves in each of the plates are aligned forming internal fuel flow passages through the strip from an inlet end to an outlet end. The feed strip includes a substantially straight middle portion between the inlet end and the outlet end. In one alternative, the middle portion has a radius of curvature greater than a length of the middle portion. The feed strip has at least one acute bend between the inlet end and the middle portion and a bend between the outlet end and the middle portion. The feed strip has fuel inlet holes in the inlet end connected to the internal fuel flow passages.

Abstract: A fuel injector nozzle for dispensing fuel in the combustion chamber of a gas turbine engine, includes an elongated, multi-layered, convoluted nozzle feed strip having an internal passage for directing fuel through the length of the strip from the inlet end to an outlet end; and a cylindrical, multi-layered fuel dispensing nozzle unitary with the feed strip and fluidly connected to the outlet end of the feed strip for dispensing the fuel. The multi-layered feed strip and nozzle allows complex porting of fuel circuits through the injector. The internal fluid passages through the feed strip and nozzle are formed by etching.

Abstract: A gas turbine engine fuel injector conduit includes a single feed strip having a single bonded together pair of lengthwise extending plates. Each of the plates has a single row of widthwise spaced apart and lengthwise extending parallel grooves. Opposing grooves in each of the plates are aligned forming internal fuel flow passages through the strip from an inlet end to an outlet end. The feed strip includes a substantially straight middle portion between the inlet end and the outlet end. In one alternative, the middle portion has a radius of curvature greater than a length of the middle portion. The feed strip has at least one acute bend between the inlet end and the middle portion and a bend between the outlet end and the middle portion. The feed strip has fuel inlet holes in the inlet end connected to the internal fuel flow passages.

Abstract: A fuel injector nozzle for dispensing fuel in the combustion chamber of a gas turbine engine, includes an elongated, multi-layered, convoluted nozzle feed strip having an internal passage for directing fuel through the length of the strip from the inlet end to an outlet end; and a cylindrical, multi-layered fuel dispensing nozzle unitary with the feed strip and fluidly connected to the outlet end of the feed strip for dispensing the fuel. The multi-layered feed strip and nozzle allows complex porting of fuel circuits through the injector. The internal fluid passages through the feed strip and nozzle are formed by etching.

Abstract: A two-stage fuel nozzle assembly for a gas turbine engine. The primary combustion region is centrally positioned and includes a fuel injector that is surrounded by one or more swirl chambers to provide a fuel air mixture that is ignited to define a first stage combustion zone. A secondary combustion region is provided by an annular housing that surrounds the primary combustion region, and it includes a secondary fuel injector having a radially outwardly directed opening and surrounded by an annular ring that includes openings for providing a swirl chamber for the secondary combustion region. Cooling air is directed angularly between the primary and secondary combustion zones to delay intermixing and thereby allow more complete combustion of the respective zones prior to their coalescing further downstream. The primary combustion region is activated during idle and low engine power conditions and both the primary and secondary combustion regions are activated during high engine power conditions.