Patents by Inventor James P Landers
James P Landers has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220126293Abstract: A fluidic storage device capable of long-term storage of biological, chemical, and biochemical substances, including fluids and solids of a corrosive nature or generally incompatible with traditional reagent storage methods like blister packs. The fluidic device employs a fiber-based substrate which allows the substance to be stored long-term within the structure of the fiber-based substrate through capillary action. The stored substance can be released from the fiber-based substrate and used as needed as a result of active or passive forces incurred on the fluidic device. The storage as described herein will assist in minimizing the hazards associated with performing POI and POC testing by scaling down the required reagent volumes as well as facilitating long-term reagent storage and analysis on a single integrated, portable fluidic device.Type: ApplicationFiled: October 27, 2021Publication date: April 28, 2022Applicant: University of Virginia Patent FoundationInventors: James P. Landers, Killian O'Connell
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Publication number: 20220097042Abstract: A method to extract, amplify and separate nucleic acid in a microfluidic device having a plurality of chambers and channels can include a) introducing cells having nucleic acid to a first chamber of the microfluidic device and subjecting the cells in the first chamber to conditions that lyse the cells. The method can further include b) subjecting the first chamber to centrifugal force, thereby allowing the lysate or a portion thereof having nucleic acid to be distributed to a second chamber through a first channel in the microfluidic device. The method can also include c) combining the lysate or the portion thereof and reagents for amplification of the nucleic acid, thereby providing a second mixture. The method can also include d) subjecting the second chamber to centrifugal force, thereby allowing gas to be expelled from the second mixture.Type: ApplicationFiled: September 7, 2021Publication date: March 31, 2022Inventors: James P. Landers, Jacquelyn A. DuVall, Delphine Le Roux, Brian Root, Daniel Mills, Daniel A. Nelson, An-chi Tsuei, Brandon L. Thompson, Jingyi Li, Christopher Birch
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Patent number: 11207677Abstract: A microfluidic device for the detection of drugs, explosives, chemical warfare, or other substances which is able to directly accept a swab into the device for testing. This device additionally contains on-board reagents to perform colorimetric testing for threshold determination directly in the device. These features are useful in a wide array of situations, such as at security checkpoints, environmental monitoring, clinical analysis, which require testing completely unknown substances and therefore must test for multiple different substances in one test. This is especially useful for police and other law enforcement officials who often must use field-deployable platforms making accurate field-testing critical for safety.Type: GrantFiled: December 19, 2018Date of Patent: December 28, 2021Assignee: University of Virginia Patent FoundationInventors: Shannon Theresa Krauss, James P. Landers
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Patent number: 11135583Abstract: A method to extract, amplify and separate nucleic acid in a microfluidic device having a plurality of chambers and channels can include a) introducing cells having nucleic acid to a first chamber of the microfluidic device and subjecting the cells in the first chamber to conditions that lyse the cells. The method can further include b) subjecting the first chamber to centrifugal force, thereby allowing the lysate or a portion thereof having nucleic acid to be distributed to a second chamber through a first channel in the microfluidic device. The method can also include c) combining the lysate or the portion thereof and reagents for amplification of the nucleic acid, thereby providing a second mixture. The method can also include d) subjecting the second chamber to centrifugal force, thereby allowing gas to be expelled from the second mixture.Type: GrantFiled: October 13, 2016Date of Patent: October 5, 2021Assignee: University of Virginia Patent FoundationInventors: James P. Landers, Jacquelyn A. DuVall, Delphine Le Roux, Brian Root, Daniel Mills, Daniel A. Nelson, An-Chi Tsuei, Brandon L. Thompson, Jingyi Li, Christopher Birch
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Patent number: 10656146Abstract: The present invention relates to methods for detecting polymeric analytes, especially biopolymers, and sensors for detecting the polymeric analytes. The present invention uses magnetic beads in a rotating magnetic field to provide a visual detection of the presence of a polymeric analyte, such as nucleic acids, lipids, polysaccharides, proteins, etc. When a polymeric analyte binds to the magnetic beads, application of a rotating magnetic field to the beads results in unique pinwheel formations. Without the presence of the polymeric analyte, the movement of the magnetic beads induced by the rotating magnetic field differs significantly from the pinwheel formations. The pinwheel, therefore, is used to detect the presence of polymeric analytes.Type: GrantFiled: September 10, 2010Date of Patent: May 19, 2020Assignee: University of Virginia Patent FoundationInventors: James P. Landers, Daniel C. Leslie
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Publication number: 20190275518Abstract: A microfluidic device for the detection of drugs, explosives, chemical warfare, or other substances which is able to directly accept a swab into the device for testing. This device additionally contains on-board reagents to perform colorimetric testing for threshold determination directly in the device. These features are useful in a wide array of situations, such as at security checkpoints, environmental monitoring, clinical analysis, which require testing completely unknown substances and therefore must test for multiple different substances in one test. This is especially useful for police and other law enforcement officials who often must use field-deployable platforms making accurate field-testing critical for safety.Type: ApplicationFiled: December 19, 2018Publication date: September 12, 2019Applicant: University of Virginia Patent FoundationInventors: Shannon Theresa Krauss, James P. Landers
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Publication number: 20190054468Abstract: This disclosure provides for apparatuses, systems and methods for in vitro sample detection. For example in one embodiment, this disclosure provides an automated Pe-toner microfluidic device (and related method) on a centrifugal platform for DNA sample lysis and DNA extraction. A second embodiment provides a system and method for qualitative detection, quantification, and real-time monitoring of nucleic acid amplification products using magnetic bead aggregation inhibition. A third embodiment provides a platform for simultaneous detection of mRNA markers from blood, cell-free semen, sperm, saliva, and vaginal fluid. The third embodiment comprises a system and method that provide for simple, rapid, and fluorescence-free detection of body fluids using mRNA marker amplification and optical detection for mRNA marker analysis with a smart phone with image analysis.Type: ApplicationFiled: October 21, 2016Publication date: February 21, 2019Inventors: James P. LANDERS, Kimberly Renee JACKSON, Daniel MILLS, Gavin T. GARNER, Jacquelyn A. DuVall, Jingyi LI
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Publication number: 20180313765Abstract: A substance determination system comprises a chip comprising: a substrate; a microfluidic channel disposed on the substrate; a reagent enclosed in the microfluidic channel; and a substance inlet to the microfluidic channel; a centrifugal device configured to rotate the chip; and a micro-processor device configured to assess the reagent in the crofluidic channel. A method for identifying a substance comprises: inserting a substance into an inlet in a microfluid detection device having a reagent disposed in a microfluid circuit connected to a mixing domain; spinning the microfluid detection device to move the substance and the reagent to the mixing domain; capturing a digital image of a reaction between the substance and the reagent; analyzing the digital image of the reaction to determine a color parameter; comparing the color parameter to a reference parameter of a reference composition; and assessing the comparison to determine if the substance is the reference composition.Type: ApplicationFiled: October 21, 2016Publication date: November 1, 2018Inventors: James P. Landers, Shannon Theresa Krauss, Nishant Shukla
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Publication number: 20180304253Abstract: A method to extract, amplify and separate nucleic acid in a microfluidic device having a plurality of chambers and channels can include a) introducing cells having nucleic acid to a first chamber of the microfluidic device and subjecting the cells in the first chamber to conditions that lyse the cells. The method can further include b) subjecting the first chamber to centrifugal force, thereby allowing the lysate or a portion thereof having nucleic acid to be distributed to a second chamber through a first channel in the microfluidic device. The method can also include c) combining the lysate or the portion thereof and reagents for amplification of the nucleic acid, thereby providing a second mixture. The method can also include d) subjecting the second chamber to centrifugal force, thereby allowing gas to be expelled from the second mixture.Type: ApplicationFiled: October 13, 2016Publication date: October 25, 2018Inventors: James P. Landers, Jacquelyn A. DuVall, Delphine Le Roux, Brian Root, Daniel MIlls, Daniel A. Nelson, An-chi Tsuei, Brandon L. Thompson, Jingyi Li, Christopher Birch
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Patent number: 9987576Abstract: A mechanical input to a fluidic filter network can be actuated. The fluidic filter network can include respective branches fluidically coupling the mechanical input to respective deformable mechanical outputs. A mechanical displacement can be selectively coupled a selected deformable mechanical output of the fluidic filter network to a deformable mechanical input of a microfluidic device. A fluid flow in a portion of the microfluidic device can be controlled using the displacement, the selected deformable mechanical output can be selected at least in part by actuating the mechanical input to produce a displacement having energy in a specified range of frequencies, and the fluidic filter network is generally fluidically isolated from the microfluidic device.Type: GrantFiled: December 10, 2013Date of Patent: June 5, 2018Assignees: University of Virginia Patent Foundation, The Regents of the University of CaliforniaInventors: James P. Landers, Matthew R. Begley
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Patent number: 9656261Abstract: Aspects of the disclosure provide a microfluidic chip to facilitate DNA analysis. The microfluidic chip includes a first domain configured for polymerase chain reaction (PCR) amplification of DNA fragments, a dilution domain coupled to the first domain to dilute a PCR mixture received from the first domain, and a second domain that is coupled to the dilution domain so as to receive the amplified DNA fragments. The second domain includes a separation channel that is configured to perform electrophoretic separation of the amplified DNA fragments. In addition, the disclosure provides a DNA analyzer to act on the microfluidic chip to perform an integrated single chip DNA analysis.Type: GrantFiled: March 4, 2011Date of Patent: May 23, 2017Assignees: LEIDOS INNOVATIONS TECHNOLOGY, INC., ZyGEM CORPORATION LTD., UNIVERSITY OF VIRGINIA PATENT FOUNDATIONInventors: H. Randall Bell, Joan M Bienvenue, John W Pettit, James P Landers, Jessica V Norris, Orion N Scott, Daniel J Marchiarullo, Daniel C Leslie
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Patent number: 9649631Abstract: Aspects of the disclosure provide a microfluidic chip. The microfluidic chip includes a first domain configured for polymerase chain reaction (PCR) amplification of DNA fragments, and a second domain for electrophoretic separation. The first domain includes at least a first reaction reservoir designated for PCR amplification based on a first sample, and a second reaction reservoir designated for PCR amplification based on a second sample. The second domain includes at least a first separation unit coupled to the first reaction reservoir to received first amplified DNA fragments based on the first sample, and a second separation unit coupled to the second reaction reservoir to received second amplified DNA fragments based on the second sample. The first separation unit is configured to perform electrophoretic separation for the first amplified DNA fragments, and the second separation unit is configured to perform electrophoretic separation for the second amplified DNA fragments.Type: GrantFiled: March 4, 2011Date of Patent: May 16, 2017Assignee: LEIDOS INNOVATIONS TECHNOLOGY, INC.Inventors: Joan M Bienvenue, James P Landers, Orion N Scott
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Publication number: 20150314223Abstract: A mechanical input to a fluidic filter network can be actuated. The fluidic filter network can include respective branches fluidically coupling the mechanical input to respective deformable mechanical outputs. A mechanical displacement can be selectively coupled a selected deformable mechanical output of the fluidic filter network to a deformable mechanical input of a microfluidic device. A fluid flow in a portion of the microfluidic device can be controlled using the displacement, the selected deformable mechanical output can be selected at least in part by actuating the mechanical input to produce a displacement having energy in a specified range of frequencies, and the fluidic filter network is generally fluidically isolated from the microfluidic device.Type: ApplicationFiled: December 10, 2013Publication date: November 5, 2015Inventors: James P. LANDERS, Matthew R. BEGLEY
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Patent number: 9067207Abstract: Aspects of the disclosure provides a DNA analyzer to facilitate an integrated single-chip DNA analysis. The DNA analyzer includes an interface for coupling a microfluidic chip to the DNA analyzer. The microfluidic chip includes a first domain configured for polymerase chain reaction (PCR) amplification of DNA fragments, and a second domain fluidically coupled to the first domain to receive the DNA fragments and perform electrophoretic separation of the DNA fragments. The DNA fragments are tagged with fluorescent labels. The DNA analyzer includes a detection module to excite the fluorescent labels to emit fluorescence and detect the emitted fluorescence. The detection module includes a laser source, a set of optical elements, a filter module and a photo-detector.Type: GrantFiled: March 4, 2011Date of Patent: June 30, 2015Assignees: UNIVERSITY OF VIRGINIA PATENT FOUNDATION, ZYGEM CORPORATION LTD., Lockheed Martin CorporationInventors: H. Randall Bell, John W Pettit, James P Landers, Joan M Bienvenue, Daniel J Marchiarullo, Brian E Root, Orion N Scott
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Patent number: 9050596Abstract: The present invention relates to microfluidic systems and methods for controlling the flow of fluid using passive components engineered into the microchannels. These passive flow components include fluidic diodes, fluidic capacitors, and fluidic inductors. Various fluidic circuits are provided to control fluid flow including fluid rectifiers, fluid band pass filters, and fluid timers.Type: GrantFiled: May 17, 2012Date of Patent: June 9, 2015Assignee: University of Virginia Patent FoundationInventors: Christopher J. Easley, James M. Karlinsey, James P. Landers, Dan Leslie, Matthew R. Begley
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Patent number: 9044735Abstract: The present invention relates to the fabrication of a grafted, UV photo-polymerized silica-based monolithic column and the use of such column for the extraction of DNA. In one embodiment, a method is provided for fabricating a silica-based monolithic column, wherein a vessel is filled with a polymerization mixture that is formed into monolithic solid phase for DNA extraction through in situ photo-polymerization.Type: GrantFiled: September 28, 2010Date of Patent: June 2, 2015Assignee: University of Virginia Patent FoundationInventors: Jian Wen, Jerome P. Ferrance, James P. Landers
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Publication number: 20150093838Abstract: A microfluidic device having a chip defining fluid channels and having toner patches printed within the channels. The toner patches are printed with hydrophobic toner to apply inertial pressure to fluids travelling through the channels. The density of hydrophobic toner and the dimensions of the toner patch can be varied to alter the inertial pressure applied to the fluid. The chip can be rotated about a rotational axis to apply external pressure to fluids sufficient to overcome the inertial pressure created by the toner patch to push fluid past the toner patch. The rotational speed of the chip can be varied to facilitate movement of fluid through the channels and to push fluid past the hydrophobic toner patches.Type: ApplicationFiled: October 1, 2014Publication date: April 2, 2015Inventors: James P. Landers, Yiwen Ouyang, Jingyi Li
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Patent number: 8961764Abstract: A DNA analyzer includes an interface for coupling a microfluidic chip to the DNA analyzer. The microfluidic chip includes a first separation channel for electrophoretic separation of DNA fragments in a first sample. Further, the DNA analyzer includes a first optical device. The first optical device includes an illuminating path and a detecting path. The illuminating path directs a first input light beam received from a light source to a first separation channel of the microfluidic chip. The first input light beam causes fluorescent labels attached on DNA fragments in the first separation channel to emit a first fluorescence light. The detecting path collects and directs the first fluorescent light to a first plurality of optical fibers. Further, the DNA analyzer includes a spectrometer configured to receive the first fluorescent light from the plurality of optical fibers and detect fluorescent components in the first fluorescent light.Type: GrantFiled: October 14, 2011Date of Patent: February 24, 2015Assignees: Lockheed Martin Corporation, ZyGEM Corporation, Ltd.Inventors: Peter Karl Trost, Michael E. Egan, Doug South, Brian E. Root, Orion N. Scott, James P. Landers
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Patent number: 8916375Abstract: The present invention provides an integrated microfluidic analysis system. The system contains at least a first (pre-reaction treatment) domain for treating a sample prior to subjecting the sample to a chemical reaction. The following domains are optionally added to the first domain: a second (reaction) domain for reacting the chemical of interest in the sample; and a third (post-reaction separation) domain for separating products and reactants coming out of the reaction domain. The integrated microfluidic analysis system of the present invention is most applicable to PCR analysis.Type: GrantFiled: October 12, 2006Date of Patent: December 23, 2014Assignee: University of Virginia Patent FoundationInventors: James P. Landers, Joan Marie Bienvenue, Lindsay Ann Legendre, Christopher J. Easley, James M. Karlinsey
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Publication number: 20130203045Abstract: The invention provides methods to detect or determine the presence or amount of a pathogen, such as a virus or bacterium, in a sample or the amount of cells based on the detection of their genomic DNA. The method employs magnetic substrates and subjects the sample and the magnetic substrate to forms of energy so as to induce aggregate formation and detects the aggregates.Type: ApplicationFiled: May 26, 2011Publication date: August 8, 2013Applicant: University of Virginia Patent FoundationInventors: James P. Landers, Kimberly A. Kelly, Jingyi Li, Daniel C. Leslie