Abstract: A filter holder, a filtration system, a method of filtering a fluid, and a method of manufacturing a filtration system are provided. A filter holder includes a filter holder base having an aperture extending therethrough and a cavity formed at a first end thereof. The filter holder base is shaped to receive and support a backing on a first surface thereof and to receive and support a filter membrane on a second surface thereof. The filter holder includes a seal assembly configured to be coupled to the filter holder base and to sealingly engage a surface of the filter membrane when the filter membrane is on the second surface. The filter holder further includes a cover having an aperture formed therethrough and configured to be disposed over the seal assembly and over at least a portion of the filter holder base and secured to the filter holder base.