Patents by Inventor James R. Henriksen

James R. Henriksen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11981907
    Abstract: Methods and compositions are provided which allow for genetic modification of host cells including, plants and plant cells. The various methods and composition employ a recombinant DNA construct comprising SEQ ID NO: 1 and/or 2 or active variants and fragments thereof. Such polynucleotides find use in facilitating integration of polynucleotides of interest into the DNA N of a host cell, including a plant or plant cell. Vectors, host cells, bacterium and plants comprising the recombinant DNA construct or fragments thereof are provided. Further provided are methods of introducing into a host cell or a plant cell a polynucleotide of interest. The method comprises contacting the host cell with a bacterium competent for the transformation of the host cell, wherein the bacterium comprises a transformation vector comprising a recombinant DNA construct.
    Type: Grant
    Filed: January 19, 2021
    Date of Patent: May 14, 2024
    Assignee: AgBiome, Inc.
    Inventors: Vadim Beilinson, James R. Henriksen
  • Patent number: 11041158
    Abstract: Methods for making a synthetic gene are provided. The methods find use in optimizing a candidate gene nucleic acid sequence for expression in a selected target expression system. The method identifies stable or retained sequences in the candidate gene nucleic acid sequence, identifies disallowed sequences, develops a statistical model based on a whole genome, a partial genome, or transcriptome sequences of the target expression system, generates an optimized candidate gene nucleic acid sequence for use in the target expression system, and makes a synthetic gene comprising the optimized candidate gene nucleic acid sequence. The method allows for optimization of the candidate gene nucleic acid sequence without removing certain stable or retained sequences. Rather, the activity of these sites is positionally modulated or inactivated through upstream and downstream modifications of codons and/or sequence patterns.
    Type: Grant
    Filed: December 21, 2015
    Date of Patent: June 22, 2021
    Assignee: AgBiome, Inc.
    Inventor: James R. Henriksen
  • Publication number: 20210172008
    Abstract: Compositions and methods for isolating new variants of known clustered regularly-interspaced short palindromic repeat (CRISPR) RNA-guided nuclease (RGN) genes and new CRISPR systems are provided. The methods find use in identifying CRISPR RGN gene variants in complex mixtures. Compositions comprise hybridization baits that hybridize to CRISPR RGN genes of interest in order to selectively enrich variant polynucleotides from complex mixtures. Bait sequences may be specific for a number of CRISPR RGN genes from distinct gene families of interest and may be designed to cover each CRISPR RGN gene of interest by at least 2-fold. Bait pools may also comprise baits for sequences flanking CRISPR RGN genes of interest to allow for the identification of tracrRNAs corresponding to novel CRISPR RGN variants and a complete CRISPR system comprising a CRISPR RGN and its associated guide RNA.
    Type: Application
    Filed: April 3, 2019
    Publication date: June 10, 2021
    Applicant: LIFEEDIT, Inc.
    Inventors: Alexandra Briner Crawley, James R. Henriksen, Mark Moore, Rebecca E. Thayer
  • Patent number: 10947556
    Abstract: Methods and compositions are provided which allow for genetic modification of host cells including, plants and plant cells. The various methods and composition employ a recombinant DNA construct comprising SEQ ID NO: 1 and/or 2 or active variants and fragments thereof. Such polynucleotides find use in facilitating integration of polynucleotides of interest into the DNA of a host cell, including a plant or plant cell. Vectors, host cells, bacterium and plants comprising the recombinant DNA construct or fragments thereof are provided. Further provided are methods of introducing into a host cell or a plant cell a polynucleotide of interest. The method comprises contacting the host cell with a bacterium competent for the transformation of the host cell, wherein the bacterium comprises a transformation vector comprising a recombinant DNA construct.
    Type: Grant
    Filed: December 18, 2015
    Date of Patent: March 16, 2021
    Assignee: AgBiome, Inc.
    Inventors: Vadim Beilinson, James R. Henriksen
  • Publication number: 20190316145
    Abstract: Compositions and methods comprising polynucleotides and polypeptides that confer glufosinate resistance to a host cell are provided. Further provided are nucleic acid constructs, host cells, plants, plant cells, explants, seeds and grain having the sequence that confer glufosinate resistance. Various methods of employing these sequences are provided. Such methods include, for example, methods for producing a host cell, plant, plant cell, explant or seed having glufosinate resistance, and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein.
    Type: Application
    Filed: May 17, 2019
    Publication date: October 17, 2019
    Applicant: AgBiome, Inc.
    Inventors: Vadim Beilinson, James R. Henriksen, Janice C. Jones, Rebekah Deter Kelly, Amy Shekita
  • Patent number: 10344295
    Abstract: Compositions and methods comprising polynucleotides and polypeptides that confer glufosinate resistance to a host cell are provided. Further provided are nucleic acid constructs, host cells, plants, plant cells, explants, seeds and grain having the sequence that confer glufosinate resistance. Various methods of employing these sequences are provided. Such methods include, for example, methods for producing a host cell, plant, plant cell, explant or seed having glufosinate resistance, and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein.
    Type: Grant
    Filed: December 18, 2015
    Date of Patent: July 9, 2019
    Assignee: AGBIOME, INC.
    Inventors: Vadim Beilinson, James R. Henriksen, Janice C. Jones, Rebekah Deter Kelly, Amy Shekita
  • Publication number: 20170369890
    Abstract: Methods for making a synthetic gene are provided. The methods find use in optimizing a candidate gene nucleic acid sequence for expression in a selected target expression system. The method identifies stable or retained sequences in the candidate gene nucleic acid sequence, identifies disallowed sequences, develops a statistical model based on a whole genome, a partial genome, or transcriptome sequences of the target expression system, generates an optimized candidate gene nucleic acid sequence for use in the target expression system, and makes a synthetic gene comprising the optimized candidate gene nucleic acid sequence. The method allows for optimization of the candidate gene nucleic acid sequence without removing certain stable or retained sequences. Rather, the activity of these sites is positionally modulated or inactivated through upstream and downstream modifications of codons and/or sequence patterns.
    Type: Application
    Filed: December 21, 2015
    Publication date: December 28, 2017
    Inventor: James R. Henriksen
  • Publication number: 20170369898
    Abstract: Methods and compositions are provided which allow for genetic modification of host cells including, plants and plant cells. The various methods and composition employ a recombinant DNA construct comprising SEQ ID NO: 1 and/or 2 or active variants and fragments thereof. Such polynucleotides find use in facilitating integration of polynucleotides of interest into the DNA of a host cell, including a plant or plant cell. Vectors, host cells, bacterium and plants comprising the recombinant DNA construct or fragments thereof are provided. Further provided are methods of introducing into a host cell or a plant cell a polynucleotide of interest. The method comprises contacting the host cell with a bacterium competent for the transformation of the host cell, wherein the bacterium comprises a transformation vector comprising a recombinant DNA construct.
    Type: Application
    Filed: December 18, 2015
    Publication date: December 28, 2017
    Inventors: Vadim Beilinson, James R. Henriksen
  • Publication number: 20170372004
    Abstract: Methods for classifying organisms using whole or partial genome sequence data are provided. The methods use DNA or protein sequence data and clustering algorithms to assign organisms a protein morphotype (‘pmorph’) based on a given clustering parameter. Pmorphs can be generated quickly from whole genome sequence data, even at draft sequence quality levels, and can resolve fine differences between related organisms. In this manner, organisms can be classified and duplicate organisms within a collection can be identified.
    Type: Application
    Filed: December 17, 2015
    Publication date: December 28, 2017
    Inventors: James R. Henriksen, Daniel J. Tomso
  • Publication number: 20170362604
    Abstract: Compositions and methods comprising polynucleotides and polypeptides that confer glufosinate resistance to a host cell are provided. Further provided are nucleic acid constructs, host cells, plants, plant cells, explants, seeds and grain having the sequence that confer glufosinate resistance. Various methods of employing these sequences are provided. Such methods include, for example, methods for producing a host cell, plant, plant cell, explant or seed having glufosinate resistance, and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein.
    Type: Application
    Filed: December 18, 2015
    Publication date: December 21, 2017
    Inventors: Vadim Beilinson, James R. Henriksen, Janice C. Jones, Rebekah Deter Keliy, Amy Shekita