Abstract: The invention described herein relates to photobioreactors, methods, assembly and use of such apparatus for culturing light-capturing organisms in a cost-effective manner. Various embodiments provide for a passive thermal regulation system employing selected microorganisms in a photobioreactor apparatus and methods for biological production of various fuel and chemical products from these organisms. Additional embodiments provide a solar biofactory system capable of culturing light capturing organisms to an areal productivity of 3.3 g/m2/hr. Further embodiments are directed to a photobioreactor capable of culturing light capturing organisms to an OD730 of about 14 g/L DCW. Such embodiments incorporate passive thermal regulation and systems.