Patents by Inventor James R. Prudent
James R. Prudent has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8361973Abstract: The present invention provides glycoside compounds, methods of preparing such compounds, pharmaceutical compositions comprising such compounds, and a method for the treatment of hyperproliferative diseases using the same.Type: GrantFiled: August 7, 2009Date of Patent: January 29, 2013Assignee: Centrose, LLCInventors: Mohammed S. Shekhani, James R. Prudent, Jill Hutchinson Bollettieri
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Publication number: 20120295808Abstract: Solid support assays using non-standard bases are described. A capture oligonucleotide comprising a molecular recognition sequence is attached to a solid support and hybridized with a target. In some instances, the molecular recognition sequence includes one or more non-standard bases and hybridizes to a complementary tagging sequence of the target oligonucleotide. In other instances, incorporation of a non-standard base (e.g., via PCR or ligation) is used in the assay.Type: ApplicationFiled: May 23, 2012Publication date: November 22, 2012Inventors: Jennifer K. Grenier, David J. Marshall, James R. Prudent, Craig S. Richmond, Eric B. Roesch, Christopher W. Scherrer, Christopher B. Sherrill, Jerod L. Ptacin
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Patent number: 8313932Abstract: The present invention provides nucleic acid based polymerase inhibitors and methods for reducing non-specific polymerase extension and amplification in nucleic acid amplification reactions. The polymerase inhibitors provide a double stranded nucleic acid portion that is recognized by a polymerase enzyme as a template for extension but is incapable of being extended by the polymerase enzyme. The polymerase binds to the polymerase inhibitor which sequesters the enzyme until the temperature achieves a level that denatures the double stranded portion of the inhibitor after which the polymerase is released and can then catalyze nucleic acid extension.Type: GrantFiled: September 17, 2010Date of Patent: November 20, 2012Assignee: Luminex CorporationInventors: Michael James Moser, David J. Marshall, James R. Prudent, Cristopher V. Van Hout, Christine A. Larsen
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Patent number: 8217160Abstract: Solid support assays using non-standard bases are described. A capture oligonucleotide comprising a molecular recognition sequence is attached to a solid support and hybridized with a target. In some instances, the molecular recognition sequence includes one or more non-standard bases and hybridizes to a complementary tagging sequence of the target oligonucleotide. In other instances, incorporation of a non-standard base (e.g., via PCR or ligation) is used in the assay.Type: GrantFiled: July 20, 2006Date of Patent: July 10, 2012Assignee: Eragen Biosciences, Inc.Inventors: Jennifer K. Grenier, David J. Marshall, James R. Prudent, Craig S. Richmond, Eric B. Roesch, Christopher W. Scherrer, Christopher B. Sherrill, Jerod L. Ptacin
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Publication number: 20110319350Abstract: The present invention provides glycoside compounds, methods of preparing such compounds, pharmaceutical compositions comprising such compounds, and a method for the treatment of hyperproliferative diseases using the same.Type: ApplicationFiled: August 7, 2009Publication date: December 29, 2011Applicant: CENTROSE, LLCInventors: Charles R. Hutchinson, Mohammed S. Shekhani, James R. Prudent
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Publication number: 20110104678Abstract: The present invention provides nucleic acid based polymerase inhibitors and methods for reducing non-specific polymerase extension and amplification in nucleic acid amplification reactions. The polymerase inhibitors provide a double stranded nucleic acid portion that is recognized by a polymerase enzyme as a template for extension but is incapable of being extended by the polymerase enzyme. The polymerase binds to the polymerase inhibitor which sequesters the enzyme until the temperature achieves a level that denatures the double stranded portion of the inhibitor after which the polymerase is released and can then catalyze nucleic acid extension.Type: ApplicationFiled: September 17, 2010Publication date: May 5, 2011Inventors: Michael James Moser, David J. Marshall, James R. Prudent, Cristopher V. Van Hout, Christine A. Larsen
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Publication number: 20110064752Abstract: The present invention relates to, inter alia, extracellular drug conjugates (EDC) in which an antibody or other targeting agent (e.g. a targeting moiety) is linked to a drug through a linker (e.g. a non-cleavable linker). These conjugates are useful in the treatment of disease and/or as a tool in the evaluation of biological systems.Type: ApplicationFiled: September 9, 2010Publication date: March 17, 2011Applicant: CENTROSE, LLCInventors: Charles R. Hutchinson, Jill Hutchinson Bollettieri, James R. Prudent, Jon S. Thorson
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Publication number: 20100291570Abstract: Assays using non-natural bases are described. In one embodiment, the method involves contacting a sample suspected of containing the target nucleic acid with a polymerase and first and second primers; amplifying the target nucleic acid by PCR using the first and second primers to generate an amplification product having a double-stranded region and a single-stranded region that comprises the non-natural base; contacting the sample with a reporter comprising a label and a non-natural base that is complementary to the non-natural base of the single-stranded region; annealing at least a portion of the reporter to the single-stranded region of the amplification product; and correlating a signal of the label with the presence of the target nucleic acid in the sample. The invention also provides corresponding kits for use in detecting target nucleic acids in a sample.Type: ApplicationFiled: March 31, 2010Publication date: November 18, 2010Inventors: DAVID J. MARSHALL, JAMES R. PRUDENT, CHRISTOPHER B. SHERRILL, GIDEON SHAPIRO, JENNIFER K. GRENIER, CRAIG S. RICHMOND, SIMONA JURCZYK, JEROD L. PTACIN
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Patent number: 7829314Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.Type: GrantFiled: June 2, 2009Date of Patent: November 9, 2010Assignee: Third Wave Technologies, Inc.Inventors: James R. Prudent, Jeff G. Hall, Victor I. Lyamichev, Mary Ann D. Brow, James E. Dahlberg
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Patent number: 7824862Abstract: The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acid sequences and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).Type: GrantFiled: September 30, 2008Date of Patent: November 2, 2010Assignee: Third Wave Technologies, Inc.Inventors: Fang Dong, Victor I. Lyamichev, James R. Prudent, Lance Fors, Bruce P. Neri, Mary Ann D. Brow, Todd A. Anderson, James E. Dahlberg
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Patent number: 7820808Abstract: The present invention provides nucleic acid based polymerase inhibitors and methods for reducing non-specific polymerase extension and amplification in nucleic acid amplification reactions. The polymerase inhibitors provide a double stranded nucleic acid portion that is recognized by a polymerase enzyme as a template for extension but is incapable of being extended by the polymerase enzyme. The polymerase binds to the polymerase inhibitor which sequesters the enzyme until the temperature achieves a level that denatures the double stranded portion of the inhibitor after which the polymerase is released and can then catalyze nucleic acid extension.Type: GrantFiled: April 1, 2004Date of Patent: October 26, 2010Assignee: EraGen Biosciences, Inc.Inventors: Michael James Moser, David J. Marshall, James R. Prudent, Christopher V. Van Hout, Christine A. Larsen
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Patent number: 7741471Abstract: The present invention provides the combination of the O-2 diphenylcarbamoyl (“DPC”) and N-6 dimethylaminomethylidene (“DMF”) protecting groups for isoguanosine nucleosides that can be utilized in oligonucleotide synthesis.Type: GrantFiled: May 26, 2005Date of Patent: June 22, 2010Assignee: EraGen Biosciences, Inc.Inventors: James R. Prudent, Christopher B. Sherrill
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Publication number: 20100086908Abstract: The present invention relates generally to the field of nucleic acid detection and more specifically to the detection of human respiratory viruses in a patient sample. In some aspects, the invention relates to the detection of multiple respiratory viral groups, including rhinovirus, respiratory syncytial virus, parainfluenza virus, influenza virus, metapneumovirus, adenovirus, coronavirus, and enterovirus.Type: ApplicationFiled: May 16, 2008Publication date: April 8, 2010Inventors: James R. Prudent, David J. Marshall, Wai-Ming Lee, James Gern, Michael J. Moser, Edward L. Beaty, Gerda Harms
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Patent number: 7678542Abstract: The present invention relates to novel phosphoramidites, including positive and neutrally charged compounds. The present invention also provides charge tags for attachment to materials including solid supports and nucleic acids, wherein the charge tags increase or decrease the net charge of the material. The present invention further provides methods for separating and characterizing molecules based on the charge differentials between modified and unmodified materials.Type: GrantFiled: June 3, 2008Date of Patent: March 16, 2010Assignee: Third Wave Technologies, Inc.Inventors: Victor Lyamichev, Zbigniev Skrzypczynski, Hatim T. Allawi, Sarah R. Wayland, Tsetska Y. Takova, Bruce P. Neri, James R. Prudent, Jeff G. Hall
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Publication number: 20090305283Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.Type: ApplicationFiled: June 2, 2009Publication date: December 10, 2009Applicant: THIRD WAVE TECHNOLOGIES, INC.Inventors: James R. Prudent, Jeff G. Hall, Victor I. Lyamichev, Mary Ann D. Brow, James E. Dahlberg
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Patent number: 7588891Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.Type: GrantFiled: July 15, 2005Date of Patent: September 15, 2009Assignee: Third Wave Technologies, Inc.Inventors: James R. Prudent, Jeff G. Hall, Victor I. Lyamichev, Mary Ann D. Brow, James E. Dahlberg
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Publication number: 20090226926Abstract: Assays using non-natural bases are described. In one embodiment, the method involves contacting a sample suspected of containing the target nucleic acid with a polymerase and first and second primers; amplifying the target nucleic acid by PCR using the first and second primers to generate an amplification product having a double-stranded region and a single-stranded region that comprises the non-natural base; contacting the sample with a reporter comprising a label and a non-natural base that is complementary to the non-natural base of the single-stranded region; annealing at least a portion of the reporter to the single-stranded region of the amplification product; and correlating a signal of the label with the presence of the target nucleic acid in the sample. The invention also provides corresponding kits for use in detecting target nucleic acids in a sample.Type: ApplicationFiled: May 1, 2009Publication date: September 10, 2009Inventors: David J. Marshall, James R. Prudent, Christopher B. Sherrill, Gideon Shapiro, Jennifer K. Grenier, Craig S. Richmond, Simona Jurczyk, Jerod L. Ptacin
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Patent number: 7541145Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.Type: GrantFiled: July 15, 2005Date of Patent: June 2, 2009Assignee: Third Wave Technologies, Inc.Inventors: James R. Prudent, Jeff G. Hall, Victor I. Lyamichev, Mary Ann D. Brow, James E. Dahlberg
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Patent number: 7541147Abstract: Assays using non-natural bases are described. In one embodiment, the method involves contacting a sample suspected of containing the target nucleic acid with a polymerase and first and second primers; amplifying the target nucleic acid, if present in the sample, by PCR using the first and second primers to generate an amplification product having a double-stranded region and a single-stranded region that comprises the non-natural base; contacting the sample with a reporter comprising a label and a non-natural base that is complementary to the non-natural base of the single-stranded region; annealing at least a portion of the reporter to the single-stranded region of the amplification product; and correlating a signal of the label with the presence of the target nucleic acid in the sample. The invention also provides corresponding kits for use in detecting target nucleic acids in a sample.Type: GrantFiled: October 12, 2006Date of Patent: June 2, 2009Assignee: EraGen Biosciences, Inc.Inventors: David J. Marshall, James R. Prudent, Christopher B. Sherrill, Gideon Shapiro, Simona Jurczyk, Jerod L. Ptacin
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Publication number: 20090117576Abstract: The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acid sequences and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).Type: ApplicationFiled: September 30, 2008Publication date: May 7, 2009Applicant: THIRD WAVE TECHNOLOGIES, INC.Inventors: Fang Dong, Victor I. Lyamichev, James R. Prudent, Lance Fors, Bruce P. Neri, Mary Ann D. Brow, Todd A. Anderson, James E. Dahlberg