Patents by Inventor James R. Ryland

James R. Ryland has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Manufacturing Methods To Control C-Terminal Lysine, Galactose And Sialic Acid Content In Recombinant Proteins
    Publication number: 20220089712
    Abstract: Provided herein is a method for producing an antibody, such as an anti-TNF? antibody (e.g., infliximab) having a C-terminal lysine content of about 20% to about 70%, and a sialic acid content of about 1% to about 20%, comprising culturing a zinc-responsive host cell transfected with DNA encoding the antibody in a culture medium comprising at least 0.5 ?M zinc; and controlling the concentration of zinc in the culture medium, thereby producing the antibody.
    Type: Application
    Filed: September 29, 2021
    Publication date: March 24, 2022
    Inventors: Marcel Flikweert, Charles Goochee, Francis C. Maslanka, Franciscus Johannes Ignatius Nagel, James R. Ryland, Eugene Schaefer
  • Manufacturing methods to control C-terminal lysine, galactose and sialic acid content in recombinant proteins
    Patent number: 11149085
    Abstract: Provided herein is a method for producing an antibody, such as an anti-TNF? antibody (e.g., infliximab) having a C-terminal lysine content of about 20% to about 70%, and a sialic acid content of about 1% to about 20%, comprising culturing a zinc-responsive host cell transfected with DNA encoding the antibody in a culture medium comprising at least 0.5 ?M zinc; and controlling the concentration of zinc in the culture medium, thereby producing the antibody.
    Type: Grant
    Filed: December 23, 2015
    Date of Patent: October 19, 2021
    Assignees: JANSSEN BIOTECH, INC., JANSSEN BIOLOGICS B.V.
    Inventors: Marcel Flikweert, Charles Goochee, Francis C. Maslanka, Franciscus Johannes Ignatius Nagel, James R. Ryland, Eugene Schaefer
  • Purification of hemoglobin
    Patent number: 5840851
    Abstract: The present invention generally relates to methods for purifying hemoglobin solutions and to hemoglobin solutions obtained by the methods. In one aspect, such methods include removing contaminants in crude hemoglobin-containing lysates with heat treatment. In a further aspect, the present invention provides methods for producing substantially purified hemoglobin solutions using immobilized metal affinity chromatography, optionally following by anion exchange chromatography.
    Type: Grant
    Filed: May 10, 1995
    Date of Patent: November 24, 1998
    Inventors: J. Jeffrey Plomer, James R. Ryland, Maura-Ann H. Matthews, David W. Traylor, Erin E. Milne, Steven L. Durfee, Antony J. Mathews, Justinian O. Neway
  • Method for the rapid removal of protoporphyrin from protoporphyrin IX-containing solutions of hemoglobin
    Patent number: 5665869
    Abstract: The present invention relates to a method for the production of a substantially protoporphyrin IX free hemoglobin solution comprising: rapidly heating a crude protoporphyrin IX-containing hemoglobin solution for a relatively short time and at a relatively high temperature to reduce protoporphyrin IX-containing hemoglobin to insignificant levels in said protoporphyrin IX-containing hemoglobin solution.
    Type: Grant
    Filed: November 15, 1993
    Date of Patent: September 9, 1997
    Assignee: Somatogen, Inc.
    Inventors: James R. Ryland, Maura-Ann H. Matthews, Ulrich P. Ernst, Daniel E. Houk, David W. Traylor, Lee R. Williams