Abstract: The present invention relates to stable and high-producing site-specific integration (SSI) host cells, e. g. Chinese hamster ovary (CHO)-derived host cells, methods to produce and to use them.

Abstract: Methods for generating mammalian cells characterized by an increased concentration of at least one of miR-15, miR-16, and miR-34 for producing proteins at an industrial scale are provided. Methods for using the mammalian cells, in particular for the production of proteins of interest, are also provided.

Abstract: The present invention relates to stable and high-producing site-specific integration (SSI) host cells, e. g. Chinese hamster ovary (CHO)-derived host cells, methods to produce and to use them.

Abstract: The present invention relates to stable and high-producing site-specific integration (SSI) host cells, e. g. Chinese hamster ovary (CHO)-derived host cells, methods to produce and to use them.

Abstract: The present invention relates to the generation of mammalian cells capable of producing proteins at an industrial scale and means and methods to obtain and use said cells, in particular for protein production.

Abstract: The present invention relates to stable and high-producing site-specific integration (SSI) host cells, e.g. Chinese hamster ovary (CHO)-derived host cells, methods to produce and to use them.

Abstract: The present invention relates to a mammalian cell based expression and secretion system and the expression and secretion of recombinant proteins by using secretory signal peptides. The present invention also relates to an expression cassette useful for the secretion of a heterologous gene from a mammalian cell, in particular a CHO cell. The present invention is also directed to a method of secreting a heterologous protein from mammalian cells such as CHO cells.

Abstract: A hanging planter for germinating seeds to seedlings and growing the seedlings to full size plants where the planter has an opening in its bottom and a retainer plug located within the planter to prevent a layer of soil in the bottom of the planter from falling out when the planter is turned upside down. With the planter upside down, a seed is passed through the opening to the soil in the bottom of the planter and is watered thru the opening in the planter bottom as it grows right side up. While the plant is a seedling the planter is turned from its upside down position to its right side up position and hung above the ground to turn the stalk portion of the seedling from being right side up to being upside side down while the root portion remains located in the planter without having been replanted.

Abstract: The present invention relates to a mammalian cell based expression and secretion system and the expression and secretion of recombinant proteins by using secretory signal peptides. The present invention also relates to an expression cassette useful for the secretion of a heterologous gene from a mammalian cell, in particular a CHO cell. The present invention is also directed to a method of secreting a heterologous protein from mammalian cells such as CHO cells.

Abstract: A mammalian host cell with a high-level expression of recombinant antibody containing a double-gene vector with gene optimized nucleotide sequences encoding the light and the heavy antibody chains. The double-gene vector itself. A process for enhancing the production of recombinant antibody in a mammalian host cell.