Abstract: T cell manufacturing methods that co-culture T cells with autologous cell types to stimulate the T cells during manufacture are described. The T cells express a recombinant receptor. A binding domain expressed by the T cells binds an epitope on stimulating autologous cell types and/or an immune cell activating multi-specific binding molecule (e.g., a bi-specific antibody) during the co-culture. The methods can also allow for the expression of large transgenes by utilizing electroporation and transposons to deliver transgenes encoding the recombinant receptor. The disclosed methods create manufactured T cell populations with a high number of cells in comparison to starting cell numbers, a high percentage of recombinant receptor-expressing T cells within the cell number, and a beneficial naïve T cell marker profile.

Abstract: Cartridges for vaporizer devices are provided. In one exemplary embodiment, the cartridge can include a primary reservoir, a secondary reservoir in fluid communication with the primary reservoir, and a vaporization chamber in communication with the secondary reservoir in which the vaporization chamber includes a first wicking element. The primary reservoir is configured to store a majority fraction of vaporizable material when in a first pressure state and configured to expel the vaporizable material in response to an increase in headspace when in a second pressure state. The secondary reservoir is formed of absorbent material configured to receive a first volume of the vaporizable material from the primary reservoir in the first pressure state and to receive a second volume of the vaporizable material from the primary reservoir in the second pressure state in which the second volume is greater than the first volume. Vaporizer devices are also provided.

Abstract: Cartridges for vaporizer devices are provided. In one exemplary embodiment, the cartridge can include a reservoir housing having a storage chamber and a dispensing chamber, and a vaporization chamber in communication with the dispensing chamber. The storage chamber is configured to hold a first fraction of a vaporizable material and the dispensing chamber is configured to hold a second fraction of the vaporizable material. The dispensing chamber is further configured to selectively dispense at least a first portion of the second fraction of the vaporizable material through at least one dispense opening in response to generation of one or more pressure pulses created within the dispensing chamber. The vaporizable chamber is configured to receive the dispensed vaporizable material from the dispensing chamber for vaporization by a first heating element to form a vaporized material. Vaporizer devices are also provided.

Abstract: Some embodiments of the methods and compositions provided herein include methods and/or systems for increasing an activity of a cell comprising a chimeric antigen receptor (CAR), comprising use of a first nucleic acid encoding a transcription response element (TRE); and a second nucleic acid encoding a CAR, wherein the activity of the cell is increased compared to a cell lacking the first nucleic acid. In some embodiments, the increased activity of the cell is selected from: (i) survival of a subject administered the cell, wherein the subject comprises a target cell comprising an antigen, wherein the CAR is capable of specifically binding to the antigen; (ii) killing of a target cell comprising an antigen, wherein the CAR is capable of specifically binding to the antigen; and (iii) proliferation of the cell in the presence of a target cell comprising an antigen, wherein the CAR is capable of specifically binding to the antigen.

Abstract: Cartridges for vaporizer devices are provided. In one exemplary embodiment, a cartridge can include a reservoir housing that includes a reservoir chamber configured to contain a vaporizable material and first and second orifices each in fluid communication with the reservoir chamber. The first orifice is configured to allow at least a portion of the vaporizable material to be withdrawn from the reservoir chamber in response to generation of a first pressure differential across the first orifice that exceeds a first predetermined threshold pressure differential. The second orifice is configured to allow a portion of ambient air that is outside the reservoir housing to enter the reservoir chamber in response to generation of a second pressure differential across the second orifice that exceeds a second predetermined threshold pressure differential during the withdrawal of the vaporizable material from the reservoir chamber. Vaporizer devices and methods are also provided.

Abstract: Vaporizer devices are provided. In one exemplary embodiment, the vaporizer device can include a vaporizer body and a cartridge selectively coupled to and removable from the vaporizer body. The vaporizer body includes a cartridge receptacle and a heating element disposed within the cartridge receptacle and affixed to the vaporizer body. The cartridge includes a reservoir chamber and a wicking element that is in fluid communication with the reservoir chamber. The wicking element is configured to draw at least a portion of a vaporizable material from the reservoir chamber. The wicking element and at least a portion of the heating element are brought into direct contact with each other such that at least a portion of the vaporizable material received within the wicking element can be substantially vaporized to form vaporized material. Cartridges for vaporizer devices are also provided.

Abstract: Cartridges for vaporizer devices are provided. In one exemplary embodiment, the cartridge can include a primary reservoir, a secondary reservoir in fluid communication with the primary reservoir, and a vaporization chamber in communication with the secondary reservoir in which the vaporization chamber includes a first wicking element. The primary reservoir is configured to store a majority fraction of vaporizable material when in a first pressure state and configured to expel the vaporizable material in response to an increase in headspace when in a second pressure state. The secondary reservoir is formed of absorbent material configured to receive a first volume of the vaporizable material from the primary reservoir in the first pressure state and to receive a second volume of the vaporizable material from the primary reservoir in the second pressure state in which the second volume is greater than the first volume. Vaporizer devices are also provided.

Abstract: A heating element for a vaporizer cartridge is provided. The vaporizer cartridge may include a reservoir containing vaporizable material and a wicking element in fluid communication with the reservoir. The heating element may include a heating portion, a cartridge contact, and a leg. The heating portion includes at least two tines spaced apart from one another. The cartridge contact may be in in electrical communication with a power source. The leg extends between the heating portion and the cartridge contact. The heating portion may be crimped around the wicking element such that the heating portion secures the wicking element to the heating element and contacts at least two surfaces of the wicking element.

Abstract: Cartridges for vaporizer devices are provided. In one exemplary embodiment, the cartridge can include an atomizer and a channel extending through the cartridge from an inlet to an outlet. The atomizer includes a wicking element that is configured to substantially draw a vaporizable material from a reservoir and into the atomizer, and a heating element that is configured to substantially vaporize the vaporizable material into a vaporized material. The channel has an airflow path and a vapor path that intersect at a first junction between the inlet and the outlet. The airflow path is configured to receive and substantially allow air to pass therethrough, and the vapor path is configured to direct the vaporized material into the first junction so that the vaporized material mixes with the air to substantially form an aerosol downstream of the atomizer. Vaporizer devices are also provided.

Abstract: Cartridges for vaporizer devices are provided. In one exemplary embodiment, the cartridge can include an atomizer and a channel extending through the cartridge from an inlet to an outlet. The atomizer includes a wicking element that is configured to substantially draw a vaporizable material from a reservoir and into the atomizer, and a heating element that is configured to substantially vaporize the vaporizable material into a vaporized material. The channel has an airflow path and a vapor path that intersect at a first junction between the inlet and the outlet. The airflow path is configured to receive and substantially allow air to pass therethrough, and the vapor path is configured to direct the vaporized material into the first junction so that the vaporized material mixes with the air to substantially form an aerosol downstream of the atomizer. Vaporizer devices are also provided.

Abstract: A heating element for a vaporizer cartridge is provided. The vaporizer cartridge may include a reservoir containing vaporizable material and a wicking element in fluid communication with the reservoir. The heating element may include a heating portion, a cartridge contact, and a leg. The heating portion includes at least two tines spaced apart from one another. The cartridge contact may be in in electrical communication with a power source. The leg extends between the heating portion and the cartridge contact. The heating portion may be crimped around the wicking element such that the heating portion secures the wicking element to the heating element and contacts at least two surfaces of the wicking element.

Abstract: Cartridges for vaporizer devices are provided. In one exemplary embodiment, the cartridge can include a reservoir housing having a first housing end and a second housing end opposite the first housing end, an airflow tube that extends through the reservoir housing, and first and second seals that are each substantially impermeable to fluid, in which the first seal is substantially secured to the first housing end and the second seal is substantially secured to the second housing end. The reservoir housing is configured to hold vaporizable material and the first and second seals are configured to be selectively compromised to allow access to the vaporizable material within the reservoir housing for vaporization into vaporized material. Vaporizer devices are also provided.

Abstract: Some embodiments of the methods and compositions provided herein relate to chimeric proteins comprising a programmed cell death protein 1 (PD 1) extracellular domain and an intracellular immunostimulatory domain. Some embodiments include a cell containing a PD 1 chimeric polypeptide and a chimeric antigen receptor (CAR). In some embodiments, the CAR comprises a ligand binding domain capable of specifically binding to a target antigen on a solid tumor. More embodiments relate to therapies to treat, inhibit or ameliorate certain disorders, such as a cancer, such as a solid tumor.

Abstract: An electronic smoking device (10) comprises a power supply/atomizer portion (12) and a replaceable liquid reservoir portion (14) which is coupleable with the power supply/atomizer portion (12). The liquid reservoir portion comprises a liquid reservoir (34) storing a liquid and the power supply/atomizer portion comprises a power supply (18) and an atomizer (26) adapted to atomize the liquid stored in the liquid reservoir when operated by the power supply. The power supply/atomizer portion comprises a penetrating element (40) that is configured to penetrate the liquid reservoir when the power supply/atomizer portion is coupled with the liquid reservoir portion, and a wick (30). The wick (30) is attached to the penetrating element and is configured so that a portion (31) of the wick entering the liquid reservoir when the power supply/atomizer portion is coupled with the liquid reservoir portion has a U-shape.

Abstract: Cartridges for vaporizer devices are provided. In one exemplary embodiment, the cartridge can include a reservoir housing having a storage chamber and a dispensing chamber, and a vaporization chamber in communication with the dispensing chamber. The storage chamber is configured to hold a first fraction of a vaporizable material and the dispensing chamber is configured to hold a second fraction of the vaporizable material. The dispensing chamber is further configured to selectively dispense at least a first portion of the second fraction of the vaporizable material through at least one dispense opening in response to generation of one or more pressure pulses created within the dispensing chamber. The vaporizable chamber is configured to receive the dispensed vaporizable material from the dispensing chamber for vaporization by a first heating element to form a vaporized material. Vaporizer devices are also provided.

Abstract: Cartridges for vaporizer devices are provided. In one exemplary embodiment, the cartridge can include a reservoir being configured to contain a liquid vaporizable material that includes at least one ionic component, an airflow tube that extends though the reservoir and defines an airflow passageway therethrough, and first and second electrodes. The airflow tube includes a wicking element that is in fluid communication with the reservoir, in which the wicking element is configured to substantially draw at least a portion of the liquid vaporizable material from the reservoir into the airflow passageway. The first and second electrodes are positioned substantially on or adjacent to opposite surfaces of the wicking element, in which the liquid vaporizable material received within the wicking element is substantially vaporized in response to generation of a potential difference between the first and second electrodes. Vaporizer devices are also provided.

Abstract: Cartridges for vaporizer devices are provided. In one exemplary embodiment, the cartridge can include a reservoir configured to contain a plurality of encapsulated particles and an airflow tube extending through the reservoir. Each of the plurality of encapsulated particles includes a core formed of a liquid vaporizable material and a coating material that forms a shell surrounding the core, in which the shells of the plurality of encapsulated particles are configured to be selectively ruptured to release the liquid vaporizable material therefrom. The airflow tube includes a wicking element that is in communication with the reservoir, in which the wicking element is configured to draw, into the airflow tube for vaporization, at least a portion of the liquid vaporizable material that is released by rupturing one or more shells of the plurality of encapsulated particles. Vaporizer devices are also provided.

Abstract: Cartridges for vaporizer devices are provided. In one exemplary embodiment, the cartridge can include an atomizer and a channel extending through the cartridge from an inlet to an outlet. The atomizer includes a wicking element that is configured to substantially draw a vaporizable material from a reservoir and into the atomizer, and a heating element that is configured to substantially vaporize the vaporizable material into a vaporized material. The channel has an airflow path and a vapor path that intersect at a first junction between the inlet and the outlet. The airflow path is configured to receive and substantially allow air to pass therethrough, and the vapor path is configured to direct the vaporized material into the first junction so that the vaporized material mixes with the air to substantially form an aerosol downstream of the atomizer. Vaporizer devices are also provided.

Abstract: Cartridges for vaporizer devices are provided. In one exemplary embodiment, the cartridge can include a reservoir housing having a storage chamber and a dispensing chamber, and a vaporization chamber in communication with the dispensing chamber. The storage chamber is configured to hold a first fraction of a vaporizable material and the dispensing chamber is configured to hold a second fraction of the vaporizable material. The dispensing chamber is further configured to selectively dispense at least a first portion of the second fraction of the vaporizable material through at least one dispense opening in response to generation of one or more pressure pulses created within the dispensing chamber. The vaporizable chamber is configured to receive the dispensed vaporizable material from the dispensing chamber for vaporization by a first heating element to form a vaporized material. Vaporizer devices are also provided.

Abstract: A vaporizer device may include a shell, a cartridge receptacle, and a skeleton. The cartridge receptacle may be formed from a cartridge interface disposed at least partially inside a sheath. The cartridge interface may be configured to provide a plurality of electrical couplings with a vaporizer cartridge when the vaporizer cartridge is disposed inside the cartridge receptacle. The plurality of electrical couplings may include a first electrical coupling with a heating element of the vaporizer cartridge. The plurality of electrical couplings may further include a second electrical coupling with a cartridge identification chip of the vaporizer cartridge. The skeleton may be coupled with the cartridge interface. The skeleton may be configured to secure the cartridge interface inside the shell.