Abstract: A flow sensor assembly comprising a first substrate, a flow sensor located over the first substrate, a lid located over the first substrate and the flow sensor, a flow inlet channel, a flow outlet channel, and an overmold laterally encircling the flow sensor. The overmold is in contact with the side walls of the flow sensor, and extends between the flow sensor and the lid such that the overmold, the flow sensor, and the lid define a flow sensing channel between the flow inlet channel and the flow outlet channel. the lid and the encapsulation cooperate to define the flow inlet channel and the flow outlet channel.

Abstract: Vaporizer devices are disclosed. In one exemplary embodiment, a vaporizer device can include a vaporizer body including a first airflow path extending at least partially therethrough, and a sensor assembly residing at least partially within the vaporizer body. The sensor assembly includes a flexible sensor that is in communication with the first airflow path. The flexible sensor is configured to reversibly deflect from an initial state to a first state in response to a first user-activated force representing air being drawn through the first airflow path, and configured to reversibly deflect from the initial state to a second state in response to a second user-activated force representing an acceleration of the vaporizer body. Sensor assemblies for a vaporizer device are also provided.

Abstract: A vaporization device includes a cartridge for a vaporizer device. For example, the vaporizer cartridge and/or features thereof may improve management of leaks of vaporizable material from the vaporizer cartridge, control of airflow within and/or near the vaporizer cartridge, heating of vaporizable material in the vaporizer cartridge, management of condensate in the vaporizer cartridge, and/or other assembly features of the vaporizer cartridge. Related systems, methods, and articles of manufacture are also described.

Abstract: A vaporization device includes a cartridge for a vaporizer device. For example, the vaporizer cartridge and/or features thereof may improve management of leaks of vaporizable material from the vaporizer cartridge, control of airflow within and/or near the vaporizer cartridge, heating of vaporizable material in the vaporizer cartridge, management of condensate in the vaporizer cartridge, and/or other assembly features of the vaporizer cartridge. Related systems, methods, and articles of manufacture are also described.

Abstract: A vaporization device includes a cartridge for a vaporizer device. For example, the vaporizer cartridge and/or features thereof may improve management of leaks of vaporizable material from the vaporizer cartridge, control of airflow within and/or near the vaporizer cartridge, heating of vaporizable material in the vaporizer cartridge, management of condensate in the vaporizer cartridge, and/or other assembly features of the vaporizer cartridge. Related systems, methods, and articles of manufacture are also described.

Abstract: Vaporizer devices are provided. In one exemplary embodiment, the vaporizer device can include a vaporizer body and a cartridge selectively coupled to and removable from the vaporizer body. The vaporizer body includes a cartridge receptacle and a heating element disposed within the cartridge receptacle and affixed to the vaporizer body. The cartridge includes a reservoir chamber and a wicking element that is in fluid communication with the reservoir chamber. The wicking element is configured to draw at least a portion of a vaporizable material from the reservoir chamber. The wicking element and at least a portion of the heating element are brought into direct contact with each other such that at least a portion of the vaporizable material received within the wicking element can be substantially vaporized to form vaporized material. Cartridges for vaporizer devices are also provided.

Abstract: Vaporizer devices are disclosed. In one exemplary embodiment, a vaporizer device can include a vaporizer body including a first airflow path extending at least partially therethrough, and a sensor assembly residing at least partially within the vaporizer body. The sensor assembly includes a flexible sensor that is in communication with the first airflow path. The flexible sensor is configured to reversibly deflect from an initial state to a first state in response to a first user-activated force representing air being drawn through the first airflow path, and configured to reversibly deflect from the initial state to a second state in response to a second user-activated force representing an acceleration of the vaporizer body. Sensor assemblies for a vaporizer device are also provided.

Abstract: Cartridges for vaporizer devices are provided. In one exemplary embodiment, a cartridge can include a reservoir housing that includes a reservoir chamber configured to contain a vaporizable material and first and second orifices each in fluid communication with the reservoir chamber. The first orifice is configured to allow at least a portion of the vaporizable material to be withdrawn from the reservoir chamber in response to generation of a first pressure differential across the first orifice that exceeds a first predetermined threshold pressure differential. The second orifice is configured to allow a portion of ambient air that is outside the reservoir housing to enter the reservoir chamber in response to generation of a second pressure differential across the second orifice that exceeds a second predetermined threshold pressure differential during the withdrawal of the vaporizable material from the reservoir chamber. Vaporizer devices and methods are also provided.

Abstract: T cell manufacturing methods that co-culture T cells with autologous cell types to stimulate the T cells during manufacture are described. The T cells express a recombinant receptor. A binding domain expressed by the T cells binds an epitope on stimulating autologous cell types and/or an immune cell activating multi-specific binding molecule (e.g., a bi-specific antibody) during the co-culture. The methods can also allow for the expression of large transgenes by utilizing electroporation and transposons to deliver transgenes encoding the recombinant receptor. The disclosed methods create manufactured T cell populations with a high number of cells in comparison to starting cell numbers, a high percentage of recombinant receptor-expressing T cells within the cell number, and a beneficial naïve T cell marker profile.

Abstract: Cartridges for vaporizer devices are provided. In one exemplary embodiment, the cartridge can include a primary reservoir, a secondary reservoir in fluid communication with the primary reservoir, and a vaporization chamber in communication with the secondary reservoir in which the vaporization chamber includes a first wicking element. The primary reservoir is configured to store a majority fraction of vaporizable material when in a first pressure state and configured to expel the vaporizable material in response to an increase in headspace when in a second pressure state. The secondary reservoir is formed of absorbent material configured to receive a first volume of the vaporizable material from the primary reservoir in the first pressure state and to receive a second volume of the vaporizable material from the primary reservoir in the second pressure state in which the second volume is greater than the first volume. Vaporizer devices are also provided.

Abstract: Cartridges for vaporizer devices are provided. In one exemplary embodiment, the cartridge can include a reservoir housing having a storage chamber and a dispensing chamber, and a vaporization chamber in communication with the dispensing chamber. The storage chamber is configured to hold a first fraction of a vaporizable material and the dispensing chamber is configured to hold a second fraction of the vaporizable material. The dispensing chamber is further configured to selectively dispense at least a first portion of the second fraction of the vaporizable material through at least one dispense opening in response to generation of one or more pressure pulses created within the dispensing chamber. The vaporizable chamber is configured to receive the dispensed vaporizable material from the dispensing chamber for vaporization by a first heating element to form a vaporized material. Vaporizer devices are also provided.

Abstract: Some embodiments of the methods and compositions provided herein include methods and/or systems for increasing an activity of a cell comprising a chimeric antigen receptor (CAR), comprising use of a first nucleic acid encoding a transcription response element (TRE); and a second nucleic acid encoding a CAR, wherein the activity of the cell is increased compared to a cell lacking the first nucleic acid. In some embodiments, the increased activity of the cell is selected from: (i) survival of a subject administered the cell, wherein the subject comprises a target cell comprising an antigen, wherein the CAR is capable of specifically binding to the antigen; (ii) killing of a target cell comprising an antigen, wherein the CAR is capable of specifically binding to the antigen; and (iii) proliferation of the cell in the presence of a target cell comprising an antigen, wherein the CAR is capable of specifically binding to the antigen.

Abstract: Cartridges for vaporizer devices are provided. In one exemplary embodiment, a cartridge can include a reservoir housing that includes a reservoir chamber configured to contain a vaporizable material and first and second orifices each in fluid communication with the reservoir chamber. The first orifice is configured to allow at least a portion of the vaporizable material to be withdrawn from the reservoir chamber in response to generation of a first pressure differential across the first orifice that exceeds a first predetermined threshold pressure differential. The second orifice is configured to allow a portion of ambient air that is outside the reservoir housing to enter the reservoir chamber in response to generation of a second pressure differential across the second orifice that exceeds a second predetermined threshold pressure differential during the withdrawal of the vaporizable material from the reservoir chamber. Vaporizer devices and methods are also provided.

Abstract: Vaporizer devices are provided. In one exemplary embodiment, the vaporizer device can include a vaporizer body and a cartridge selectively coupled to and removable from the vaporizer body. The vaporizer body includes a cartridge receptacle and a heating element disposed within the cartridge receptacle and affixed to the vaporizer body. The cartridge includes a reservoir chamber and a wicking element that is in fluid communication with the reservoir chamber. The wicking element is configured to draw at least a portion of a vaporizable material from the reservoir chamber. The wicking element and at least a portion of the heating element are brought into direct contact with each other such that at least a portion of the vaporizable material received within the wicking element can be substantially vaporized to form vaporized material. Cartridges for vaporizer devices are also provided.

Abstract: Cartridges for vaporizer devices are provided. In one exemplary embodiment, the cartridge can include a primary reservoir, a secondary reservoir in fluid communication with the primary reservoir, and a vaporization chamber in communication with the secondary reservoir in which the vaporization chamber includes a first wicking element. The primary reservoir is configured to store a majority fraction of vaporizable material when in a first pressure state and configured to expel the vaporizable material in response to an increase in headspace when in a second pressure state. The secondary reservoir is formed of absorbent material configured to receive a first volume of the vaporizable material from the primary reservoir in the first pressure state and to receive a second volume of the vaporizable material from the primary reservoir in the second pressure state in which the second volume is greater than the first volume. Vaporizer devices are also provided.

Abstract: A heating element for a vaporizer cartridge is provided. The vaporizer cartridge may include a reservoir containing vaporizable material and a wicking element in fluid communication with the reservoir. The heating element may include a heating portion, a cartridge contact, and a leg. The heating portion includes at least two tines spaced apart from one another. The cartridge contact may be in in electrical communication with a power source. The leg extends between the heating portion and the cartridge contact. The heating portion may be crimped around the wicking element such that the heating portion secures the wicking element to the heating element and contacts at least two surfaces of the wicking element.

Abstract: Cartridges for vaporizer devices are provided. In one exemplary embodiment, the cartridge can include an atomizer and a channel extending through the cartridge from an inlet to an outlet. The atomizer includes a wicking element that is configured to substantially draw a vaporizable material from a reservoir and into the atomizer, and a heating element that is configured to substantially vaporize the vaporizable material into a vaporized material. The channel has an airflow path and a vapor path that intersect at a first junction between the inlet and the outlet. The airflow path is configured to receive and substantially allow air to pass therethrough, and the vapor path is configured to direct the vaporized material into the first junction so that the vaporized material mixes with the air to substantially form an aerosol downstream of the atomizer. Vaporizer devices are also provided.

Abstract: Cartridges for vaporizer devices are provided. In one exemplary embodiment, the cartridge can include an atomizer and a channel extending through the cartridge from an inlet to an outlet. The atomizer includes a wicking element that is configured to substantially draw a vaporizable material from a reservoir and into the atomizer, and a heating element that is configured to substantially vaporize the vaporizable material into a vaporized material. The channel has an airflow path and a vapor path that intersect at a first junction between the inlet and the outlet. The airflow path is configured to receive and substantially allow air to pass therethrough, and the vapor path is configured to direct the vaporized material into the first junction so that the vaporized material mixes with the air to substantially form an aerosol downstream of the atomizer. Vaporizer devices are also provided.

Abstract: A heating element for a vaporizer cartridge is provided. The vaporizer cartridge may include a reservoir containing vaporizable material and a wicking element in fluid communication with the reservoir. The heating element may include a heating portion, a cartridge contact, and a leg. The heating portion includes at least two tines spaced apart from one another. The cartridge contact may be in in electrical communication with a power source. The leg extends between the heating portion and the cartridge contact. The heating portion may be crimped around the wicking element such that the heating portion secures the wicking element to the heating element and contacts at least two surfaces of the wicking element.

Abstract: Cartridges for vaporizer devices are provided. In one exemplary embodiment, the cartridge can include a reservoir housing having a first housing end and a second housing end opposite the first housing end, an airflow tube that extends through the reservoir housing, and first and second seals that are each substantially impermeable to fluid, in which the first seal is substantially secured to the first housing end and the second seal is substantially secured to the second housing end. The reservoir housing is configured to hold vaporizable material and the first and second seals are configured to be selectively compromised to allow access to the vaporizable material within the reservoir housing for vaporization into vaporized material. Vaporizer devices are also provided.