Abstract: Automated systems and methods for screening individual plants for increased tolerance or sensitivity to various conditions are provided.

Abstract: Automated systems and methods for screening individual plants for increased tolerance or sensitivity to various conditions are provided.

Abstract: Provided are efficient, cost-effective and water tolerant methods (e.g., single-vial methods) for preparing fatty acid esters from organic matter, including: obtaining organic matter having at least one fat substituent, contacting the organic matter in a reaction mixture with a basic solution under conditions suitable to provide for hydrolytic release of monomeric fatty acids from the at least one fat substituent to provide a base-treated reaction mixture, and esterifying the monomeric fatty acids of the base-treated reaction mixture by acidification of the reaction mixture and treating in the presence of an organic alcohol to provide fatty acid esters. The methods optionally further include, prior to esterifying, neutralizing the base-treated reaction mixture to provide for neutralized fatty acids, separating the neutralized fatty acids from the neutralized reaction mixture, and dissolving the separated fatty acids in the esterification reaction mixture.

Abstract: Automated systems and methods for screening individual plants for increased tolerance or sensitivity to various conditions are provided.

Abstract: Provided are efficient, cost-effective and water tolerant methods (e.g., single-vial methods) for preparing fatty acid esters from organic matter, comprising: obtaining organic matter comprising at least one fat substituent, contacting the organic matter in a reaction mixture with a basic solution under conditions suitable to provide for hydrolytic release of monomeric fatty acids from the at least one fat substituent to provide a base-treated reaction mixture, and esterifying the monomeric fatty acids of the base-treated reaction mixture by acidification of the reaction mixture and treating in the presence of an organic alcohol to provide fatty acid esters. The methods optionally further comprise, prior to esterifying, neutralizing the base-treated reaction mixture to provide for neutralized fatty acids, separating the neutralized fatty acids from the neutralized reaction mixture, and dissolving the separated fatty acids in the esterification reaction mixture.

Abstract: This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.

Abstract: Methods for classifying plants by remote sensing and image analysis technology are presented. These methods are useful for evaluating plants and for selecting plants for a plant breeding program which has as its goal to selectively alter phenotype. The methods combine the newer techniques of remote sensing technology to obtain indirect correlates of the traits of interest, with classical pedigree breeding strategies. Thermal and infrared reflectance measures of plant canopies are examples of energy values measured by remote sensing, used to indirectly predict the selected traits.

Abstract: This invention relates to stable and genetically transformed cereal plants, other than maize or rice, that comprise an exogenous DNA expressing phosphinothricin acetyl transferase to impart resistance to phosphinothricin in said cereal plants. Cereal plants within the scope of the invention include, but are not limited to, wheat, barley and oats. The invention also pertains to transgenic cereal plants which further comprise screenable markers and transgenic seed obtained from said plants.

Abstract: This invention relates to a method for the production of stable, genetically transformed, Zea mays plants that comprise an exogenous DNA expressing phosphinothricin acetyl transferase to impart resistance to phosphinothricin in said plants. The method further comprises transforming said Zea mays plants with a second gene and further still wherein said second gene imparts insect resistance, such as the expression of a Bacillus thuringiensis toxin gene. The method for achieving stable transformation includes tissue culture methods and media, methods for the bombardment of recipient cells with said DNA's, and methods of regenerating fertile plants from transformed cells. The invention also pertains to a method of breeding said transgenic Zea mays plants with a either a non-transgenic plant or a transgenic plant, and the obtention of fertile transgenic plants and seed therefrom.

Abstract: This invention relates to a method for the production of stable, genetically transformed cereal plants, other than maize or rice, that comprise an exogenous DNA expressing phosphinothricin acetyl transferase to impart resistance to phosphinothricin in said cereal plants. Cereal plants within the scope of the method include, but are not limited to, wheat, barley and oats. The invention also pertains to a method of breeding said transgenic cereal plants with a either a non-transgenic plant or a transgenic plant, and the obtention of fertile transgenic plants and seed therefrom.

Abstract: Methods for classifying plants by remote sensing and image analysis technology are presented. These methods are useful for evaluating plants and for selecting plants for a plant breeding program which has as its goal to selectively alter phenotype. The methods combine the newer techniques of remote sensing technology to obtain indirect correlates of the traits of interest, with classical pedigree breeding strategies. Thermal and infrared reflectance measures of plant canopies are examples of energy values measured by remote sensing, used to indirectly predict the selected traits.

Abstract: This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.

Abstract: This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.