Abstract: Embodiments described herein generally relate to devices, systems and methods for measuring the dose remaining in a drug delivery device that is used for delivering a dose to a patient. In some embodiments, a dose measurement system for measuring the liquid volume in a container includes a plurality of light sources which are disposed and configured to emit electromagnetic radiation toward the container. A plurality of sensors are located in the apparatus that are optically coupleable to the plurality of light sources and are disposed and configured to detect the electromagnetic radiation emitted by at least a portion of the light sources. The apparatus also includes a processing unit configured to receive data representing the portion of the detected electromagnetic radiation from each of the plurality of sensors. The processing unit is further operable to convert the received data into a signature representative of the electromagnetic radiation detected by the plurality of sensors.

Abstract: Provided herein is a method of characterising a target polynucleotide as it moves with respect to a nanopore using a motor protein. Also provided are polynucleotide adapters and kits comprising such adapters. The methods, kits and adapters find use in characterising polynucleotides, for example in sequencing.

Abstract: Provided herein is a method of moving a double-stranded polynucleotide with respect to a nanopore using a motor protein. The method allows a portion of the polynucleotide to be interrogated by the pore multiple times. Also provided are polynucleotide adapters and kits comprising such adapters. The methods find use in characterising polynucleotides, for example in sequencing.

Abstract: The invention relates to a new method of characterising a target polynucleotide. The method uses a pore and a Dda helicase. The helicase controls the movement of the target polynucleotide through the pore. The invention also relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.

Abstract: The present disclosure describes, in part, compositions and methods for the treatment of NASH and NASH-associated diseases. Compositions comprising an agent able to increases the expression, activity, or level of one more of a protective miRNA, preferably miR-375 are provided that may be used for the treatment of liver and/or liver-associated disease is treated in the subject.

Abstract: The invention relates to new methods of moving helicases past spacers on polynucleotides and controlling the loading of helicases on polynucleotides. The invention also relates to new methods of characterising target polynucleotides using helicases.

Abstract: Embodiments described herein generally relate to devices, systems and methods for measuring a volume or number of doses remaining in a drug delivery device that is used for delivering a dose to a patient. In some embodiments, a dose measurement system for measuring the liquid volume in a container includes a light guide disposed and configured to reflect electromagnetic radiation toward the container. The dose measurement system also includes a light guide disposed and configured to emit electromagnetic radiation into the light guide. A plurality of sensors are located in the apparatus that are optically coupleable to the light guide and are disposed and configured to detect the electromagnetic radiation emitted by at least a portion of the light guide. The apparatus also includes a processing unit configured to receive data representing the portion of the detected electromagnetic radiation from each of the plurality of sensors.

Abstract: The invention relates to a new method of determining in a sample the presence or absence of one or more analyte members of a group of two or more analytes. The invention therefore relates to a multiplex assay for determining the presence or absence of each analyte in a group of multiple analytes. The assay uses aptamers and transmembrane pores.

Abstract: In a method for instantly issuing a personalized financial transaction card to a customer a bank employee receives customer information and card information from the customer at a branch location. The card information may include a card personal identification number (PIN) selected by the customer. The operator inputs the customer information and at least some the card information into a data processing terminal at the branch. The customer information and the card information are communicated from the branch across a network to a card services provider. At the card services provider, the PIN is entered into a PIN database and a reference number associated with the customer and a PIN offset is generated. The reference number and at least some of the customer data and card data may be stored in a card file associated with the customer. The reference number is used to retrieve the PIN from the PIN database. The retrieved PIN is then used to apply calculations to the card file.

Abstract: An apparatus for measuring liquid volume in a container includes a plurality of light sources for emitting electromagnetic radiation (EMR) toward the container, a plurality of sensors optically coupleable to the plurality of light sources, each sensor of the plurality of sensors for detecting the EMR emitted by at least a portion of the plurality of light sources, a temperature sensor for measuring at least one temperature associated with a liquid in the container, and at least one processor for receiving data representative of the portion of the detected EMR from each of the plurality of sensors, comparing the at least one measured temperature to a temperature guideline to identify any temperature events associated with the received data; normalizing the received data based on any temperature events associated with the received data; and converting the normalized data into a signature representative of the EMR detected by the plurality of sensors.

Abstract: Droplet interfaces are formed between droplets in an electro-wetting device comprising an array of actuation electrodes. Actuation signals are applied to selected actuation electrodes to place the droplets into an energised state in which the shape of the droplets is modified compared to a shape of the droplets in a lower energy state and to bring the two droplets into proximity. The actuation signals are then changed to lower the energy of the droplets into the lower energy state so that the droplets relax into the gap and the two droplets contact each other thereby forming a droplet interface. The use of sensing electrodes in the device permit electrical current measurements across the droplet interface. The sensing electrodes can be used for either (i) applying a reference signal during droplet actuation or (ii) recording electrical current measurements.

Abstract: A multi-stage process for reducing the environmental contaminants in an ISO8217 Table 2 compliant Feedstock Heavy Marine Fuel Oil involving a core desulfurizing process and a Detrimental Solids Removal Unit as a pre-treating step or post-treating step to the core process. The product of the process is a Product Heavy Marine Fuel Oil compliant with ISO 8217 Table 2 for residual marine fuel including a maximum sulfur content (ISO 14596 or ISO 8754) less than 0.5 wt % and a Detrimental Solids content less than 60 mg/kg. A device for conducting the process and producing the product is disclosed.

Abstract: The invention relates to a new method of determining the presence, absence or one or more characteristics of multiple analytes. The invention concerns coupling a first analyte to a membrane containing a detector and investigating the first analyte using the detector. The invention also concerns coupling a second analyte to the membrane and investigating the second analyte. The first analyte is uncoupled form the membrane prior to investigating the second analyte. The invention also relates to polynucleotide sequencing.

Abstract: In one embodiment, a method for emitting a sound from an in-ear speaker worn by a subject includes generating, by an audio source of the in-ear speaker, a source audio signal. One or more speakers of the in-ear speaker may emit a sound based on the audio signal and an audio-transport tube of the in-ear speaker may receive the sound. The one or more speakers may be comprised of a singular speaker or a speaker array coupled to a crossover network. The audio-transport tube has an input end coupled to the one or more speakers to receive the sound. An audio reflector of the in-ear speaker may reflect the sound. The audio reflector is coupled to an output end of the audio-transport tube.

Abstract: The invention relates to a method for modifying a template double stranded polynucleotide, especially for characterisation using nanopore sequencing. The method produces from the template a plurality of modified double stranded polynucleotides. These modified polynucleotides can then be characterised.

Abstract: A real time aiming assembly includes a firearm that has a barrel and a grip. A firearm motion sensor is integrated into the firearm and the firearm motion sensor is calibrated to sense a firing axis of the barrel to determine the target of the firearm. A pair of glasses is wearable on a user's face and a glasses motion sensor is integrated into the pair of glasses. The glasses motion sensor is calibrated to sense a viewing axis of the pair of glasses to determine the line of the user. A display unit is integrated into the glasses and the display unit is in communication with the glasses motion sensor. The display unit projects a dot onto a respective one of the lenses to display precisely where the bullet fired from the firearm will strike.

Abstract: A system includes a concentration sensor, a flow sensor, and a controller. The concentration sensor is configured to measure a concentration of a contaminant in a cabin of an aircraft. The flow sensor is configured to measure a flow rate of air into the cabin. The controller is configured to determine whether a concentration measurement of the contaminant in the cabin exceeds a first concentration threshold. The controller is configured to, in response to determining that the concentration measurement does not exceed the first concentration threshold, control the flow rate of air into the cabin based on a flow rate setpoint. The controller is configured to, in response to determining that the concentration measurement exceeds the first concentration threshold, control the flow rate of air into the cabin based on a flow rate setpoint and a correction factor that is based on a flow sensor tolerance.

Abstract: The invention relates to a new method of characterising a target ribonucleic acid (RNA) involving forming a complementary polynucleotide. The method uses a transmembrane pore.

Abstract: A cooking system for cooking food is provided, the system including a housing defining a hollow chamber configured to receive a food container. The housing has an upper portion defining an opening to the hollow chamber. A lid is configured to cover the upper portion of the housing and the opening to the hollow chamber in a closed position. The lid is moveable to an open position where the lid does not cover the opening to the hollow chamber. At least one heating element is associated with at least one of the housing and the lid. The cooking system is operable in a plurality of modes including a pressure cooking mode and a dry cooking mode. In the pressure cooking mode, the cooking system is operable as a pressure cooker and in the dry cooking mode, the cooking system is operable as a dry cooker.

Abstract: A multi-stage process for transforming a high sulfur ISO 8217 compliant Feedstock Heavy Marine Fuel Oil involving a core desulfurizing process that produces a Product Heavy Marine Fuel Oil that can be used as a feedstock for subsequent refinery process such as anode grade coking, needle coking and fluid catalytic cracking. The Product Heavy Marine Fuel Oil exhibits multiple properties desirable as a feedstock for those processes including a sulfur level has a maximum sulfur content (ISO 14596 or ISO 8754) between the range of 0.05 mass % to 1.0 mass. A process plant for conducting the process is also disclosed.