Abstract: An in-line sterilization system including a caddie sized and shaped to releasably secure a container and a sterilization system for sterilizing the container using pulsed ultraviolet light. The sterilization system includes a first light source and a second light source positioned within an antechamber and forming a sterilization zone between the first and second light sources, each of the first and second light sources configured to transmit pulsed ultraviolet light into the sterilization zone. The sterilization system includes a transport system having a turntable. The perimeter region of the turntable is arranged to support the caddie upon receipt of the caddie within the antechamber and to move the caddie through the sterilization zone. The caddie and at least the perimeter region of the turntable are transparent to the ultraviolet light. Related systems, devices, compositions, and methods of use are provided.