Abstract: The present disclosure provides novel nucleic acid-guided nucleases and methods of using the nucleases for genome editing. The present disclosure further provides a system for editing a target region in a genome comprising a nucleic acid-guided nuclease, a heterologous guide nucleic acid for complexing with the nucleic acid-guided nuclease, and an editing polynucleotide configured to bind to the target region.

Abstract: An optical therapeutic device comprising a front body portion, a light source provided in said front body portion, a spherical, translucent quartz lens provided in front of said light source, a bezel provided on said front body portion and configured to hold said spherical, translucent quartz lens such that a portion of the quartz lens protrudes outside the rim of the bezel. Also described is a method of using the device to apply light stimulation to acupuncture points of a human or animal body and a method for manufacturing the device.

Abstract: An optical therapeutic device comprising a front body portion, a light source provided in said front body portion, a spherical, translucent quartz lens provided in front of said light source, a bezel provided on said front body portion and configured to hold said spherical, translucent quartz lens such that a portion of the quartz lens protrudes outside the rim of the bezel. Also described is a method of using the device to apply light stimulation to acupuncture points of a human or animal body and a method for manufacturing the device.

Abstract: A bioreactor (30) for production and harvesting of microalgae is described, comprising a reactor basin (32) in the form of a tank arranged to receive CO2 and water as well as algae, and which is equipped with at least one outlet for harvesting of algal biomass, where the reactor basin (32) comprises a rotating beam (54) equipped with one or more tillable mixing grates (60) and harvesting grates (62).

Abstract: A microreactor for use in a microscope, comprising a first and second cove layer (13), which cover layers are both at least partly transparent to an electron beam (14) of an electron microscope, and extend next to each other at a mutual distance from each other and between which a chamber (15) is enclosed, wherein an inlet (4) and an outlet (5) are provided for feeding fluid through the chamber and wherein heating means (8) are provided for heating the chamber and/or elements present therein.

Abstract: A computer program product and a computer-implemented method are disclosed. The method includes providing a square design structure matrix having a number of rows and a number of columns equal to the number of rows, wherein each row represents one of a plurality of elements, wherein each entry of the design structure matrix is a numerical quantity; providing a domain mapping matrix having a number of rows equal to the number of rows of the design structure matrix, each row having an identical representation of one of the plurality of elements as a corresponding row of the design structure matrix, the domain mapping matrix having any number of columns, wherein each entry of the domain mapping matrix is a numerical quantity; and using a computer processor to group one or more elements into a cluster arrangement, using a plurality of entries from each of the matrices.

Abstract: In one embodiment of the invention a method for detecting low frequency occurrence of one or more HIV sequence variants associated with drug resistance is describe that comprises generating cDNA species from RNA molecules in an HIV sample population; amplifying first amplicons from the cDNA species, wherein each amplicon comprises amplified copies and is amplified with a pair of nucleic acid primers that define a locus; clonally amplifying the amplified copies of the first amplicons to produce second amplicons that comprise an immobilized population of substantially identical copies from one of the amplified copies of first amplicons; determining a nucleic acid sequence composition from at least 100 of the immobilized populations in parallel on a single instrument; detecting one or more sequence variants that occur at a frequency of 5% or less in the nucleic acid sequence composition of the at least 100 immobilized populations; and correlating the detected sequence variants with variation associated with HIV

Abstract: A computer program product and a computer-implemented method are disclosed. The method includes providing a square design structure matrix having a number of rows and a number of columns equal to the number of rows, wherein each row represents one of a plurality of elements, wherein each entry of the design structure matrix is a numerical quantity; providing a domain mapping matrix having a number of rows equal to the number of rows of the design structure matrix, each row having an identical representation of one of the plurality of elements as a corresponding row of the design structure matrix, the domain mapping matrix having any number of columns, wherein each entry of the domain mapping matrix is a numerical quantity; and using a computer processor to group one or more elements into a cluster arrangement, using a plurality of entries from each of the matrices.

Abstract: In one embodiment of the invention a method for detecting low frequency occurrence of one or more HIV sequence variants associated with drug resistance is describe that comprises generating cDNA species from RNA molecules in an HIV sample population; amplifying first amplicons from the cDNA species, wherein each amplicon comprises amplified copies and is amplified with a pair of nucleic acid primers that define a locus; clonally amplifying the amplified copies of the first amplicons to produce second amplicons that comprise an immobilized population of substantially identical copies from one of the amplified copies of first amplicons; determining a nucleic acid sequence composition from at least 100 of the immobilized populations in parallel on a single instrument; detecting one or more sequence variants that occur at a frequency of 5% or less in the nucleic acid sequence composition of the at least 100 immobilized populations; and correlating the detected sequence variants with variation associated with HIV

Abstract: The claimed invention provides for new sample preparation methods enabling direct sequencing of PCR products using pyrophosphate sequencing techniques. The PCR products may be specific regions of a genome. The techniques provided in this disclosure allows for SNP (single nucleotide polymorphism) detection, classification, and assessment of individual allelic polymorphisms in one individual or a population of individuals. The results may be used for diagnostic and treatment of patients as well as assessment of viral and bacterial population identification.

Abstract: An embodiment of an identifier element for identifying an origin of a template nucleic acid molecule is described that comprises a nucleic acid element comprising a sequence composition that enables detection of an introduced error in sequence data generated from the nucleic acid element and correction of the introduced error, where the nucleic acid element is constructed to couple with the end of a template nucleic acid molecule and identifies an origin of the template nucleic acid molecule.

Abstract: In one embodiment of the invention a method for detecting low frequency occurrence of one or more HIV sequence variants associated with drug resistance is describe that comprises generating cDNA species from RNA molecules in an HIV sample population; amplifying first amplicons from the cDNA species, wherein each amplicon comprises amplified copies and is amplified with a pair of nucleic acid primers that define a locus; clonally amplifying the amplified copies of the first amplicons to produce second amplicons that comprise an immobilized population of substantially identical copies from one of the amplified copies of first amplicons; determining a nucleic acid sequence composition from at least 100 of the immobilized populations in parallel on a single instrument; detecting one or more sequence variants that occur at a frequency of 5% or less in the nucleic acid sequence composition of the at least 100 immobilized populations; and correlating the detected sequence variants with variation associated with HIV

Abstract: The present invention provides for a method of preparing a target nucleic acid fragments to produce a smaller nucleic acid which comprises the two ends of the target nucleic acid. Specifically, the invention provides cloning and DNA manipulation strategies to isolate the two ends of a large target nucleic acid into a single small DNA construct for rapid cloning, sequencing, or amplification.

Abstract: An embodiment of an identifier element for identifying an origin of a template nucleic acid molecule is described that comprises a nucleic acid element comprising a sequence composition that enables detection of an introduced error in sequence data generated from the nucleic acid element and correction of the introduced error, where the nucleic acid element is constructed to couple with the end of a template nucleic acid molecule and identifies an origin of the template nucleic acid molecule.

Abstract: A microreactor for use in a microscope, comprising a first and second cove layer (13), which cover layers are both at least partly transparent to an electron beam (14) of an electron microscope, and extend next to each other at a mutual distance from each other and between which a chamber (15) is enclosed, wherein an inlet (4) and an outlet (5) are provided for feeding fluid through the chamber and wherein heating means (8) are provided for heating the chamber and/or elements present therein.

Abstract: Antisense compounds, compositions and methods are provided for modulating the expression of H-Ras, WNT-7B, acetylglucosaminyltransferase, voltage-gated K channel, IL-8, ion transport, Map3K8 and Thymidine kinase. The compositions comprise antisense compounds, particularly antisense oligonucleotides, targeted to nucleic acids encoding H-Ras, WNT-7B, acetylglucosaminyltransferase, voltage-gated K channel, IL-8, ion transport, Map3K8 and Thymidine kinase. Methods of using these compounds for modulation of H-Ras, WNT-7B, acetylglucosaminyltransferase, voltage-gated K channel, IL-8, ion transport, Map3K8 and Thymidine kinase.expression and for treatment of diseases associated with expression of H-Ras, WNT-7B, acetylglucosaminyltransferase, voltage-gated K channel, 1L-8, ion transport, Map3KS and Thymidine kinase are provided.

Abstract: Disclosed is a method in which DNA sequences derived from polysome-associated mRNA sequences in a mixed sample or in an arrayed single sequence clone can be determined and classified without sequencing. The methods make use of information on the presence of carefully chosen target subsequences, typically of length from 4 to 8 base pairs, and preferably the length between target subsequences in a sample DNA sequence together with DNA sequence databases containing lists of sequences likely to be present in the sample to determine a sample sequence. The preferred method uses restriction endonucleases to recognize target subsequences and cut the sample sequence. Then carefully chosen recognition moieties are ligated to the cut fragments, the fragments amplified, and the experimental observation made. Polymerase chain reaction (PCR) is the preferred method of amplification.

Abstract: The invention relates to a method for tracking closely adjacent and time-shifted signals incoming to a RAKE-receiver (25). According to the method, the sampling positions of the RAKE-branches (25a, 25b) are not permitted to come closer to one another in time than by a predetermined minimum value when updating the branches. The invention also relates to an arrangement (24) for putting the method into effect. The arrangement includes a search unit (24a), a plurality of tracking units (24c, 24d) and a processor unit (24b). The invention also relates to a RAKE-receiver utilizing said apparatus.

Abstract: The present invention comprises an assembly of a substantially block-shaped object and four packaging elements for placing over one or more edge parts formed by a short edge of the object, wherein the packaging elements are each manufactured from one piece of at least slightly flexible foamed plastic and each comprise:a first engaging surface for engaging on a surface of the object which extends on one end of the edge substantially transversely thereof, anda second engaging surface for engaging on a second surface of the object which extends substantially parallel to the first surface on the other end of the edge.