Patents by Inventor Jan Grawe
Jan Grawe has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11860350Abstract: A method for performing microscopy-based imaging of samples includes: loading a sample holder onto a support configured to receive the sample holder; moving the sample holder in a first direction, from a starting position on a first strip of the sample holder, to move the sample holder relative to an imaging line of a line camera, to capture an image of the first strip of the sample holder; monitoring a focal plane using an autofocus system as the sample holder is moved in the first direction; in response to a determination by the autofocus system, moving an objective lens along an optical axis to adjust the focal plane; and moving the sample holder in a second direction, to align the imaging line of the line camera with a position on a second strip of the sample holder.Type: GrantFiled: July 20, 2022Date of Patent: January 2, 2024Assignee: Q-LINEA ABInventors: Jonas Jarvius, Jan Grawé
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Patent number: 11845978Abstract: A method for detecting and characterising a microorganism in a clinical sample includes introducing the clinical sample to a first culture vessel and simultaneously transporting and incubating the clinical sample in a thermally insulated compartment of a sealable container. A test aliquot is removed from the sample and the remainder of the sample is further cultured. DNA is removed from the test aliquot and nucleic acid tests are performed on it to identify the microorganism and to detect the presence or absence of one or more genetic antimicrobial resistance markers in the microorganism. One or more nucleic acid probes or primers are used to perform the nucleic acid tests. It is detected whether or not the probes or primers have hybridised to DNA or the primers have been extended. If a microorganism is identified, then an antimicrobial susceptibility test is performed on the cultured clinical sample.Type: GrantFiled: April 21, 2017Date of Patent: December 19, 2023Assignee: Q-LINEA ABInventors: Jonas Jarvius, Anders Lind, Henrik Soderstrom, Jan Grawe
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Publication number: 20220382030Abstract: A method for performing microscopy-based imaging of samples comprises: loading a sample holder (100) onto a support (50) configured to receive the sample holder (100); moving the sample holder (100) in a first direction, from a starting position on a first strip of the sample holder (100), to move the sample holder (100) relative to an imaging line of a line camera (10), to capture an image of the first strip of the sample holder (100); monitoring a focal plane using an autofocus system (15) as the sample holder (100) is moved in the first direction; in response to a signal from the autofocus system (15), moving an objective lens (25) along the optical axis to adjust the focal plane; and moving the sample holder (100) in a second direction, to align the imaging line of the line camera (10) with a position on a second strip of the sample holder (100).Type: ApplicationFiled: July 20, 2022Publication date: December 1, 2022Applicant: Q-LINEA ABInventors: Jonas JARVIUS, Jan GRAWÉ
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Patent number: 11505835Abstract: A method for detecting and characterizing a microorganism in a clinical sample includes introducing a clinical sample to a first culture vessel containing the culture medium; removing a test aliquot; separating DNA from the test aliquot; and performing nucleic acid tests on the DNA to identify the microorganism and to detect the presence or absence of one or more genetic antimicrobial resistance markers in the microorganism. If a microorganism is identified, an antimicrobial susceptibility test is performed wherein microbial growth in the antimicrobial susceptibility test is monitored by accessing growth or markers for growth and wherein the type and concentration of antimicrobial agents used in the antimicrobial susceptibility test is determined by the identity of the microorganism and the antimicrobial resistance markers detected. A device for performing the method is also provided.Type: GrantFiled: April 9, 2020Date of Patent: November 22, 2022Assignee: Q-LINEA ABInventors: Jonas Jarvius, Jan Grawe
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Patent number: 11455719Abstract: A method of image-based analysis of multiple samples includes using a sample holder having multiple locations of interest and multiple focal structures that are each associated, one or more, with the multiple locations of interest, wherein the multiple samples are dispersed across the multiple locations of interest and obtaining image areas of the multiple locations of interest. Multiple digital image areas are thus obtained for use in an analysis of the multiple samples with each of the image areas including at least one of the locations of interest and at least one of the focal structures. An image processing algorithm is used to analyse each of the digital image areas and check if the focal structure indicates that the image area is in clear focus. An indication is provided and/or remedial action is taken if the image processing algorithm indicates that any digital image areas are out of focus.Type: GrantFiled: June 15, 2017Date of Patent: September 27, 2022Assignee: Q-LINEA ABInventors: Jonas Jarvius, Jan Grawe
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Patent number: 11428917Abstract: A method for performing microscopy-based imaging of samples comprises: loading a sample holder (100) onto a support (50) configured to receive the sample holder (100); moving the sample holder (100) in a first direction, from a starting position on a first strip of the sample holder (100), to move the sample holder (100) relative to an imaging line of a line camera (10), to capture an image of the first strip of the sample holder (100); monitoring a focal plane using an autofocus system (15) as the sample holder (100) is moved in the first direction; in response to a signal from the autofocus system (15), moving an objective lens (25) along the optical axis to adjust the focal plane; and moving the sample holder (100) in a second direction, to align the imaging line of the line camera (10) with a position on a second strip of the sample holder (100).Type: GrantFiled: December 18, 2018Date of Patent: August 30, 2022Assignee: Q-LINEA ABInventors: Jonas Jarvius, Jan Grawé
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Patent number: 11338286Abstract: A substrate for use in manufacture of a production master plate for production of a detection disc for carrying samples in an apparatus for detection of microscopic objects in a fluid, the substrate having a channel and separate focus structure, wherein the focus structure is a groove.Type: GrantFiled: September 25, 2018Date of Patent: May 24, 2022Assignee: Q-LINEA ABInventors: Jonas Sven Peter Jarvius, Jan Grawe
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Publication number: 20210246410Abstract: A portable apparatus and method for transport and incubation of a medical sample in a blood culture flask includes a sealable container having a thermally insulated compartment for receiving the blood culture flask and a heater for heating the medical sample to a temperature suitable for pre-culturing of the sample. An agitator is provided for agitating the sample in the blood culture flask.Type: ApplicationFiled: March 26, 2021Publication date: August 12, 2021Applicant: Q-linea ABInventors: Jonas Jarvius, Anders Lind, Henrik Soderstrom, Jan Grawe, Ian Fitzpatrick, Jurg Bartholdi
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Publication number: 20210224978Abstract: A method of image-based analysis of multiple samples includes using a sample holder having multiple locations of interest and multiple focal structures that are each associated, one or more, with the multiple locations of interest, wherein the multiple samples are dispersed across the multiple locations of interest and obtaining image areas of the multiple locations of interest. Multiple digital image areas are thus obtained for use in an analysis of the multiple samples with each of the image areas including at least one of the locations of interest and at least one of the focal structures. An image processing algorithm is used to analyse each of the digital image areas and check if the focal structure indicates that the image area is in clear focus. An indication is provided and/or remedial action is taken if the image processing algorithm indicates that any digital image areas are out of focus.Type: ApplicationFiled: June 15, 2017Publication date: July 22, 2021Applicant: Q-linea ABInventors: Jonas Jarvius, Jan Grawe
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Publication number: 20210154663Abstract: An apparatus for moving a sample holder 110 on a platform 652 from a loading position, where the sample holder 110 can be removed from the platform 652, to a locked position, where the sample holder 110 is securely held, wherein the sample holder 110 rests on wheels 690a within a recessed portion 651 on the platform 652, wherein the apparatus is configured such that movement from the loading position to the locked position causes a vertical clamping means 653 to lower down on top of the sample holder 110, and a horizontal clamping 657 means to be pressed to the outer periphery of the sample holder 110, and such that that movement from the locked position to the loading position causes the vertical clamping means 653 to raise above the sample holder 110, and the horizontal clamping means 657 to be moved away from the outer periphery of the sample holder 110.Type: ApplicationFiled: April 23, 2019Publication date: May 27, 2021Applicant: Q-LINEA ABInventors: Anders LIND, Henrik SÖDERSTRÖM, Henrik SVANBERG, Jan GRAWÉ, Johan SKABORN
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Patent number: 10995311Abstract: A portable apparatus for transport and incubation of a medical sample in a blood culture flask includes a sealable container having a thermally insulated compartment for receiving the blood culture flask and a heater for heating the medical sample to a temperature suitable for pre-culturing of the sample. An agitator is provided for agitating the sample in the blood culture flask.Type: GrantFiled: April 21, 2016Date of Patent: May 4, 2021Assignee: Q-linea ABInventors: Jonas Jarvius, Anders Lind, Henrik Soderstrom, Jan Grawe, Ian Fitzpatrick, Jurg Bartholdi
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Publication number: 20210032674Abstract: The present invention provides a method of preparing a suspension of intact microorganisms from a sample containing microorganisms and mammalian cells, comprising contacting the sample with a buffer solution with a pH of at least pH 6 and less than pH 9, a detergent and one or more proteases to allow lysis of the mammalian cells; filtering the mixture through a filter suitable for retaining microorganisms to remove the lysed mammalian cells; resuspending the microorganisms retained by the filter in a liquid to provide a suspension comprising the recovered intact microorganisms; and determining the concentration of microorganisms in the suspension by: i. heating and/or contacting an aliquot of the suspension with an alcohol; ii. optionally diluting one or more aliquots of the suspension to provide one or more diluted aliquots before, during and/or after step (i); iii. contacting at least a portion of an aliquot of step (i) or (ii) with a single fluorescent stain capable of binding to DNA; iv.Type: ApplicationFiled: January 22, 2019Publication date: February 4, 2021Applicant: Q-LINEA ABInventors: Jan GRAWÉ, Markus KLINTSTEDT
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Publication number: 20210017568Abstract: The present invention provides methods for determining the antimicrobial susceptibility of a microorganism in a clinical sample said method comprising removing a test aliquot from a clinical sample culture before the culture reaches 0.5 McFarland units, isolating the microbial cells and transferring the cells into a suitable culture medium for microbial growth, and performing an AST assay using the isolated microbes, wherein the concentration of microbial cells in the microbial cells used to set up the AST assay is measured before the degree of microbial growth in the different growth conditions of the AST assay is measured. Devices for determining the antimicrobial susceptibility of a microorganism in a clinical sample are also provided.Type: ApplicationFiled: October 1, 2020Publication date: January 21, 2021Applicant: Q-linea ABInventors: Jonas Jarvius, Jan Grawe, Ylva Molin, Markus Klintstedt, Mats Gullberg
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Publication number: 20200363616Abstract: A method for performing microscopy-based imaging of samples comprises: loading a sample holder (100) onto a support (50) configured to receive the sample holder (100); moving the sample holder (100) in a first direction, from a starting position on a first strip of the sample holder (100), to move the sample holder (100) relative to an imaging line of a line camera (10), to capture an image of the first strip of the sample holder (100); monitoring a focal plane using an autofocus system (15) as the sample holder (100) is moved in the first direction; in response to a signal from the autofocus system (15), moving an objective lens (25) along the optical axis to adjust the focal plane; and moving the sample holder (100) in a second direction, to align the imaging line of the line camera (10) with a position on a second strip of the sample holder (100).Type: ApplicationFiled: December 18, 2018Publication date: November 19, 2020Applicant: Q-LINEA ABInventors: Jonas JARVIUS, Jan GRAWÉ
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Patent number: 10829797Abstract: A method for determining the antimicrobial susceptibility of a microorganism in a clinical sample includes removing a test aliquot from a clinical sample culture before the culture reaches 0.5 McFarland units and isolating the microbial cells. The cells are transferred into a suitable culture medium for microbial growth and an AST assay is performed using the isolated microbes. The concentration of microbial cells in the microbial cells used to set up the AST assay is measured before measuring the degree of microbial growth in different conditions of the AST assay. Devices for determining the anti-microbial susceptibility of a microorganism in a clinical sample are also disclosed.Type: GrantFiled: June 16, 2016Date of Patent: November 10, 2020Assignee: Q-linea ABInventors: Jonas Jarvius, Jan Grawe, Ylva Molin, Markus Klintstedt, Mats Gullberg
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Patent number: 10831011Abstract: A sample holder 100 for use in a device for image-based analysis of multiple samples, the sample holder comprising: an optically flat surface 10; multiple locations of interest 16, 18 dispersed across the optically flat surface 10 that each, in use, correspond to locations of a sample of the multiple samples; and multiple focal structures 12 associated with the multiple locations of interest 16, 18; wherein the focal structures 12 each comprise at least one pyramid shaped indentation 30 in the optically flat surface 10.Type: GrantFiled: June 15, 2017Date of Patent: November 10, 2020Assignee: Q-Linea ABInventors: Jonas Jarvius, Jan Grawe
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Publication number: 20200318164Abstract: A method for detecting and characterizing a microorganism in a clinical sample includes introducing a clinical sample to a first culture vessel containing the culture medium; removing a test aliquot; separating DNA from the test aliquot; and performing nucleic acid tests on the DNA to identify the microorganism and to detect the presence or absence of one or more genetic antimicrobial resistance markers in the microorganism. If a microorganism is identified, an antimicrobial susceptibility test is performed wherein microbial growth in the antimicrobial susceptibility test is monitored by accessing growth or markers for growth and wherein the type and concentration of antimicrobial agents used in the antimicrobial susceptibility test is determined by the identity of the microorganism and the antimicrobial resistance markers detected. A device for performing the method is also provided.Type: ApplicationFiled: April 9, 2020Publication date: October 8, 2020Applicant: Q-linea ABInventors: Jonas Jarvius, Jan Grawe
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Publication number: 20200299748Abstract: The present invention relates to a method of determining the concentration of intact microorganisms in a sample comprising optionally diluting an aliquot of the sample to provide a diluted aliquot at a dilution value; contacting at least a portion of an aliquot or of a diluted aliquot of the sample with first and second stains capable of binding to DNA, wherein the first stain is a fluorescent stain, is cell-permeable, and has a first emission wavelength, and the second stain is cell-impermeable, and is capable of acting as an acceptor molecule in a FRET pair with the first stain acting as a donor molecule; imaging the aliquot-stain mixture at the first emission wavelength and determining an image analysis value for the number of objects corresponding to intact microorganisms in the imaged mixture; and comparing the image analysis value for said aliquot to a pre-determined calibration curve, as well as an apparatus, a consumable and a kit therefor.Type: ApplicationFiled: October 12, 2018Publication date: September 24, 2020Applicant: Q-LINEA ABInventors: Jonas JARVIUS, Jan GRAWE, Markus KLINTSTEDT
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Patent number: D1016317Type: GrantFiled: November 19, 2020Date of Patent: February 27, 2024Assignee: Q-LINEA ABInventors: Jonas Jarvius, Jan Grawé, Mats Gullberg, Jerker Persson, Karin Granström
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Patent number: D1019981Type: GrantFiled: April 27, 2022Date of Patent: March 26, 2024Assignee: Q-LINEA ABInventors: Anders Lind, Caroline Johansson, Daniel Dolk Djurberg, Henrik Soderstrom, Jan Grawe, Jonas Jarvius, Jonas Melin, Karl Skold, Magnus Sandow, Markus Lindblom, Richard Kroon, Sara Frolander, Sebastian Carlsson, Simon Uhrberg, Ake Strese, Magnus Forslund