Abstract: A special-purpose cuvette assembly with features that create a small, restricted volume to minimize bulk movements of liquid and minimize backscattering-induced broadening of light. The special-purpose cuvette assembly enables recording of Brownian movements of nanoparticles in a liquid when it is placed in a suitable optical device comprising a light sheet and an optical microscope attached to a video camera that is oriented in a direction perpendicular to the light-sheet plane.

Abstract: The disclosure provides for a novel system for generating a high dynamic range video of a colloid under investigation. This helps record images of various sizes of nanoparticles that scatter light with very different efficiencies (effective cross-sections), typically orders of magnitude different.

Abstract: The system includes an adjustable light source constructed to direct a beam of electromagnetic radiation at a specimen chamber that allows a portion of the beam to scatter when illuminating particles within the chamber. The scattered portion of the beam is directed to a sensor, the sensor having a frame rate and a time period between frames. The system may have a processor connected to the sensor and light source, the processor may perform the following steps: activate the light source and obtain images from sensor; if the images from the sensor show that particles are blinking then reduce the frame rate, set the exposure time to at least 60% of the time between frames and reduce the illumination. Then the processor obtains additional images and processes those images to mitigate blurring. The processor determines the Brownian motion of the particles from the processed images and determines the sizes of the particles based on the motion.

Abstract: The disclosure provides for a novel optical chopper that can rapidly change the intensity of light incident on a colloid under investigation. This helps record images of various sizes of nanoparticles that scatter light with very different efficiencies (effective cross-sections), typically orders of magnitude different.

Abstract: The disclosure provides for a novel optical chopper that can rapidly change the intensity of light incident on a colloid under investigation. This helps recording various sizes of nanoparticles that scatter light with very different efficiencies (effective cross-sections), typically orders of magnitude different.

Abstract: A particle image analyzer is disclosed that includes a transparent moving structure with a load surface and an opposite surface, where a portion of the load surface is constructed to adhere particles. A particle discharge nozzle deposits particles on the load surface and an image sensor positioned adjacent to the load surface takes images of the particles as they move past the image sensor. A light source positioned adjacent to the opposite surface illuminates the particles imaged by the image sensor. The light from the light source defines an illumination path that travels from the light source, through the opposite surface, through the load surface and to the image sensor.

Abstract: The system includes an adjustable light source constructed to direct a beam of electromagnetic radiation at a specimen chamber that allows a portion of the beam to scatter when illuminating particles within the chamber. The scattered portion of the beam is directed to a sensor, the sensor having a frame rate and a time period between frames. The system may have a processor connected to the sensor and light source, the processor may perform the following steps: activate the light source and obtain images from sensor; if the images from the sensor show that particles are blinking then reduce the frame rate, set the exposure time to at least 60% of the time between frames and reduce the illumination. Then, the processor obtains additional images and processes those images to mitigate blurring. The processor determines the Brownian motion of the particles from the processed images and determines the sizes of the particles based on the motion.

Abstract: Disclosed herein is a true random number generator (TRNG). The TRNG includes a cavity filled with tritium and an electronic sensor constructed to detect energy from the decay of the tritium. The sensor produces a signal for the detected energy, and an amplifier amplifies the signal while a filter filters the signal. A processor (a) determines whether the signal represents decay events for tritium; (b) sets a timer to determine the time period between decay events; (c) based on the time period in step (b), assigns a value of a 0 or a 1; (d) stores the value in a memory; (e) repeats steps (b)-(d), resulting in a string of values; and (f) generates a true random number based on the string of values. This TRNG may be formed on an integrated circuit.

Abstract: Disclosed herein is a true random number generator (TRNG). The TRNG includes a cavity filled with tritium and an electronic sensor constructed to detect energy from the decay of the tritium. The sensor produces a signal for the detected energy, and an amplifier amplifies the signal while a filter filters the signal. A processor (a) determines whether the signal represents decay events for tritium; (b) sets a timer to determine the time period between decay events; (c) based on the time period in step (b), assigns a value of a 0 or a 1; (d) stores the value in a memory; (e) repeats steps (b)-(d), resulting in a string of values; and (f) generates a true random number based on the string of values. This TRNG may be formed on an integrated circuit.

Abstract: A system for calculating the volume of a plurality of particles is disclosed. The system includes three optical assemblies, each with a light source directing a beam of light of a wavelength along an optical axis at a specimen chamber, a filter positioned along the optical path after the chamber, and a sensor positioned along the optical path after the filter. The wavelength of each beam of light is different from the other wavelengths, and each optical axis is orthogonal to the other optical axes. A processor is connected to the sensors in each of the optical assemblies, and the processors perform the following steps: (a) capture an image from each of the three sensors; (b) based on the images, calibrate each sensor; (c) after calibration, capture an image from each of the three sensors; and (d) determine a volume of the plurality of particles, based on the images captured in step (c).

Abstract: A system for emitting and detecting electromagnetic radiation of multiple wavelengths to observe the motion of particles in a polydisperse solution in order to size the particles is provided. The system includes a first and second light sources constructed to emit a first and second beams of electromagnetic radiation at substantially a first and second wavelength, respectively. The beams are directed to a specimen chamber such that a portion of the beams scatter when illuminating the particles, and wherein the scattered portion of the beams are directed to a sensor. The first and second wavelengths are different from each other and a recorder is connected to the sensor. At processor controls the light sources in a time-division fashion, and from the resulting images the size of particles can be determined by tracking the motion of the particles.

Abstract: A system for determining the growth/dissolution rate of colloidal particles is disclosed and includes multiple light sources and multiple sensors. A light source is constructed to emit a beam of electromagnetic radiation at a specimen chamber that holds the colloidal particles. The chamber allows a portion of the combined beam to scatter perpendicularly or at some other angle to the combined beam. The scattered portion of the beam is directed to a sensor that detects electromagnetic radiation. The sensor is connected to processor that activates the light source and obtains an image from the sensor. Multiple images are taken at a time interval and for each image taken, and a total image intensity level is calculated and normalized. A formula is then calculated that fits the normalized values over time and a slope is determined from the formula.

Abstract: A system and method are provided to observe and count particles in polydisperse solutions with dark field microscopy while distinguishing among particles of different sizes and accurately counting particles. A calibration mask, calibration light source, and multiple wavelengths of light are used. Opaque calibration marks on the transparent calibration mask define a region of interest. Multiple beams of various wavelengths are combined into a beam or a light sheet and the perpendicular component of scattered light from the specimen particles is then split into separate wavelengths and detected by separate sensors attuned to each wavelength.

Abstract: A method for calibrating a dark field microcopy setup is disclosed. The method includes preparing a plurality of particle samples, each with a known concentration and particle size, the plurality having more than one particle size and, optionally, more than one refractive index and more than one diluent. For each sample in the plurality, the sample is measured in the setup and the scattered light intensity and number of particles is measured. From this data, a relationship between the scattered light intensity, particle size and calibrated investigated volume can be determined. The calibrated investigated volume is used to obtain the proper particle size distribution in a given diluent.

Abstract: A method for calibrating a dark field microcopy setup is disclosed. The method includes preparing a plurality of particle samples, each with a known concentration and particle size, the plurality having more than one particle size and, optionally, more than one refractive index and more than one diluent. For each sample in the plurality, the sample is measured in the setup and the scattered light intensity and number of particles is measured. From this data, a relationship between the scattered light intensity, particle size and calibrated investigated volume can be determined. The calibrated investigated volume is used to obtain the proper particle size distribution in a given diluent.

Abstract: A special purpose cuvette assembly with features that create a small, restricted volume to minimize bulk movements of liquid and that minimize backscattering of light. The special-purpose cuvette assembly enables recording of Brownian movements of nanoparticles in a liquid when it is placed in a suitable optical device comprising a light sheet and an optical microscope attached to a video camera that is oriented perpendicular to the light-sheet plane.

Abstract: The system and cuvette insert is disclosed where the insert has a top surface that includes a first and second vertical channel opening. A first vertical channel extends downwardly from the first vertical channel opening and a second vertical channel extending from the second vertical channel opening. The insert also has a side wall into which the viewing chamber is formed. The viewing chamber has an upper viewing chamber wall and a lower viewing chamber wall. These walls define the viewing chamber and may be substantially parallel to the floor of the cuvette into which the insert is inserted. At the end of the viewing chamber is a reflecting wall. The viewing chamber has two ends, with one end in fluid connection with the first vertical channel and the other end in fluid connection with the second vertical channel. The fluid connection between the viewing chamber and the first vertical channel may also include a first lateral channel.

Abstract: A system and method are provided to observe and count particles in polydisperse solutions with dark field microscopy while distinguishing among particles of different sizes and accurately counting particles. A calibration mask, calibration light source, and multiple wavelengths of light are used. Opaque calibration marks on the transparent calibration mask define a region of interest. Multiple beams of various wavelengths are combined into a beam or a light sheet and the perpendicular component of scattered light from the specimen particles is then split into separate wavelengths and detected by separate sensors attuned to each wavelength.

Abstract: Methods for detecting and analyzing individual nanoparticles of the same, similar, or different sizes co-existing in a fluid sample using multi-spectral analysis are disclosed. A plurality of light sources may be configured to produce a plurality of light beams at different spectral wavebands. An optical assembly may be configured to combine the plurality of light beams into one or more incident light sheets. Each incident light sheet may illuminate one or more nanoparticles in a liquid sample. One or more image detectors may be configured to detect, using a plurality of wavelengths, light scattered or emitted by one or more nanoparticles. The plurality of wavelengths may correspond to the different spectral wavebands of the plurality of light beams. Related apparatus, systems, techniques, and articles are also described.

Abstract: A system for determining the growth/dissolution rate of colloidal particles is disclosed and includes multiple light sources and multiple sensors. A light source is constructed to emit a beam of electromagnetic radiation at a specimen chamber that holds the colloidal particles. The chamber allows a portion of the combined beam to scatter perpendicularly or at some other angle to the combined beam. The scattered portion of the beam is directed to a sensor that detects electromagnetic radiation. The sensor is connected to processor that activates the light source and obtains an image from the sensor. Multiple images are taken at a time interval and for each image taken, and a total image intensity level is calculated and normalized. A formula is then calculated that fits the normalized values over time and a slope is determined from the formula.